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Analysis of EST sequences suggests recent origin of allotetraploid colonial and creeping bentgrasses

Molecular Genetics and Genomics volume 278, pages 197–209 (2007)Cite this article

Abstract

Advances in plant genomics have permitted the analysis of several members of the grass family, including the major domesticated species, and provided new insights into the evolution of the major crops on earth. Two members, colonial bentgrass (Agrostis capillaris L.) and creeping bentgrass (A. stolonifera L.) have only recently been domesticated and provide an interesting case of polyploidy and comparison to crops that have undergone human selection for thousands of years. As an initial step of characterizing these genomes, we have sampled roughly 10% of their gene content, thereby also serving as a starting point for the construction of their physical and genetic maps. Sampling mRNA from plants subjected to environmental stress showed a remarkable increase in transcription of transposable elements. Both colonial and creeping bentgrass are allotetraploids and are considered to have one genome in common, designated the A2 genome. Analysis of conserved genes present among the ESTs suggests the colonial and creeping bentgrass A2 genomes diverged from a common ancestor approximately 2.2 million years ago (MYA), thereby providing an enhanced evolutionary zoom in respect to the origin of maize, which formed 4.8 MYA, and tetraploid wheat, which formed only 0.5 MYA and is the progenitor of domesticated hexaploid wheat.

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Acknowledgments

This work was supported with funds provided by The Rutgers Center for Turfgrass Science, The United States Department of Agriculture, Michigan State University, and the United States Golf Association. We thank M. Murillo and G. Zylstra for assistance with the colony picking. We thank G. Fuks, G. Keizer, A.B. Nelson, S. Young, and V. Zohovetz at PGIR for technical assistance.

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Affiliations

  1. Department of Plant Biology and Pathology and The Biotechnology Center for Agriculture & the Environment, School of Environmental and Biological Sciences, Rutgers, The State University of New Jersey, New Brunswick, NJ, 08903, USA

    David Rotter, Huaijun Michael Li, Chongyuan Luo, Stacy A. Bonos, William A. Meyer & Faith C. Belanger

  2. The Plant Genome Initiative at Rutgers, Waksman Institute, Rutgers, The State University of New Jersey, Piscataway, NJ, 08854, USA

    Arvind K. Bharti & Joachim Messing

  3. Department of Crop and Soil Science, Michigan State University, East Lansing, MI, 48824, USA

    Suleiman Bughrara

  4. Department of Plant, Soil, and Insect Sciences, University of Massachusetts-Amherst, Amherst, MA, 01003, USA

    Geunhwa Jung

  5. Turku Center for Biotechnology, Tykistoekatu 6, Turku, Finland

    Stephen Rudd

  6. USDA-ARS, Beltsville, MD, 20705, USA

    Scott E. Warnke

Authors
  1. David Rotter
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  2. Arvind K. Bharti
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  3. Huaijun Michael Li
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  4. Chongyuan Luo
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  5. Stacy A. Bonos
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  6. Suleiman Bughrara
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  7. Geunhwa Jung
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  8. Joachim Messing
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  9. William A. Meyer
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  10. Stephen Rudd
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  11. Scott E. Warnke
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  12. Faith C. Belanger
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Corresponding author

Correspondence to Faith C. Belanger.

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Communicated by R. Hagemann.

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Rotter, D., Bharti, A.K., Li, H.M. et al. Analysis of EST sequences suggests recent origin of allotetraploid colonial and creeping bentgrasses. Mol Genet Genomics 278, 197–209 (2007). https://doi.org/10.1007/s00438-007-0240-2

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  • DOI: https://doi.org/10.1007/s00438-007-0240-2

Keywords

  • Agrostis
  • Evolution
  • Grass genomics
  • Polyploidy
  • Environmental stress