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An Arabidopsis mutant that is resistant to the protoporphyrinogen oxidase inhibitor acifluorfen shows regulatory changes in tetrapyrrole biosynthesis

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Abstract

Several Arabidopsis mutants of the ecotype Dijon were isolated that show resistance to the herbicide acifluorfen, which inactivates protoporphyrinogen oxidase (PPOX), an enzyme of tetrapyrrole biosynthesis. This enzyme provides protoporphyrin for both Mg chelatase and ferrochelatase at the branchpoint, which leads to chlorophyll and heme, respectively. One of the mutations, aci5-3, displays semidominant inheritance. Heterozygous progeny showed yellow-green leaves, while the homozygous seedlings were white and inviable, but could be rescued by supplementing the medium with sugar. Interestingly, the expression of neither of the two forms of PPOX was altered in the mutant, but the rate of synthesis of 5-aminolevulinate, the precursor of all tetrapyrroles, was drastically reduced. Genetic mapping revealed the mutant locus is closely linked to the ch42 marker, which is itself located in the CHLI-1 gene which codes for one of the three subunits of Mg chelatase. The cs mutant also shows a defect in this gene, and test for allelism with aci5-3 confirmed that the two mutations are allelic. Sequencing of the wild type and aci5-3 alleles of CHLI-1 revealed a single base change (G718A), which results in a D240N substitution in the CHLI-1 protein. In the homozygous aci5-3 mutant no CHLI-1 RNA or protein could be detected. Strikingly, CHLH and CHLI-2 transcripts were also absent. This indicates the existence of a feedback-regulatory mechanism that inactivates the genes encoding certain Mg chelatase subunits. The basis for the semidominant inheritance pattern and the relationship between herbicide resistance and modified gene expression is discussed.

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Authors and Affiliations

  1. Department of Genetics, Faculty of Biology, M.V. Lomonosov State University, Leninskie Gori 1, Building 12, 119899, Moscow, Russia

    Olga Soldatova, Alexey Apchelimov, Natalia Radukina, Tatiana Ezhova & Sergey Shestakov

  2. Department of Plant Physiology, Institute of Biology, Humboldt University, Philippstreet 13, Building 12, 10115, Berlin, Germany

    Valeria Ziemann, Boris Hedtke & Bernhard Grimm

Authors
  1. Olga Soldatova
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  2. Alexey Apchelimov
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  3. Natalia Radukina
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  4. Tatiana Ezhova
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  5. Sergey Shestakov
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  6. Valeria Ziemann
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  7. Boris Hedtke
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  8. Bernhard Grimm
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Correspondence to Bernhard Grimm.

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R. Herrmann

O. Soldatova and V. Ziemann contributed equally to this work.

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Soldatova, O., Apchelimov, A., Radukina, N. et al. An Arabidopsis mutant that is resistant to the protoporphyrinogen oxidase inhibitor acifluorfen shows regulatory changes in tetrapyrrole biosynthesis. Mol Genet Genomics 273, 311–318 (2005). https://doi.org/10.1007/s00438-005-1129-6

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  • DOI: https://doi.org/10.1007/s00438-005-1129-6

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