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Functional analysis of the BIN2 genes of cotton

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Abstract

Brassinosteroids (BR) promote the elongation of cotton fibers and may be a factor in determining their final length. To begin to understand the role of BR-mediated responses in the development of cotton fibers we have characterized the BIN2 genes of cotton. BIN2 is a member of the shaggy-like protein kinase family that has been identified as a negative regulator of BR signaling in Arabidopsis. Sequence analyses indicate that the tetraploid cotton genome includes four genes with strong sequence similarity to BIN2. These genes fall into two distinct subclasses based on sequence and expression patterns. Sequence comparisons with corresponding genes from cotton species that have the diploid A and D genomes, respectively, shows that each pair of genes comprises homeologs derived from the A and D sub-genomes. Transgenic Arabidopsis plants that express these cotton BIN2 cDNAs show reduced growth and similar phenotypes to the semi-dominant bin2 mutant plants. These results indicate that the cotton BIN2 genes encode functional BIN2 isoforms that can inhibit BR signaling. Further analyses of the function of BIN2 genes and their possible roles in determining fiber yield and quality are underway.

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Acknowledgements

We wish to thank Ms. Jing Wang for development of the cotton boll cDNA library. We also thank Dr. Jianming Li for providing us with the BIN2 cDNA sequence prior to publication. We also thank Dr. Robert Bradley for assistance with sequence alignments. This research was supported by USDA-NRI Grant No. 2003-35304-13384

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Authors and Affiliations

  1. Department of Biological Sciences, Texas Tech University, Lubbock, TX, 79409-3131, USA

    Yan Sun

  2. Department of Plant and Soil Sciences, Texas Tech University, Lubbock, TX, 79409, USA

    Randy D. Allen

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  1. Yan Sun
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  2. Randy D. Allen
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Correspondence to Randy D. Allen.

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Sun, Y., Allen, R.D. Functional analysis of the BIN2 genes of cotton. Mol Genet Genomics 274, 51–59 (2005). https://doi.org/10.1007/s00438-005-1122-0

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  • DOI: https://doi.org/10.1007/s00438-005-1122-0

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