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Wide-cross whole-genome radiation hybrid mapping of the cotton (Gossypium barbadense L.) genome

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Abstract

Whole-genome radiation hybrid mapping has been applied extensively to human and certain animal species, but little to plants. We recently demonstrated an alternative mapping approach in cotton (Gossypium hirsutum L.), based on segmentation by 5-krad γ-irradiation and derivation of wide-cross whole-genome radiation hybrids (WWRHs). However, limitations observed at the 5-krad level suggested that higher doses might be advantageous. Here, we describe the development of an improved second-generation WWRH panel after higher dose irradiation and compare the resulting map to the 5-krad map. The genome of G. hirsutum (n=26) was used to rescue the radiation-segmented genome of G. barbadense (n=26) introduced via 8- and 12-krad γ-irradiated pollen. Viable seedlings were not recovered after 12-krad irradiation, but 8-krad irradiation permitted plant recovery and construction of a 92-member WWRH mapping panel. Assessment of 31 SSR marker loci from four chromosomes revealed that the 8-krad panel has a marker retention frequency of ca. 76%, which is approximately equivalent to the rate of loss in a low-dose animal radiation hybrid panel. Retention frequencies of loci did not depart significantly from independence when compared between the A and D subgenomes, or according to positions along individual chromosomes. WWRH maps of chromosomes 10 and 17 were generated by the maximum likelihood RHMAP program and the general retention model. The resulting maps bolster evidence that WWRH mapping complements traditional linkage mapping and works in cotton, and that the 8-krad panel complements the 5-krad panel by offering higher rates of chromosome breakages, lower marker retention frequency, and more retention patterns.

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Acknowledgements

We thank D. Raska for greenhouse culture of WWRHs; M. Boehnke’s group at University of Michigan for offering the RHMAP program; Plant Research International for offering MapChart 2.1; and the Texas A&M University Nuclear Science Center for helping the irradiation treatment. This work was supported in part by grants from the Texas Agricultural Experimental Station, Texas Biotechnology Initiative, Texas Higher Education Coordinating Board Advanced Technology Program and Cotton Incorporated.

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Author notes

  1. Wenxiang Gao

    Present address: Department of Genetics, University of Georgia, Athens, GA, 30602, USA

Authors and Affiliations

  1. Department of Soil and Crop Sciences, Texas A & M University, College Station, TX, 77843-2474, USA

    Wenxiang Gao, Z. Jeffrey Chen & David M. Stelly

  2. USDA-ARS, Germplasm Research Unit, 2765 F&B Road, College Station, TX, 77845, USA

    John Z. Yu & Russell J. Kohel

  3. Department of Veterinary Pathobiology, Texas A&M University, College Station, TX, 77843, USA

    James E. Womack

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  1. Wenxiang Gao
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  2. Z. Jeffrey Chen
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  3. John Z. Yu
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  4. Russell J. Kohel
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  5. James E. Womack
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  6. David M. Stelly
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Correspondence to David M. Stelly.

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Communicated by W.R. McCombie

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Gao, W., Chen, Z.J., Yu, J.Z. et al. Wide-cross whole-genome radiation hybrid mapping of the cotton (Gossypium barbadense L.) genome. Mol Genet Genomics 275, 105–113 (2006). https://doi.org/10.1007/s00438-005-0069-5

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  • DOI: https://doi.org/10.1007/s00438-005-0069-5

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