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Gold color in onions ( Allium cepa): a natural mutation of the chalcone isomerase gene resulting in a premature stop codon

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Abstract

Unusual gold-colored onions were selected from a F3 family originating from a cross between US-type yellow and Brazilian yellow onions. HPLC analysis showed that the gold onions contained a significantly reduced amount of quercetin, the most abundant flavonoid in onions. This result indicated that an early step in the flavonoid biosynthesis pathway might be abnormal in these onions. The expression of flavonoid synthesis genes isolated from onions was examined in gold onions and compared to that in onions of other colors by RT-PCR. The results showed that all genes were transcribed in gold onions as in red onions. In order to identify any critical mutations in flavonoid synthesis genes encoding enzymes involved in early steps of the pathway, the genomic sequence of chalcone isomerase (CHI) was obtained. A premature stop codon and a subsequent single base-pair addition causing a frameshift were identified in the coding region of the CHI gene in the gold onions. Co-segregation of the mutant allele of the CHI gene and the gold phenotype was investigated in the original F2 segregating population. Genotyping of three color groups (red, yellow and gold) of F2 onions revealed perfect co-segregation of the mutant CHI allele with the gold phenotype. All tested gold F2 onions were homozygous for the mutant CHI allele. This perfect co-segregation implies that the presence of a premature stop codon in the gold CHI gene results in an inactive CHI. Inactivation of CHI results in a block in the flavonoid biosynthesis pathway and the accumulation of chalcone derivatives, including a yellow pigment which might be responsible for the gold color in onions.

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References

  • Bastianetto S, Quirion R (2002) Natural extracts as possible protective agents of brain aging. Neurobiol Aging 5687:1–7

    Google Scholar 

  • Bharti A, Khurana J (2003) Molecular characterization of transparent testa ( tt) mutants of Arabidopsis thaliana (ecotype Estland) impaired in flavonoid biosynthesis pathway. Plant Sci 165:1321–1332

    Article  CAS  Google Scholar 

  • Braca A, Sortino C, Politi M, Morelli I, Mendez J (2002) Antioxidant activity of flavonoids from Licania licaniaeflora. J Ethnopharmacol 79:379–381

    Article  CAS  PubMed  Google Scholar 

  • Clarke AE, Jones HA, Little TM (1944) Inheritance of bulb color in the onion. Genetics 29:569–575

    Google Scholar 

  • Cook NC, Samman S (1996) Flavonoids: chemistry, metabolism, cardioprotective effects, and dietary sources. Nutr Biochem 7:66–76

    CAS  Google Scholar 

  • Davis GN, El-Shafie MW (1967) Inheritance of bulb color in the onion ( Allium cepa L.). Hilgardia 38:607–622

    Google Scholar 

  • Forkmann G, Dangelmayr B (1980) Genetic control of chalcone isomerase activity in flowers of Dianthus caryophyllus. Biochem Genet 18:519–527

    CAS  PubMed  Google Scholar 

  • Fossen T, Andersen OM, Ovstedal DO, Pedersen AT, Raknes A (1996) Characteristic anthocyanin pattern from onions and other Allium spp. J Food Sci 61:703–706

    CAS  Google Scholar 

  • Hertog MGL, Hollman PCH, Katan MB (1992) Content of potentially anticarcinogenic flavonoids of 28 vegetables and 9 fruits commonly consumed in the Netherlands. J Agr Food Chem 40:2379–2383

    CAS  Google Scholar 

  • Hollman P, Katan M (1997) Absorption, metabolism and health effects of dietary flavonoids in man. Biomed Pharmacother 51:305–310

    Article  CAS  PubMed  Google Scholar 

  • Holton TA, Cornish EC (1995) Genetics and biochemistry of anthocyanin biosynthesis. Plant Cell 7:1070–1083

    Article  Google Scholar 

  • Itoh Y, Higeta D, Suzuki A, Yoshida H, Ozeki Y (2002) Excision of transposable elements from the chalcone isomerase and dihydroflavonol 4-reductase genes may contribute to the variegation of the yellow-flowered carnation ( Dianthus caryophyllus). Plant Cell Physiol 43:578–585

    Article  CAS  PubMed  Google Scholar 

  • Jez JM, Noel JP (2002) Reaction mechanism of chalcone isomerase. J Biol Chem 277:1361–1369

    Article  CAS  PubMed  Google Scholar 

  • Jez JM, Bowman ME, Dixon RA, Noel JP (2000) Structure and mechanism of the evolutionarily unique plant enzyme chalcone isomerase. Nat Struct Biol 7:786–791

    Article  CAS  PubMed  Google Scholar 

  • Keli S, Hertog M, Feskens E, Kromhout D (1996) Flavonoids, antioxidant vitamins and risk of stroke. The Zutphen study. Arch Internal Medicine 156:637–642

    Article  CAS  Google Scholar 

  • Kim S, Binzel M, Yoo K, Park S, Pike L (2004a) Inactivation of DFR (dihydroflavonol 4-reductase) gene transcription results in blockage of anthocyanin production in yellow onions ( Allium cepa). Mol Breed, in press

  • Kim S, Binzel M, Yoo K, Park S, Pike L (2004b) Pink ( P), a new locus responsible for pink trait in onions ( Allium cepa) resulting from natural mutations of anthocyanidin synthase. Mol Genet Genomics, in press

  • Knekt P, Jarvinen R, Reunanen A, Maatela J (1996) Flavonoid intake and coronary mortality in Finland: a cohort study. Brit Medical J 312:478–481

    CAS  Google Scholar 

  • Kobayashi T, Nakata T, Kuzumaki T (2002) Effect of flavonoids on cell cycle progression in prostate cancer cells. Cancer Lett 176:17–23

    Article  CAS  PubMed  Google Scholar 

  • Kuhn B, Forkmann G, Seyffert W (1978) Genetic control of chalcone-flavanone isomerase activity in Callistephus chinensis. Planta 138:199–203

    CAS  Google Scholar 

  • Kumar S, Tamura K, Jakobsen IB, Nei M (2001) MEGA2: molecular evolutionary genetics analysis software. Bioinformatics 17:1244–1255

    Article  CAS  PubMed  Google Scholar 

  • Li J, Ou-Lee T, Raba R, Amundson R, Last R (1993) Arabidopsis flavonoid mutants are hypersensitive to UV-B irradiation. Plant Cell 5:171–179

    Article  CAS  PubMed  Google Scholar 

  • Muir SR, Collins GJ, Robinson S, Hughes S, Bovy A, Vos CHR, van Tunen A, Verhoeyen ME (2001) Overexpression of petunia chalcone isomerase in tomato results in fruit containing increased levels of flavonols. Nat Biotechnol 19:470–474

    Article  CAS  PubMed  Google Scholar 

  • Patil B, Pike L, Yoo K (1995) Variation in the quercetin content in different colored onions ( Allium cepa L.). J Amer Soc Hort Sci 120:909–913

    CAS  Google Scholar 

  • Reiman GH (1931) Genetic factors for pigmentation in the onion and their relation to disease resistance. J Agr Res 42:251–278

    Google Scholar 

  • Rhodes MJC, Price KR (1996) Analytical problems in the study of flavonoid compounds in onions. Food Chem. 57:113–117

    Google Scholar 

  • Shirley BW (1996) Flavonoid biosynthesis: ‘new’ functions for an ‘old’ pathway. Trends Plant Sci 1:377–382

    Article  Google Scholar 

  • Van Tunen AJ, Mur LA, Recourt K, Gerats AGM, Mol JNM (1991) Regulation and manipulation of flavonoid gene expression in anthers of petunia: the molecular basis of the Po mutation. Plant Cell 3:39–48

    Article  PubMed  Google Scholar 

  • Yamazaki M, Makita Y, Springob K, Saito K (2003) Regulatory mechanisms for anthocyanin biosynthesis in chemotypes of Perilla frutescens var. crispa. Biochem Eng J 14:191–197

    Article  CAS  Google Scholar 

  • Zeback R, Dressler K, Hess D (1989) Flavonoid compounds from pollen and stigma of Petunia hybrida: inducers of the vir region of the Agrobacterium tumefaciens Ti plasmid. Plant Sci 62:83–91

    Article  Google Scholar 

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Acknowledgements

The authors thank members of the Vegetable & Fruit Improvement Center for their dedicated support of this research. This work was supported by produce industry member contributions to the Vegetable & Fruit Improvement Center and by a U.S. Department of Agriculture grant (CSREES 2001-34402-10543, “Designing Foods for Health”)

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Authors and Affiliations

  1. Vegetable & Fruit Improvement Center, Department of Horticultural Sciences, Texas A&M University, College Station, TX 77845, USA

    S. Kim, R. Jones, K. S. Yoo & L. M. Pike

  2. Seminis Vegetable Seeds, Inc., 1500 Research Parkway, College Station, TX 77845, USA

    R. Jones

Authors
  1. S. Kim
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  2. R. Jones
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  3. K. S. Yoo
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  4. L. M. Pike
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Corresponding author

Correspondence to L. M. Pike.

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Communicated by R. Hagemann

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Kim, S., Jones, R., Yoo, K.S. et al. Gold color in onions ( Allium cepa): a natural mutation of the chalcone isomerase gene resulting in a premature stop codon. Mol Genet Genomics 272, 411–419 (2004). https://doi.org/10.1007/s00438-004-1076-7

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  • DOI: https://doi.org/10.1007/s00438-004-1076-7

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