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Comparative mapping between Medicago sativa and Pisum sativum

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Abstract

Comparative genome analysis has been performed between alfalfa ( Medicago sativa) and pea ( Pisum sativum), species which represent two closely related tribes of the subfamily Papilionoideae with different basic chromosome numbers. The positions of genes on the most recent linkage map of diploid alfalfa were compared to those of homologous loci on the combined genetic map of pea to analyze the degree of co-linearity between their linkage groups. In addition to using unique genes, analysis of the map positions of multicopy (homologous) genes identified syntenic homologs (characterized by similar positions on the maps) and pinpointed the positions of non-syntenic homologs. The comparison revealed extensive conservation of gene order between alfalfa and pea. However, genetic rearrangements (due to breakage and reunion) were localized which can account for the difference in chromosome number (8 for alfalfa and 7 for pea). Based on these genetic events and our increasing knowledge of the genomic structure of pea, it was concluded that the difference in genome size between the two species (the pea genome is 5- to 10-fold larger than that of alfalfa) is not a consequence of genome duplication in pea. The high degree of synteny observed between pea and Medicago loci makes further map-based cloning of pea genes based on the genome resources now available for M. truncatula a promising strategy.

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Acknowledgements

We would like to thank K. Karchesz, C. Ernhoffer, P. Somkúti, S. Jenei, Z. Liptay for skillful technical assistance; P. Kiss for help in mapping EuDicot gene-specific loci ( GAPDH, HSP70 , RPL17 , RPL32 , RPS9 , RPSA40 and UCE), Á. Dalmadi and S. Stranczinger for their help during the mapping of Uni, and PscDNA clones in alfalfa. The authors thank Michel Delseny for useful discussions. This work was supported by the ABC Gödöllő, the BRC Szeged, and the Bástyai-Holczer Foundation, and by grants from the EU EuDicotMap (Grant No. BIO 4CT97-2170; EU), and MEDICAGO (Grant No. QLG2-CT-2000-30676; EU), and by the Hungarian National Grants OMFB EU-97-D8-063 (National Committee for Technical Development), NKFP Medicago Genomics (Grant No. 4/023/2001-Ministry of Education) and Biotechnology 2001 (Grant No. OMFD-00229/2002), OTKA (Grant No.T038211). P. Kaló, A. Kereszt and G. Endre were supported by János Bolyai postdoctoral fellowships

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Authors and Affiliations

  1. Institute of Genetics, Agricultural Biotechnology Center, Szent-Györgyi A. u. 4, 2100, Gödöllő, Hungary

    P. Kaló, J. Jakab & G. B. Kiss

  2. Institute of Genetics, Biological Research Center of the Hungarian Academy of Sciences, P.O. Box 521, 6701, Szeged, Hungary

    P. Kaló, A. Seres, Z. Kevei, A. Kereszt, G. Endre & G. B. Kiss

  3. Department of Crop Genetics, John Innes Centre, Colney Lane, Norwich, NR4 7UH, UK

    S. A. Taylor & T. H. N. Ellis

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  1. P. Kaló
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  2. A. Seres
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  9. G. B. Kiss
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Correspondence to G. B. Kiss.

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Communicated by W. R. McCombie

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Kaló, P., Seres, A., Taylor, S.A. et al. Comparative mapping between Medicago sativa and Pisum sativum. Mol Genet Genomics 272, 235–246 (2004). https://doi.org/10.1007/s00438-004-1055-z

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