Abstract
In this study, we show that the Saccharomyces cerevisiae ORF YBR142w, which encodes a putative DEAD-box RNA helicase, corresponds to MAK5. The mak5-1 allele is deficient in the maintenance of the M1 dsRNA virus, resulting in a killer minus phenotype. This allele carries two mutations, G218D in the conserved ATPase A-motif and P618S in a non-conserved region. We have separated these mutations and shown that it is the G218D mutation that is responsible for the killer minus phenotype. Mak5p is an essential nucleolar protein; depletion of the protein leads to a reduction in the level of 60S ribosomal subunits, the appearance of half-mer polysomes, and a delay in production of the mature 25S and 5.8S rRNAs. Thus, Mak5p is involved in the biogenesis of 60S ribosomal subunits.
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Acknowledgements
We would like to thank Prof. F. Lacroute for many helpful discussions, Prof. R. B. Wickner for providing strains and Dr. O. Gadal for the Nop1dsRED construction. This work was supported by grants from the Polish-French Center for Plant Biotechnology, the CNRS and Grant No. 6PO4A00919 from the State Committee for Scientific Research, Poland. D.K. was supported by a grant from the Swiss National Science Foundation to P. Linder
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Zagulski, M., Kressler, D., Bécam, AM. et al. Mak5p, which is required for the maintenance of the M1 dsRNA virus, is encoded by the yeast ORF YBR142w and is involved in the biogenesis of the 60S subunit of the ribosome. Mol Genet Genomics 270, 216–224 (2003). https://doi.org/10.1007/s00438-003-0913-4
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DOI: https://doi.org/10.1007/s00438-003-0913-4