Molecular Genetics and Genomics

, Volume 269, Issue 5, pp 583–591 | Cite as

Virus-induced silencing of WIPK and SIPK genes reduces resistance to a bacterial pathogen, but has no effect on the INF1-induced hypersensitive response (HR) in Nicotiana benthamiana

  • P. C. Sharma
  • A. Ito
  • T. Shimizu
  • R. Terauchi
  • S. Kamoun
  • H. SaitohEmail author
Original Paper


Activation of two mitogen-activated protein kinases (MAPKs), wound-induced protein kinase (WIPK) and salicylic acid-induced protein kinase (SIPK), is one of the earliest responses that occur in tobacco plants that have been wounded, treated with pathogen-derived elicitors or challenged with avirulent pathogens. We isolated cDNAs for these MAPKs ( NbWIPK and NbSIPK) from Nicotiana benthamiana. The function of NbWIPK and NbSIPK in mediating the hypersensitive response (HR) triggered by infiltration with INF1 protein (the major elicitin secreted by Phytophthora infestans), and the defense response to an incompatible bacterial pathogen ( Pseudomonas cichorii), was investigated by employing virus-induced gene silencing (VIGS) to inhibit expression of the WIPK and SIPK genes in N. benthamiana. Silencing of WIPK or SIPK, or both genes simultaneously, resulted in reduced resistance to P. cichorii, but no change was observed in the timing or extent of HR development after treatment with INF1.


Nicotiana benthamiana WIPK/SIPK INF1  Pseudomonas cichorii Virus-induced gene silencing 



We thank Dr. D. C. Baulcombe (Sainsbury Laboratory, John Innes Centre) for the gift of plasmids pPC2S and pTXS.GFP. We are grateful to Dr. S. Seo, Dr. Y. Ohashi (National Institute of Agrobiological Resources) for the provision of anti-WIPK antibody and for invaluable suggestions. We thank Dr. T. Romeis (Max-Planck-Institute for Plant Breeding Research), Dr. H. Yoshioka (Graduate School of Bioagricultural Sciences, Nagoya University) and Dr. Y. Hikichi (Faculty of Agriculture, Kochi University) for invaluable suggestions. PSC is thanks the JSPS for financial help in the form of an Invitation Research Fellowship. This work was carried out in a containment facility of Iwate Biotechnology Research Center under License No. 13-YokoShoku-965 from the Ministry of Agriculture, Forestry and Fisheries, Japan, and License No. 12-Ken-Kyoku-52 from the Ministry of Education, Culture and Science, Japan. This study was in part supported by the Research for the Future program of the Japan Society for the Promotion of Science


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Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  • P. C. Sharma
    • 1
    • 5
  • A. Ito
    • 2
  • T. Shimizu
    • 2
  • R. Terauchi
    • 2
  • S. Kamoun
    • 3
  • H. Saitoh
    • 2
    • 4
    • 6
    Email author
  1. 1.Department of Agricultural BotanyCh. Charan Singh UniversityMeerutIndia
  2. 2.Iwate Biotechnology Research CenterKitakamiJapan
  3. 3.Department of Plant Pathology, Ohio Agricultural Research and Development CenterOhio State UniversityWoosterUSA
  4. 4.Japan Society for the Promotion of ScienceTokyo 102-8471Japan
  5. 5.School of BiotechnologyGuru Gobind Singh Indraprastha UniversityDelhiIndia
  6. 6.Department of Plant Microbe InteractionsMax-Planck-Institute for Plant Breeding ResearchCologneGermany

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