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A bacterial artificial chromosome (BAC) library of sugar beet and a physical map of the region encompassing the bolting gene B

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Abstract

In sugar beet ( Beta vulgaris L.), early bolting is caused by a single dominant gene, designated B. Twenty AFLP markers selected from a 7.8-cM segment of the B region on chromosome 2 were used to screen a YAC library, and a first-generation physical map including the B gene, made up of 11 YACs, was established. Because the genome coverage of the YAC library was low, a BAC library was constructed in the vector pBeloBAC11. This library consists of 57,600 clones with an average insert size of 116 kb, corresponding to 8.8 genome equivalents. Screening of the BAC library with chloroplast and mitochondrial DNA probes indicated that less than 0.1% of the clones contained organelle-derived DNA. To fill the gaps in the physical map around the B gene, the BAC library was screened with four AFLP markers and 10 YAC-derived probes. In total, 54 different BACs were identified. Overlaps between BACs were detected by using BAC termini amplified by PCR as probes, and by RFLP fingerprinting. In this way, a minimal tiling path of the central 4.6-cM region was constructed, which consists of 14 BACs. The B locus was localized to a 360-kb contig, a size which makes positional cloning of the gene feasible.

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Acknowledgements

We would like to thank Claudia Radüge and Monika Bruisch for their excellent technical assistance. The ndhA, coxI, and 23S rRNA probes were kindly provided by K. Krupinska. The clones for the Arabidopsis genes FRI and FCA were made available by C. Dean. The FLC clone was a generous gift from C. Sheldon. The collaboration with M. Ganal and D. Schmidt in picking and gridding of the BAC library at the IPK Gatersleben is gratefully acknowledged. We thank M. Ganal and F.P. Wolter for critical reading of the manuscript. This project is funded by the seed companies Strube-Dieckmann (Nienstädt, Germany) and KWS SAAT AG (Einbeck, Germany) and the German Federal Ministry for Education, Research and Technology BMBF (GABI Grant Nos. 0312283A and 0312284). S. S. Alam is grateful for support by the Alexander von Humboldt Foundation

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  1. S. Alam

    Present address: Department of Botany, University of Dhaka, 1000, Dhaka, Bangladesh

Authors and Affiliations

  1. Institute for Crop Science and Plant Breeding, Christian-Albrechts-University of Kiel, Olshausenstr. 40, 24098 , Kiel, Germany

    U. Hohmann, G. Jacobs, A. Telgmann, R. M. Gaafar, S. Alam & C. Jung

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  1. U. Hohmann
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  2. G. Jacobs
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Correspondence to C. Jung.

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Communicated by R. Hagemann

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Hohmann, U., Jacobs, G., Telgmann, A. et al. A bacterial artificial chromosome (BAC) library of sugar beet and a physical map of the region encompassing the bolting gene B . Mol Gen Genomics 269, 126–136 (2003). https://doi.org/10.1007/s00438-003-0821-7

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