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Comparative analysis of superfamilies of NBS-encoding disease resistance gene analogs in cultivated and wild apple species

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Abstract

Eleven distinct families of resistance gene analogs (RGAs) with the characteristic nucleotide-binding sequence (NBS) were identified in two wild apple species, Malus prunifolia and M. baccata, and two cultivated apple cultivars, M. domestica cv. Fuji and M. domestica cv. Hong-ok, using PCR approaches with degenerate primers based on two conserved motifs of known NBS-LRR resistance genes. These RGA families were found to be represented in all the apple species tested, including wild and cultivated species. However, their sequences are very divergent from each other. Furthermore, the low level of recombination detected within their RGA families supports the idea that the evolution of NBS-encoding sequences in apple species involves the gradual accumulation of mutations. Despite the high diversity of the RGA families found in all apple species, the apparent lack of differentiation between wild and cultivated forms suggests that other factors, such as the capacity to tolerate pathogens, might play an important role in the survival of wild-type species.

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Acknowledgements

We are grateful to Dr. Y. E. Shin (National Horticultural Research Institute, Korea) for leaves collected from apple trees. This work was supported in part by a special grant research program of the Ministry of Agriculture and Forestry of Korea (1997), by a grant from the Brain Korea 21 project (2001), and by a grant from the 21st frontier R&D program (CG124).

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Authors and Affiliations

  1. Department of Life Sciences, Ewha Womans University, 120-750, Seoul, Korea

    S.-Y. Lee, J.-S. Seo, M. Rodriguez-Lanetty & D.-H. Lee

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  1. S.-Y. Lee
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  2. J.-S. Seo
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  3. M. Rodriguez-Lanetty
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  4. D.-H. Lee
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Correspondence to D.-H. Lee.

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Communicated by R. Hagemann

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Lee, SY., Seo, JS., Rodriguez-Lanetty, M. et al. Comparative analysis of superfamilies of NBS-encoding disease resistance gene analogs in cultivated and wild apple species. Mol Gen Genomics 269, 101–108 (2003). https://doi.org/10.1007/s00438-003-0816-4

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  • DOI: https://doi.org/10.1007/s00438-003-0816-4

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