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The ATPase ClpX is conditionally involved in the morphological differentiation of Streptomyces lividans

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Abstract

ATP-dependent proteases of the ClpP type are widespread in eubacteria. These proteolytic complexes are composed of a proteolytic subunit and an ATPase subunit. They are involved in the degradation of denatured proteins, but also play a role in specific regulatory pathways. In Streptomyces lividans strains which lack the proteolytic subunit ClpP1, cell cycle progression has been shown to be blocked at early stages of growth. In this study, we examined the role of the ATPase subunit ClpX, a possible partner of the products of the clpP1 operon. A clpX mutant was obtained and it was shown that its growth was impaired only on acidic medium. Thus, the clpX phenotype differs from the clpP1 phenotype, indicating that these two components have only partially overlapping roles. We also analyzed the expression of clpX. Although clpX expression is increased under heat-shock conditions in many bacteria, we found that this is not the case in S. lividans.

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Acknowledgements

We are grateful to T. Msadek and T. Mignot for fruitful discussions. We thank G. Rapoport for his comments on the manuscript. We are grateful to Edith Gouin and Roger Zenon for their help in obtaining polyclonal anti-ClpX antibodies in rabbit. We thank A. Edelman and associates for correcting this manuscript. This work was supported by research funds from the Institut Pasteur, Centre National de Recherche Scientifique and the Université Paris 7. J.V. was the recipient of a fellowship from the Ministère de l'Education Nationale, de la Recherche et de la Technologie

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  1. Unité de Biochimie Microbienne, Institut Pasteur, 25 rue du Docteur Roux, 75724 Cedex 15, Paris , France

    J. Viala & P. Mazodier

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  1. J. Viala
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  2. P. Mazodier
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Correspondence to P. Mazodier.

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Communicated by A. Kondorosi

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Viala, J., Mazodier, P. The ATPase ClpX is conditionally involved in the morphological differentiation of Streptomyces lividans . Mol Gen Genomics 268, 563–569 (2003). https://doi.org/10.1007/s00438-002-0783-1

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