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Attempt to differentiate Leishmania (Leishmania) amazonensis, L. (L.) chagasi, Leishmania (Viannia) braziliensis and L. (V.) guyanensis using the SSR-PCR technique

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Abstract.

The ability to differentiate reference strains of Leishmania. (Leishmania) amazonensis, L. (L.) chagasi, Leishmania (Viannia) braziliensis and L. (V.) guyanensis was evaluated using the simple sequence repeat polymerase chain reaction (SSR-PCR) technique. This technique differentiates the Leishmania species, generating distinct DNA amplicon profiles. The SSR-PCR profiles were similar to but more reproducible than those produced by RAPD. SSR-PCR is presented as an alternative to other molecular methods for the differentiation of Leishmania species or strains.

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Authors and Affiliations

  1. Laboratório de Parasitologia Celular Molecular do Centro de Pesquisas René Rachou/Fundacao Oswaldo Cruz, Ave Augusto de Lima 1715, Barro Preto, Belo Horizonte-MG, Brazil, , , , ,

    Ângela Volpini & Alvaro Romanha

  2. Departmento de Clínica Médica da Faculdade de Medicina-UFMG and Laboratório de Epidemiologia e Antropologia Médica, CPqRR/FIOCRUZ, Belo Horizonte-MG, Brazil, , , , ,

    Valéria Azeredo Passos

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  1. Ângela Volpini
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  2. Valéria Azeredo Passos
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  3. Alvaro Romanha
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Volpini, Â., Azeredo Passos, V. & Romanha, A. Attempt to differentiate Leishmania (Leishmania) amazonensis, L. (L.) chagasi, Leishmania (Viannia) braziliensis and L. (V.) guyanensis using the SSR-PCR technique. Parasitol Res 87, 1056–1059 (2001). https://doi.org/10.1007/s004360100469

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  • DOI: https://doi.org/10.1007/s004360100469

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