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Unusual organization of the 5S ribosomal spacer in Dirofilaria repens: absence of a canonical spliced leader 1 sequence

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Abstract

Comparing the 5S ribosomal RNA spacer of Dirofilaria repens and D. immitis, we found that in D. repens PCR generates two amplification products. In both products, the putative spliced leader 1 sequences (SL1) show some nucleotide substitutions compared to the canonical SL1 sequence of nematodes. SL1 is a short sequence transferred to the 5′ end of most messenger RNAs through a trans-splicing reaction. All the other filariae studied thus far amplify a single fragment showing a canonical SL1 sequence. D. repens appears thus peculiar.

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Authors and Affiliations

  1. Istituto di Patologia Generale Veterinaria dell'Università degli Studi di Milano, via Celoria 10, 20133 Milan, Italy e-mail: favia@axrma.uniroma1.it Fax: +39-2-2364470, , , , , , IT

    Guido Favia, Chiara Bazzocchi, Claudio Genchi & Claudio Bandi

  2. Istituto di Parassitologia, Fondazione Cenci-Bolognetti, Universita' “La Sapienza”, Piazzale Aldo Moro 5, 00185 Rome, Italy, , , , , , IT

    Gabriella Cancrini

Authors
  1. Guido Favia
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  2. Chiara Bazzocchi
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  3. Gabriella Cancrini
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  4. Claudio Genchi
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  5. Claudio Bandi
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Received: 5 September 1999 / Accepted: 1 December 1999

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Favia, G., Bazzocchi, C., Cancrini, G. et al. Unusual organization of the 5S ribosomal spacer in Dirofilaria repens: absence of a canonical spliced leader 1 sequence. Parasitol Res 86, 497–499 (2000). https://doi.org/10.1007/s004360050700

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  • DOI: https://doi.org/10.1007/s004360050700

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