Abstract
Experiments were undertaken to develop a serum-free medium for the in vitro cultivation of Babesia caballi, a tick-borne hemoprotozoan parasite, one of the causative agents of equine piroplasmosis. A modified HL-1 medium supplemented with horse serum, L-glutamine, antibiotics, and hypoxanthine was used. B. caballi organisms were continuously cultivated at 37 °C in microaerophilous stationary-phase culture in a humidified atmosphere containing 5% CO2 in air before exposure to serum-free culture conditions. For serum-free propagation, lipid-rich bovine serum albumin (LR-BSA), alone or with chemically defined lipids (CDL), were added instead of serum. Media containing LR-BSA alone or LR-BSA and CDL in various amounts supported the in vitro propagation of B. caballi. Growth was maintained for more than 6 months. The growth rates obtained in serum-free media were similar to those previously obtained in traditional serum-containing medium.
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Received: 30 June 1998 / Accepted: 29 September 1998
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Zweygarth, E., van Niekerk, C. & de Waal, D. Continuous in vitro cultivation of Babesia caballi in serum-free medium. Parasitol Res 85, 413–416 (1999). https://doi.org/10.1007/s004360050568
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DOI: https://doi.org/10.1007/s004360050568