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Development of Blastocystis hominis cysts into vacuolar forms in vitro

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Abstract

The development of cysts of Blastocystis hominis isolated from human feces by the Ficoll-Paque concentration method and cultured in Jones' medium containing 10% horse serum is described. The morphological changes were studied by light and transmission electron microscopy at different intervals for up to 48 h. The cysts developed into a large number of vacuolar forms within 24 h, and binary fission was the only mode of reproduction observed.

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Authors and Affiliations

  1. Department of Microbiology, Faculty of Medicine, National University of Singapore, 10 Kent Ridge Crescent, Singapore 119260 E-mail: micmulki@nus.edu.sg, Fax: +65-77-66 872, , , , , , SG

    K. T. Moe, M. Singh, J. Howe, L. C. Ho, S. W. Tan, X. Q. Chen & E. H. Yap

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  1. K. T. Moe
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  2. M. Singh
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  3. J. Howe
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  4. L. C. Ho
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  5. S. W. Tan
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  6. X. Q. Chen
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  7. E. H. Yap
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Received: 29 May 1998 / Accepted: 3 July 1998

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Moe, K., Singh, M., Howe, J. et al. Development of Blastocystis hominis cysts into vacuolar forms in vitro. Parasitol Res 85, 103–108 (1999). https://doi.org/10.1007/s004360050517

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  • DOI: https://doi.org/10.1007/s004360050517

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