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Amplification of genomic DNA fragments of Sarcocystis muris (Apicomplexa) cyst merozoites encoding a thiol (cysteine) proteinase

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Abstract

Parasites of the phylum Apicomplexa (Sporozoa) cause diseases such as malaria, toxoplasmosis, or intestinal coccidiosis. Invasive stages possess typical apical organelles such as dense granules that harbor a broad range of polypeptides that are believed to take part in the parasite-host cell interaction. In previous studies a 26-kDa polypeptide of dense granules from Sarcocystis muris cyst merozoites (bradyzoites) was characterized as a thiol (cysteine) proteinase. In this paper a method is demonstrated to amplify DNA fragments from genomic DNA of S. muris cyst merozoites by polymerase chain reaction, which probably code for the 26-kDa antigen.

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Authors and Affiliations

  1. Ruhr Universität Bochum, Arbeitsgruppe Molekulare Genetik, D-44780 Bochum, Germany Tel.: +49-234 700-3102; Fax: +49-234 7094-195; e-mail: wolfgang.rueger@ruhr-uni-bochum.de, , , , , , DE

    Thomas Hansner, Bernd Freyer & Wolfgang Rüger

  2. Heinrich Heine Universität, Institut für Zoomorphologie, Zellbiologie und Parasitologie, D-40225 Düsseldorf, Germany, , , , , , DE

    Heinz Mehlhorn

Authors
  1. Thomas Hansner
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  2. Bernd Freyer
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  3. Heinz Mehlhorn
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  4. Wolfgang Rüger
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Received: 12 December 1997 / Accepted: 17 January 1998

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Hansner, T., Freyer, B., Mehlhorn, H. et al. Amplification of genomic DNA fragments of Sarcocystis muris (Apicomplexa) cyst merozoites encoding a thiol (cysteine) proteinase. Parasitol Res 84, 578–582 (1998). https://doi.org/10.1007/s004360050452

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  • DOI: https://doi.org/10.1007/s004360050452

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