Skip to main content
SpringerLink
Log in

A new method for isolation and differentiation of native Entamoeba histolytica and E. dispar cysts from fecal samples

  • ORIGINAL PAPER
  • Published:
Parasitology Research Aims and scope Submit manuscript

Abstract

A new method for the purification of protozoan cysts from feces was established, allowing the isolation of native cysts. The procedure consists of two sucrose-density gradients and enzymatic digestion of cellulose particles by cellulase and can be accomplished in a few hours. The cyst fractions were differentiated into Entamoeba histolytica and E. dispar using the DNA probes P145 and B133 in a dot-blot test.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Author information

Authors and Affiliations

  1. Institut für Tropenmedizin, Forschungslaboratorien, Wilhelmstrasse 27, D-72074 Tübingen, Germany Tel.: +49/7071/2982929; Fax: +49/7071/293017; E-mail: brigitte.walderich@uni-tuebingen.de, , , , , , DE

    B. Walderich, L. Müller & J. Knobloch

  2. Weizmann Institute of Science, Rehovot 76100, Israel, , , , , , IL

    R. Bracha

  3. Bernhard Nocht Institut, Bernhard-Nocht-Straßsse 74, D-20359 Hamburg, Germany, , , , , , DE

    G. D. Burchard

Authors
  1. B. Walderich
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. L. Müller
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. R. Bracha
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. J. Knobloch
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. G. D. Burchard
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Received: 7 March 1997 / Accepted: 24 March 1997

Rights and permissions

Reprints and permissions

About this article

Cite this article

Walderich, B., Müller, L., Bracha, R. et al. A new method for isolation and differentiation of native Entamoeba histolytica and E. dispar cysts from fecal samples. Parasitol Res 83, 719–721 (1997). https://doi.org/10.1007/s004360050326

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/s004360050326

Keywords

Search

Navigation