Abstract
The malic dehydrogenase (MDH) isoenzymatic pattern of Dictyocaulus viviparus, Protostrongylus rufescens, and Slovakian and Spanish isolates of D. filaria was studied. The MDH isoenzymatic pattern in both isolates of D. filaria was characterized by the presence of three phenotypes: (1) a single, slow anodic band; (2) a single, fast anodic band; and (3) a large spot matching its migration with bands 1 and 2. These three phenotypes may be explained as the existence of only one gene locus for the MDH in D. filaria. Allelic frequencies and the Hardy-Weinberg test were determined for Slovakian and Spanish isolates of D. filaria. This test indicated that the population was not in Hardy-Weinberg equilibrium. The MDH isoenzymatic pattern of D. viviparus displayed the same phenotypes 1 and 2 observed in D. filaria. Furthermore, the MDH isoenzymatic pattern of P. rufescens was characterized by the presence of two bands with anodic and cathodic migration. The isoenzyme with anodic migration appeared more intensively stained than did that with cathodic migration. This last isoenzyme was not observed when the samples had been stored for 1 month.
This is a preview of subscription content, log in via an institution to check access.
References
Agatsuma T, Suzuki N (1980a) Electrophoretic studies on enzymes in the Japanese common liver fluke, Fasciola spp. I. Enzyme variations in the natural population. Jpn J Med Sci Biol 33:249–254
Agatsuma T, Suzuki N (1980b) Electrophoretic studies on enzymes in Paragonimus spp. I. Comparison of isozyme patterns between P. ohirai and P. miyazakii. Jpn J Parasitol 30:7–13
Coles GC (1970) A comparison of some isoenzymes of Schistosoma mansoni and S. haematobium. Comp Biochem Physiol 33:549–558
Cutillas C, Espina C, Spakulová M, Arias P (1995) Differential diagnosis of lung nematode parasites from livestock by electrophoretic techniques. Int J Parasitol 25:215–220
Flockhart HA (1982) The identification of some Onchocerca spp. of cattle by isoenzyme analysis. Trop Med Parasitol 33:51–56
Le Riche PD, Sewell MMH (1978) Differentiation of Taeniid cestodes by enzyme electrophoresis. Int J Parasitol 8:479–83
McManus DP, Smyth JD (1979) Isoelectric focusing of some enzymes from Echinococcus granulosus (horse and sheep strains) and E. multilocularis. Trans R Soc Trop Med Hyg 73:259–265
Pasteur N, Pasteur G, Bonhomme F, Catalán J, Britton-Davidian J (1988) Practical isozyme genetics. Ellis Horwood, London
Price PW (1977) General concepts on the evolutionary biology of parasites. Evolution 31:405–420
Price PW (1980) Evolutionary biology of parasites. Princeton University Press, Princeton
Richardson BJ, Baverstock PR, Adams M (1986) Allozyme electrophoresis. A handbook of animal and population studies. Academic Press, San Diego
Ross GC (1976) Isoenzymes in Schistosoma spp.: LDH, MDH and acid phosphatases separated by isoelectric focusing in poly-acrylamide gel. Comp Biochem Physiol 55B:343–346
Ross GC (1977) Analysis by isoelectric focusing of phosphoglu-coisomerases in Schistosoma species and their snail hosts. Proc Anim Div Chem Soc 14:76–79
Ross GC, Southgate VR, Knowles RJ (1978) Observations on some isoenzymes of strains of Schistosoma bovis, S. mattheei, S. margrebowiei and S. leiperi. Z Parasitenkd 57:49–56
Shaw CR, Prasad R (1970) Starch gel electrophoresis of enzymes — a compilation of recipes. Biochem Genet 4:297–320
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Cutillas, C., Arias, P. & Spakulova, M. Malic dehydrogenase isoenzymatic pattern in lung-nematode parasite species. Parasitol Res 82, 92–94 (1996). https://doi.org/10.1007/s004360050076
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s004360050076