Abstract
In vitro leucocyte proliferative responses to parasite antigens and to mitogens as well as lymphocyte sub-types were compared in guinea pigs with genetically determined differences in their ability to express protective immunity against Trichostronylus colubriformis infection. Proliferative responses to parasite antigens were greatest in high-responder (HR) animals, but cells from low-responder (LR) animals were generally more responsive to mitogens. However, HR circulating leucocytes were more responsive to the T-cell-dependent B-cell mitogen pokeweed mitogen (PWM), and the response of HR, but not LR, cells increased during primary infection with T. colubriformis. Flow cytometry revealed significantly greater numbers of circulating B-cells in HR animals and, as observed for responsiveness to PWM, the number of circulating B-cells increased in HR, but not LR, animals during primary infection with this parasite. These findings suggest a larger and more labile population of B-cells in HR guinea pigs.
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Received: 16 August 1999 / Accepted: 21 October 1999
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Manjili, M., Sangster, N. & Rothwell, T. In vitro leucocyte proliferative responses and lymphocyte sub-types in guinea pigs with genetically determined high- and low-level responsiveness to Trichostrongylus colubriformis . Parasitol Res 86, 311–317 (2000). https://doi.org/10.1007/s004360050048
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DOI: https://doi.org/10.1007/s004360050048