Skip to main content

Advertisement

SpringerLink
Log in

Production of monoclonal antibodies using an electrophoretically separated diagnostic marker from the urine of individuals infected with Schistosoma japonicum

  • Original Paper
  • Published:
Parasitology Research Aims and scope Submit manuscript

Abstract.

In this paper, electrophoresis was successfully used to screen a diagnostic marker (a glycoprotein of 30 kDa) from the urine of individuals infected with Schistosoma japonicum, and then with the marker as antigen, two lines of monoclonal antibodies (mAbs) were obtained, NP56 and NP54. NP56 was of better immunoreactivity, its immunoglobulin isotype being IgG2b. NP56 reacted with soluble egg antigen and adult worm antigen and miracidia in eggs of S. japonicum. Using NP56 as a probe in indirect ELISA, the sensitivity and specificity (median egg excretion per gram of feces 69) was 90% and 100% in concentrated urine samples and 50% and 100% in original urine samples, respectively. The results indicate that the method is feasible for producing mAbs with diagnostic markers separated electrophoretically from the urine of individuals infected with pathogen.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Author information

Authors and Affiliations

  1. Department of Parasitology, Nanjing Medical University, Nanjing 210029, People’s Republic of China, People’s Republic of China

    Zhu Changliang, Tian Haisheng, Li Yunqian & Guan Xiaohong

  2. Clinical Examination Center, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, People’s Republic of China, People’s Republic of China

    Lu Lin

Authors
  1. Zhu Changliang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Tian Haisheng
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Lu Lin
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Li Yunqian
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Guan Xiaohong
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Electronic Publication

Rights and permissions

Reprints and permissions

About this article

Cite this article

Changliang, Z., Haisheng, T., Lin, L. et al. Production of monoclonal antibodies using an electrophoretically separated diagnostic marker from the urine of individuals infected with Schistosoma japonicum . Parasitol Res 88, 308–310 (2002). https://doi.org/10.1007/s004360000349

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s004360000349

Keywords

Search

Navigation