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Study on the genotypes of Echinococcus granulosus in yaks and sheep from Langkazi County in Tibet Autonomous Region of China based on mitochondrial cox1 and nad1

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Abstract

To determine the genotypes of the epidemic strains of Echinococcus granulosus in livestock in Tibet, samples of E. granulosus cysts were collected from 11 yaks and 62 sheep. Genomic DNA was extracted from these samples, and gene fragments of mitochondrial cytochrome c oxidase subunit I (cox1) and NADH dehydrogenase subunit I (nad1) were amplified by PCR and sequenced. DNASTAR and MAGA7.0 were employed for homology analysis and phylogenetic tree construction. Echinococcus granulosus cysts were detected in 56.2% (41/73) of the samples screened. Of these, 63.4% (26/41) were identified as E. granulosus G1 genotype (common sheep strain), 24.4% (10 /41) as G3 genotype (buffalo strain), and 12.2% (5/41) were G6 genotype (camel strain). The study concludes that yaks and sheep in Langkazi county, Tibet, carry three E. granulosus genotypes (G1, G3, and G6), with the G1 genotype the predominant genotype in the region. This study clarifies the distribution of E. granulosus genotypes, providing genetic data and insight for the surveillance and prevention of echinococcosis.

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Acknowledgements

This research received significant assistance from the Animal Disease Prevention and Control Centre in Shannan City, Tibet Autonomous Region, and the Animal Disease Prevention and Control Centre in Langkazi County, Shannan City. Our team expresses our gratitude for their support.

Funding

This research was supported by The Major Science and technology projects of Tibet (XZ202101ZD0002N) and the Program of Chinese National Beef and Yak Industry System (CARS-37).

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Author notes

  1. Zhonghua Su and Dongjing Wang contributed equally to this work.

Authors and Affiliations

  1. Tibet Autonomous Animal Disease Prevention and Control Center, Lhasa, 850000, Tibet, China

    Zhonghua Su

  2. Institute of Animal Husbandry and Veterinary, Tibet Autonomous Regional Academy of Agricultural Sciences, Lhasa, 850009, Tibet, China

    Dongjing Wang, Bin Shi & Wenqiang Tang

  3. Animal Science College, Tibet Agriculture & Animal Husbandry University, Nyingchi, 860000, Tibet, China

    Suolang Sizhu & Runbo Luo

  4. Department of Agriculture and rural Tibet, Lhasa, 850000, Tibet, China

    Qiming Wang

Authors
  1. Zhonghua Su
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  2. Dongjing Wang
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  4. Runbo Luo
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  5. Qiming Wang
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  6. Bin Shi
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  7. Wenqiang Tang
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Contributions

Conceptualization and study design: Zhonghua Su and Suolang Sizhu. Investigation and sample collection: Zhonghua Su and Dongjing Wang. Laboratorial analysis: Zhonghua Su, Dongjing Wang, Runbo Luo, and Bin Shi. Data analysis: Zhonghua Su and Dongjing Wang. Supervised study and secured funding for research: Suolang Sizhu and Wenqiang Tang. Writing—original draft: Zhonghua Su, Dongjing Wang, and Qiming wang. All authors reviewed the manuscript.

Corresponding author

Correspondence to Wenqiang Tang.

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Ethics approval

This study was conducted in accordance with the principles of the Declaration of Helsinki. All procedures performed in studies involving animals were in accordance with the ethical standards of the institution at which the studies were conducted, and all methodologies were supervised and authorized by the Tibet Autonomous Animal Disease Prevention and Control Center's Institutional Review Board.

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Su, Z., Wang, D., Sizhu, S. et al. Study on the genotypes of Echinococcus granulosus in yaks and sheep from Langkazi County in Tibet Autonomous Region of China based on mitochondrial cox1 and nad1. Parasitol Res 123, 132 (2024). https://doi.org/10.1007/s00436-024-08158-5

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