Abstract
Cystic echinococcosis (CE) is one of the most important helminthic diseases in the world with different genotypes distribution. The application of specific genotype antigens together with sera from patients with specific cyst genotypes have not been reported, so far. The present study aimed to apply and evaluate native AgB from Echinococcus granulosus sensu stricto (Eg) and Echinococcus canadensis (Ec) alone or mixture for serodiagnosis of human G1–G3 and G6/G7 genotypes cystic echinococcosis sera, using ELISA and Western blotting. A total of 47 human sera along with 47 human CE cysts were collected. CE genotypes were determined. Native AgB were prepared from E. granulosus s.s and E. canadensis genotypes. ELISA and Western blot were performed on human specific G1–G3 and G6/G7 genotypes sera. Species specific native AgB were used alone or mixed. The sensitivity of ELISA using alone and mixed 1Eg-1Ec, 1Eg-2Ec, and 2Eg-1Ec of native AgB from E. granulosus s.s and E. canadensis genotypes for human G1–G3 sera were 92.10, 89.47, 97.37, 100, and 100%, respectively; while using AgBs, alone and mixed for human G6/G7 sera were 100%. The sensitivity of Western blotting using native AgB of E. granulosus s.s and E. canadensis genotypes alone and mixed 2Eg-1Ec were 78.95% and 100% for human G1–G3 and G6/G7 genotypes sera, respectively. The mixture of AgB from Echinoccus granulosus sensu stricto and Echinococcus canadensis genotypes increased ELISA sensitivity for the diagnosis of human CE. Preparation and application of native AgB from specific and prevalent genotypes of CE in endemic regions is recommended.
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Data Availability
The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.
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Acknowledgements
The authors thank the staff of the surgery ward at the Abu Ali Sina Hospital for their cooperation, especially Ms. Nowrouzi and Ms. Mohammadi. The helps of Mrs. Sakineh Kazemian for her valuable assistance in the helminthology laboratory is acknowledged.
Funding
This project was financially supported by the office of vice-chancellor for research at Shiraz University of Medical Sciences, Grant No: 19339.
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Conceptualization, S.M.S.; methodology, Y.S.; validation, S.M.S and Y.S.; formal analysis, S.M.S.; writing—original draft preparation, Y.S., and S.M.S.; supervision, S.M.S.; ultrasonography by S.H.J; surgeries by H.N. and P.M.; a collection of samples by Y.S. and a part of the G6 samples by R.S.; project administration, S.M.S and Y.S.; funding acquisition, S.M.S. All authors have read and agreed to the published version of the manuscript.
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Sharifi, Y., Sadjjadi, S.M., Jafari, S.H. et al. Application and evaluation of native antigen B from Echinococcus granulosus sensu stricto and E. canadensis alone or mixture for serodiagnosis of human G1-G3 and G6/G7 genotypes cystic echinococcosis sera, using ELISA and Western blotting. Parasitol Res 122, 2227–2236 (2023). https://doi.org/10.1007/s00436-023-07924-1
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DOI: https://doi.org/10.1007/s00436-023-07924-1