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Morphological and molecular characterization of Hysterothylacium larval morphotypes (Nematoda: Raphidascarididae) infecting edible marine fish in the Black Sea

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Abstract

The morphological and molecular identification of Hysterothylacium larval morphotypes in the Black Sea remains unresolved and incomplete. The aim of current study was to provide a detailed morphological identification with rDNA whole ITS (ITS‒1, 5.8S subunit, ITS‒2) and mtDNA cox2 sequences data of Hysterothylacium larval morphotypes infecting four commonly edible marine fish species, including European anchovy, Engraulis encrasicolus (L.), horse mackerel, Trachurus trachurus (L.), whiting, Merlangius merlangus (L.), and red mullet, Mullus barbatus ponticus (E.) in the Black Sea (FAO fishing area 37.4.2). Hysterothylacium larval morphotypes were morphologically classified, followed by whole ITS and cox2 sequencing. Four Hysterothylacium larval morphotypes, III, IV, VIII, and IX, are described based on morphological and molecular data. The present study provides the first study reporting whole ITS and cox2 sequences for Hysterothylacium larval morphotypes III, IV, VIII and III, IV, VIII, IX, respectively, in the Black Sea. Here, we offer a foundation for future research on the distribution, morphologic and molecular identification of Hysterothylacium larval morphotypes infecting edible some marine fish in the Black Sea.

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Data availability

Data supporting the conclusions of this article are included within the article. Nucleotide sequences generated in this study were deposited in the GenBank database under the accession numbers MZ313180‒MZ313183 and MZ313477‒ MZ313488.

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Funding

This study was supported by the Scientific Research Council of Ondokuz Mayis University, Samsun, Türkiye [grant number PYO.VET.1904.20.022].

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Authors and Affiliations

  1. Samsun Directorate of Provincial Agriculture and Forestry, Republic of Türkiye Ministry of Agriculture and Forestry, 55200, Samsun, Türkiye

    Muhammet Yavuz Gelen

  2. Department of Aquatic Animal Diseases, Faculty of Veterinary Medicine, Ondokuz Mayis University, 55200, Samsun, Türkiye

    Gokmen Zafer Pekmezci

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  1. Muhammet Yavuz Gelen
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  2. Gokmen Zafer Pekmezci
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Contributions

G.Z.P. supervised and coordinated the present study. G.Z.P. came up with the study idea and drafted the initial proposal. G.Z.P. and M.Y.G. carried out the laboratory analyses. G.Z.P. and M.Y.G. have also read and approved the final manuscript. This work is also summarized from the master thesis of M.Y.G., Ondokuz Mayis University, Samsun, Türkiye, in 2021.

Corresponding author

Correspondence to Gokmen Zafer Pekmezci.

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Gelen, M.Y., Pekmezci, G.Z. Morphological and molecular characterization of Hysterothylacium larval morphotypes (Nematoda: Raphidascarididae) infecting edible marine fish in the Black Sea. Parasitol Res 122, 1863–1872 (2023). https://doi.org/10.1007/s00436-023-07887-3

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