Abstract
The studies of opisthorchiids larval stages associated with Bithyniidae snails can provide important and the most reliable data for opisthorchiidoses foci characterization due to the low mobility of such opisthorchiid’s host species as Bithyniidae snails. The foci of opisthorchiosis (caused by Opisthorchis felineus) and metorchiosis (caused by Metorchis bilis) are overlapping in the basins of the Ob and Irtysh Rivers. Thus, difficulties with determining the species of cercariae significantly reduce the accuracy of epidemiological conclusions regarding opisthorchiosis, which has a much higher medical significance. Moreover, M. bilis cercariae identification is complicated by the fact that the focus of metorchiosis caused by Metorchis xanthosomus (infecting fish-eating predatory birds) occurs on the same territory. In this study, we for the first time carried out the molecular genetic identification of West-Siberian opisthorchiid cercariae to verify morphological identification and confirmed O. felineus’s main association with Bithynia troschelii snail and M. bilis’—with B. tentaculata snail. Thus, our study applied a two-stage approach: the morphological identification of any opisthorchiid-like cercariae in Bithynia snails and subsequent molecular genetic precise species determination of cercariae in the reduced samples.
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Data availability
The sequences generated in the study have been deposited in the GenBank database under the accession number MK517653 (O. felineus isolate) and MK517652 (M. bilis isolate).
Code availability
Not applicable.
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Acknowledgements
We would like to thank Dr. D. Petrovski for the assistance with statistical analysis; Dr. I.M. Malykh for the help with molecular genetic analysis of cercariae samples, and all researchers of the Chany Field Station for their help with fieldwork.
Funding
Morphology and ecology part of this work was funded by the Research Program of ISEA SB RAS for 21 2021–2025, project number: 122011800267–4, and molecular part—by the Research Programme of ICG SB RAS, project number: FWNR-2022–0019.
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AVK: conceptualization, methodology, molecular genetic analysis, writing original draft. EAS: conceptualization, methodology, morphology and ecology analysis, writing original draft. Both authors read and approved the final manuscript.
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Katokhin, A.V., Serbina, E.A. Molecular genetic species definition of Opisthorchiidae-like cercariae from Bithyniidae snails of Chany Lake (South-Western Siberia, Russia). Parasitol Res 122, 341–345 (2023). https://doi.org/10.1007/s00436-022-07725-y
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DOI: https://doi.org/10.1007/s00436-022-07725-y