Abstract
Immature trematodes of Lecithochirium sp. are recorded for the first time as parasites of the California two-spot octopus Octopus bimaculatus from Bahía de los Ángeles, Baja California, Mexico. Thirty-nine O. bimaculatus were examined for trematodes and a total of 100 immature specimens of Lecithochirium sp. were recorded from the crop of seven infected octopuses. Based on these records, O. bimaculatus may act as a second intermediate or paratenic host for these parasites. Partial sequences of the 28S (region D1-D3) ribosomal gene corroborate the identifications based on morphological characters. DNA sequences of the 28S gene from GenBank were analyzed to include the immature samples of Lecithochirium sp. within a hemiurid phylogenetic framework. All immature specimens of Lecithochirium sp. were recovered as monophyletic and Pulmovermis cyanovitellosus was identified as the sister species of Lecithochirium sp. However, due to the lack of molecular data for species of the genus Lecithochirium, these phylogenetic inferences must be taken with caution. Therefore, the morphological and molecular data obtained here provide a foundation for future work to develop a systematic comparison among- and within-species of the genus Lecithochirium. Additionally, the present records of Lecithochirium in O. bimaculus add to the knowledge of the parasite fauna of cephalopods.
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Sequence data has been submitted to GenBank.
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Acknowledgements
We are grateful to Arturo Rincón Sandoval (Ms. Sc. student, Maestría y Doctorado en Ciencias e Ingeniería [MyDCI], Facultad de Ciencias, Universidad Autónoma de Baja California [UABC], Mexico) for technical support with the microscopy equipment. We are grateful to Abril Gamboa and David Hernández-Mena for technical assistance in the molecular lab (CINVESTAV-IPN, Merida, Yucatán, Mexico). Thanks to Graciela E. Colunga-Ramírez, Jesus Galarza, and Jorge Sánchez-Solís for their help during fieldwork. AJCM was supported by a CONACyT scholarship (No. 712235) to accomplish his Ms. Sc. degree. We also thank Dr. Fadia Sara Ceccarelli of the Center for Scientific Research and Higher Education at Ensenada, Mexico (CICESE) who reviewed the first draft of this manuscript and made very useful suggestions of the phylogenetic analyses that we present in this contribution. We are grateful to Sean M. Rovito for help with the language editing and for his constructive criticism. The manuscript greatly benefited from comments of two anonymous referees.
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This work was financially supported from Grant No. 286347 by the Consejo Nacional de Ciencia y Tecnología (CONACyT) to SCM, partially funded by the Grant No. 201441 (CONACyT, Mexican Ministry of Energy, Hydrocarbon Trust) to MLAM, and also partially funded by the Grant No. 400/2356 (Programa para el Desarrollo Profesional Docente [UABC–PRODEP–2019] from Secretaría de Educación Pública [SEP], Mexico) to AMA.
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AJCM collected samples in the laboratory, mounted and made the preliminarily identification of the specimens, produced the molecular data, carried out the formal analysis, and prepared the figures and tables. SCM conceived the study, made the field observations, acquired funding and contributed reagents/materials/analysis tools. MLAM contributed reagents/materials/analysis tools, identified the specimens, and acquired funding. AMA conceived the research, acquired funding, identified the specimens, prepared figures and tables, and carried out the formal analysis, data curation, visualization, writing—original draft, writing—review and editing. All authors reviewed the manuscript.
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Chan-Martin, A.d., Castellanos-Martínez, S., Aguirre-Macedo, M.L. et al. Immature trematodes of Lecithochirium sp. (Digenea: Hemiuridae) in the California two-spot octopus (Octopus bimaculatus) from Mexico. Parasitol Res 121, 2651–2660 (2022). https://doi.org/10.1007/s00436-022-07590-9
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DOI: https://doi.org/10.1007/s00436-022-07590-9