Abstract
The aim of the present study was to determine the effective dose of fosfatriclaben through a field study in sheep naturally infected with F. hepatica. Thirty crossbred sheep positive for fluke eggs were selected for inclusion in the trial. On day 0, 5 groups of 6 animals each were formed for treatments: group 1 (G1), G2, and G3 received fosfatriclaben at 4, 6, and 8 mg/kg/IM, respectively. G4 received triclabendazole at 10 mg/kg/PO, and G5 was the untreated control group. Fecal samples of the sheep were analyzed to count the number of fluke eggs to evaluate the percentage of egg reduction. Twenty-one days after treatment, all sheep were humanely euthanized to extract the flukes from the bile ducts. They were counted to assess the percentage of fluke reduction. Fosfatriclaben reduced fluke eggs by 99.6, 99.6, and 100% and flukes by 94.3, 100, and 100%, respectively. Triclabendazole reduced fluke eggs by 95.2% and flukes by 100%. The fosfatriclaben injectable prodrug showed a high fasciolicidal efficacy similar to triclabendazole, with advantages over its predecessor, since only half the dose as compared to triclabendazole was required to remove eggs and flukes in the sheep that were studied.
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Acknowledgements
Grateful acknowledgement for assistance is due to Dr. Ignacio Olave-Leyva from Facultad Veterinaria de Tulancingo, Hgo., Gerardo Francisco-Márquez from Facultad de Medicina Veterinaria y Zootecnia – UNAM. M. Flores-Ramos is very grateful to Dirección General de Asuntos del Personal Académico (DGAPA-UNAM) for the Postdoctoral fellowship granted.
Funding
This work was supported by the project PAPIIT, DGAPA, UNAM-IT201018.
All procedures performed in this work are in agreement with the ethical standards of institutional guidelines on the care and use of animals.
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Ibarra-Velarde, F., Rojas-Campos, T., Vera-Montenegro, Y. et al. Field study on the determination of the effective dose of injectable fosfatriclaben prodrug in sheep naturally infected with Fasciola hepatica. Parasitol Res 121, 433–440 (2022). https://doi.org/10.1007/s00436-021-07366-7
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DOI: https://doi.org/10.1007/s00436-021-07366-7