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Supplementary morphological data and molecular analyses of Eimeria labbeana (Labbé, 1896) Pinto, 1928 (Chromista: Miozoa: Eimeriidae) from columbiform birds in Portugal

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Abstract

Columbiformes have a worldwide distribution, of which 166 species occur in Eurasia. They have been reported parasitized by coccidians recurrently in recent years; however, Eimeria labbeana (Labbé, 1896) Pinto, 1928, which is first Eimeria sp. from Columbiformes described in the late nineteenth century, is not taxonomically identified by its oocysts since the 1930s. In this context, the current study aimed to supplement the morphology of E. labbeana from Eurasian collared doves Streptopelia decaocto Frivaldszky, 1838 and from a common woodpigeon Columba palumbus Linnaeus, 1758 in Portugal, providing a preliminary genotypic characterization. Three of the four columbiforms were positive for oocysts identified as E. labbeana, which were morphologically revised as having micropyles, in addition to other minor adjustments. Oocysts from S. decaocto and C. palumbus were morphologically identical and equivalent in all morphometric aspects, besides having genotypic similarity of 99.5%. Phylogenetic analysis based on the mitochondrial cytochrome c oxidase subunit 1 gene resulted in a large clade with Eimeria spp. and Isospora spp. from different vertebrates and low similarity between Eimeria spp. from Columbiformes, whereas the phylogenetic analysis based on the small subunit ribosomal RNA gene resulted in well-supported monophyletic groups, including one with the coccidians of columbiform birds.

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Acknowledgements

We thank the staff at the Lisbon Center for Wild Animal Recovery, in Monsanto Forest Park, Lisbon, Portugal, who enabled the collection of samples from birds held for rehabilitation and reintroduction into the wild.

Funding

This study was supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ), and Centro de Investigação Interdisciplinar em Sanidade Animal (Project UIDP/CVT/00276/2020, funded by FCT). MSO have scholarship from CAPES (Grant/Award Number: 001). BPB has a fellowship from CNPq (Grant/Award Number: 303899/2019–0) and from FAPERJ (Grant/Award Number: E-26/202.797/2019). DWR has a postdoctoral fellowship from FCT (Grant/Award Number: SFRH/BPD/115202/2016).

Author information

Authors and Affiliations

  1. Programa de Pós-Graduação em Biologia Animal, Instituto de Ciências Biológicas e da Saúde (ICBS), Universidade Federal Rural do Rio de Janeiro (UFRRJ), BR-465 km 7, Seropédica, RJ, 23897-000, Brazil

    Mariana S. Oliveira

  2. Centro de Investigação Interdisciplinar em Sanidade Animal (CIISA), Faculdade de Medicina Veterinária, Universidade de Lisboa, Avenida da Universidade Técnica, 1300-477, Lisboa, Portugal

    David Wilson Ramilo & Isabel P. Fonseca

  3. Faculdade de Medicina Veterinária, Universidade Lusófona de Humanidades e Tecnologias, Campo Grande 376, 1749-024, Lisboa, Portugal

    David Wilson Ramilo

  4. Programa de Pós-Graduação em Ciências Veterinárias, Instituto de Veterinária, UFRRJ, BR-465 km 7, Seropédica, RJ, 23897-000, Brazil

    Ericson R. Mello

  5. Programa de Pós-Graduação em Biomedicina Translacional, Universidade do Grande Rio, Rua Professor José de Souza Herdy 1160, Duque de Caxias, RJ, 25071-202, Brazil

    Sergian V. Cardozo

  6. Centro de Reabilitação de Animais Selvagens de Lisboa (LxCRAS), Parque Florestal de Monsanto, Estrada do Barcal, 1500-068, Lisboa, Portugal

    Inês Caetano & Erica Brazio

  7. Departamento de Microbiologia e Imunologia Veterinária, IV, UFRRJ, BR-465 km 7, Seropédica, RJ, 23897-000, Brazil

    Águida A. Oliveira

  8. Departamento de Biologia Animal, ICBS, UFRRJ, BR-465 km 7, Seropédica, RJ, 23897-000, Brazil

    Viviane M. Lima & Bruno P. Berto

Authors
  1. Mariana S. Oliveira
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  2. David Wilson Ramilo
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  3. Ericson R. Mello
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  4. Sergian V. Cardozo
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  5. Inês Caetano
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  6. Erica Brazio
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  7. Isabel P. Fonseca
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  8. Águida A. Oliveira
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  9. Viviane M. Lima
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  10. Bruno P. Berto
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Corresponding author

Correspondence to Bruno P. Berto.

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All applicable institutional, national and international guidelines for the care and use of animals were followed. Animal manipulation in the Lisbon Center for Wild Animal Recovery was performed by suitably qualified professionals, according to the directive 86/609/EEC.

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The authors declare no competing interests.

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Oliveira, M.S., Ramilo, D.W., Mello, E.R. et al. Supplementary morphological data and molecular analyses of Eimeria labbeana (Labbé, 1896) Pinto, 1928 (Chromista: Miozoa: Eimeriidae) from columbiform birds in Portugal. Parasitol Res 120, 3569–3580 (2021). https://doi.org/10.1007/s00436-021-07300-x

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