Abstract
An isolated population of several hundred Iberian ibex (Capra pyrenaica) inhabits the Gerês-Xurés Transboundary Biosphere Reserve (GXTBR) in north-western Iberian Peninsula, in partial sympatry with tens of thousands domestic goats (Capra hircus). This study aimed to assess the prevalence and shedding intensity of gastrointestinal parasites from sympatric herds of domestic goat and Iberian ibex in autumn 2018. A total of 93 pooled faecal samples were collected from GXTBR (39 from domestic goats and 54 from Iberian ibex) and the host species was identified using molecular techniques, whenever defecation was not visualised in the field. Parasitological analysis was achieved by joint Willis flotation/sedimentation, McMaster and Mini-FLOTAC techniques. Seventy-two samples (25 domestic goats and 47 Iberian ibex) were retained for analysis after discarding the least fresh and those with uncertain specific identification. Generalized linear mixed models compared prevalence and shedding intensity between caprine species. Domestic goats showed a non-significant tendency to be more parasitized than Iberian ibex, as assessed by overall prevalence (100.0%, CI95 86.7–100.0 vs. 74.5%, CI95 69.5–84.8), and shedding intensity. This study reveals a similar community of gastrointestinal parasites in an abundant livestock species and an isolated population of wild caprine, living in partial sympatry. It is the first study on the health status of this endangered Iberian ibex population, in close contact with livestock, highlighting the need for further sanitary surveillance.
References
Alasaad S, Morrondo P, Dacal-Rivas V, Soriguer RC, Granados JE, Serrano E, Zhu XQ, Rossi L, Pérez JM (2009) Bronchopulmonary nematode infection of Capra pyrenaica in the Sierra Nevada massif, Spain. Vet Parasitol 164:340–343. https://doi.org/10.1016/j.vetpar.2009.06.019
Apio A, Mohammed OB, Omer SA, Wronski T (2013) Cross infection with gastro-intestinal tract parasites between domestic goat and endemic Farasan gazelle (Gazella gazella farasani) in Farasan Islands, Saudi Arabia. J King Saud Univ - Sci 25:325–329. https://doi.org/10.1016/j.jksus.2013.02.003
Benayas J, Scheiner S (2002) Plant diversity, biogeography and environment in Iberia: patterns and possible causal factors. J Veg Sci 13:245–258. https://doi.org/10.1111/j.1654-1103.2002.tb02045.x
Cabral MJ (c), Almeida J, Almeida PR, Delliger T, Ferrand de Almeida N, Oliveira ME, Palmeirim JM, Queirós AI, Rogado L, Santos-Reis M (eds) (2005) Livro Vermelho dos Vertebrados de Portugal. Instituto da Conservação da Natureza, Lisboa, 659 pp
Calero-Bernal R, García-Moreno A, González-Barrio D, Nieto-Rodríguez JM, Fernández-Llario P, de Mendoza JH, Habela MÁ (2020) Infectious diseases surveillance of the Iberian ibex (Capra pyrenaica victoriae) in Western Spain: health and conservation implications. Galemys 32:13–20. https://doi.org/10.7325/Galemys.2020.A2
Cassinello J, Acevedo P (2007) Capra pyrenaica Schinz, 1838. In: Palomo LJ, Gisbert J, Blanco JC (eds) Atlas y libro rojo de los mamíferos de España. Dirección General para la Biodiversidad-SECEM -SECEMU, Madrid, pp 369–370
Cassini R, Párraga MA, Signorini M, Frangipane Di Regalbono A, Sturaro E, Rossi L, Ramanzin M (2015) Lungworms in Alpine ibex Capra ibex in the eastern Alps, Italy: an ecological approach. Vet Parasitol 214:132–138. https://doi.org/10.1016/j.vetpar.2015.09.026
Cringoli G, Maurelli MP, Levecke B, Bosco A, Vercruysse J, Utzinger J, Rinaldi L (2017) The Mini-FLOTAC technique for the diagnosis of helminth and protozoan infections in humans and animals. Nat Protoc 12:1723–1732. https://doi.org/10.1038/nprot.2017.067
De Castro F, Bolker B (2005) Mechanisms of disease-induced extinction. Ecol Lett 8:117–126. https://doi.org/10.1111/j.1461-0248.2004.00693.x
Fajardo V, González I, Dooley J, Garret S, Brown HM, García T, Martín R (2009) Application of polymerase chain reaction-restriction fragment length polymorphism analysis and lab-on-a-chip capillary electrophoresis for the specific identification of game and domestic meats. J Sci Food Agric 89:843–847. https://doi.org/10.1002/jsfa.3522
Figueiredo A, Oliveira L, de Carvalho LM, Fonseca C, Torres RT (2016) Parasite species of the endangered Iberian wolf (Canis lupus signatus) and a sympatric widespread carnivore. Int J Parasitol Parasites Wildl 5(2):164–167. https://doi.org/10.1016/j.ijppaw.2016.04.002
Fonseca C, Migueis D, Fernandes T, Carvalho H, Loureiro A, Carvalho J, Torres RT (2017) The return of the Iberian wild goat Capra pyrenaica to Portugal: from reintroduction to recolonization. J Nat Conserv 38:56–61. https://doi.org/10.1016/j.jnc.2017.05.006
Foreyt WJ (2001) Veterinary parasitology, 5th edn. Iowa State University Press, Iowa
Gandra F, Vila-Viçosa M, Cortes H, Araújo J, Mateus T (2016) Goat production in the county of Terras de Bouro: forest fire prevention, organic goat production and parasitism. In: Olsson IAS, Araújo SM, Vieira MF (eds) Food futures: ethics, science and culture. Wageningen Academic Publishers, Wageningen, pp 159–173
Hudson PJ, Cattadori IM, Boag B, Dobson AP (2006) Climate disruption and parasite–host dynamics: patterns and processes associated with warming and the frequency of extreme climatic events. J Helminthol 80:175–182. https://doi.org/10.1079/joh2006357
INE (2020a) Statistics Portugal. Instituto Nacional de Estatística. https://www.ine.pt/xportal. Accessed 21 Jan 2020
INE (2020b) INEBase. Instituto Nacional de Estadística https://www.ine.es/dyngs/INEbase/es. Accessed 21 Jan 2020
Jacobs D, Fox M, Gibbons L, Hermosilla C (2016) Principles of veterinary parasitology. John Wiley & Sons, Ltd, Chichester, West Sussex, UK
Luzón M, Santiago-Moreno J, Meana A, Toledano-Díaz A, Pulido-Pastor A, Gómez-Brunet A, López-Sebastián A (2008) Parasitism and horn quality in male Spanish ibex (Capra pyrenaica hispanica) from Andalucía based on coprological analysis and muscle biopsy. Spanish J Agr Res 6:353–361. https://doi.org/10.5424/sjar/2008063-328
MAFF (1986) Manual of Veterinary Parasitological Laboratory Techniques, 3rd edn. H.M.S.O, London
Moço G, Guerreiro M, Ferreira AF, Rebelo A, Loureiro A, Petrucci-Fonseca F, Pérez JM (2006) The ibex Capra pyrenaica returns to its former Portuguese range. Oryx 40:351–354. https://doi.org/10.1017/S0030605306000718
Moço G, Serrano E, Guerreiro M, Ferreira AF, Petrucci-Fonseca F, Santana D, Maia MJ, Soriguer RC, Pérez JM (2014) Does livestock influence the diet of Iberian ibex Capra pyrenaica in the Peneda-Gerês National Park (Portugal)? Mammalia 78:393–399. https://doi.org/10.1515/mammalia-2013-0139
Monterroso P, Godinho R, Oliveira T, Ferreras P, Kelly MJ, Morin DJ, Waits LP, Alves PC, Mills LS (2019) Feeding ecological knowledge: the underutilised power of faecal DNA approaches for carnivore diet analysis. Mammal Rev 49:97–112. https://doi.org/10.1111/mam.12144
Pérez JM, Granados JE, Soriguer RC, Fandos P, Márquez FJ, Crampe JP (2002) Distribution, status and conservation problems of the Spanish Ibex, Capra pyrenaica (Mammalia: Artiodactyla). Mammal Rev 32:26–39. https://doi.org/10.1046/j.1365-2907.2002.00097.x
Pérez JM, Granados JE, Pérez MC, Márquez FJ, Ferroglio E, Rossi L (2003) A survey of the gastrointestinal nematodes of Spanish ibex (Capra pyrenaica) in a high mountain habitat. J Parasitol 89:315–318. https://doi.org/10.1645/0022-3395(2003)089[0315:ASOTGN]2.0.CO;2
R Development Core Team (2017). R, A language and environment for statistical computing. R Foundation for Statistical Computing
Richomme C, Gauthier D, Fromont E (2006) Contact rates and exposure to inter-species disease transmission in mountain ungulates. Epidemiol Infect 134:21–30. https://doi.org/10.1017/S0950268805004693
Santiago-Moreno J, Luzón M, Coloma MA, Pulido-Pastor A, Gómez-Guillamón F, de la Vega RS, Toledano-Díaz A, López-Sebastián A (2010) Relationship between Iberian ibex (Capra pyrenaica) sperm quality and level of parasitism. Eur J Wildl Res 56:605–611. https://doi.org/10.1007/s10344-009-0356-2
Van Wyk JA, Cabaret J, Michael LM (2004) Morphological identification of nematode larvae of small ruminants and cattle simplified. Vet Parasitol 119:277–306. https://doi.org/10.1016/j.vetpar.2003.11.012
Walker JG, Morgan ER (2014) Generalists at the interface: nematode transmission between wild and domestic ungulates. Int J Parasitol Parasites Wildl 3:242–250. https://doi.org/10.1016/j.ijppaw.2014.08.001
Willis HH (1921) A simple levitation method for the detection of hookworm ova. Med J Aust 2(18):375–376. https://doi.org/10.5694/j.1326-5377.1921.tb60654.x
Zhang L, Yang X, Wu H, Gu X, Hu Y, Wei F (2011) The parasites of giant pandas: individual-based measurement in wild animals. J Wildl Dis 47:164–171. https://doi.org/10.7589/0090-3558-47.1.164
Acknowledgements
Fundação para a Ciência e a Tecnologia (FCT) supported the post-doctoral fellowship of N. Santos (SFRH/BPD/116596/2016). The authors acknowledge the support from Direção Regional de Conservação da Natureza e Florestas do Norte, Instituto de Conservação da Natureza e Florestas (ICNF).
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This research was funded by Centro de Investigação Interdisciplinar em Sanidade Animal (CIISA) through Project UIDB/00276/2020 funded by Fundação para a Ciência e a Tecnologia (FCT).
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Cardoso, B., Pessoa, B., Figueiredo, P. et al. Comparative survey of gastrointestinal parasites in sympatric Iberian ibex (Capra pyrenaica) and domestic goats using molecular host specific identification. Parasitol Res 120, 2291–2296 (2021). https://doi.org/10.1007/s00436-021-07174-z
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DOI: https://doi.org/10.1007/s00436-021-07174-z