Abstract
Trichinella pseudospiralis is a non-encapsulated species infecting both mammals and birds. In Italy, this species has been reported so far only in central regions (two nocturnal birds of prey, one red fox, and one wild boar) and in northeast regions (four wild boars). In November 2020, Trichinella sp. larvae were isolated by enzymatic digestion from muscle tissues of a red kite (Milvus milvus) specimen belonging to a population residing in the Basilicata region (Southern Italy). The parasite was identified as T. pseudospiralis by multiplex PCR, and the sequencing of the expansion segment V (ESV) region of the nuclear large subunit ribosomal DNA showed, in the microsatellite region, the polymorphism characteristic of the Palearctic population. This represents the first record of T. pseudospiralis in a red kite and the first report of this parasite in Southern Italy. The isolation of the parasite in a resident bird confirms that T. pseudospiralis is present, even if at low prevalence, in the Italian avifauna.
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References
Allavena S, Andreotti A, Angelini J, Scotti M (eds) (2006). In: Status e Conservazione del Nibbio reale Milvus milvus e del Nibbio bruno Milvus migrans in Italia e in Europa meridionale. Serra S. Quirico (AN): 4-5
BirdLife International (2021) Species factsheet: Milvus milvus. Downloaded from http://www.birdlife.org on 13/01/2021
Caruso M, Romano AC, Toce M, Martoccia R, Fraccalvieri R, Disanto N, Santagada G, Palazzo L (2019) Monitoraggio della Trichinellosi nella Fauna Selvatica in Basilicata. In: Atti XIX Congresso S.I.Di.L.V. Matera: 121-122
Cillo N, Laterza M (2014) Il nibbio reale in Italia. In: Life project Save the flyers “La conservazione del nibbio reale in Europa”. Santa Fiora (GR) 5-7 November 2014
Davis PE, Davis JE (1981) The food of the Red Kite in Wales. Bird Study 28:33–40
ISO 18743:2015, Microbiology of the food chain — detection of Trichinella larvae in meat by artificial digestion method. First edition 2015-09-15, International Organization for Standardization, Geneva, Switzerland
Mancianti F, Terracciano G, Sorichetti C, Vecchio G, Scarselli D, Perrucci S (2020) Epidemiologic survey on Toxoplasma gondii and Trichinella pseudospiralis infection in corvids from Central Italy. Pathogens 9(5):336
Garkavi BL (1972) Species of Trichinella isolated from wild animals. Veterinariya 10:90–91
La Rosa G, Marucci G, Zarlenga DS, Pozio E (2001) Trichinella pseudospiralis populations of the Palearctic region and their relationship with populations of the Nearctic and Australian regions. Int J Parasitol 31:297–305
Pozio E, Goffredo M, Fico R, La Rosa G (1999) Trichinella pseudospiralis in sedentary night-birds of prey from Central Italy. J Parasitol 85:759–761
Pozio E, Marucci G, Casulli A, Sacchi L, Mukaratirwa S, Foggin CM, La Rosa G (2004) Trichinella papuae and Trichinella zimbabwensis induced infection in experimentally infected varans, caimans, pythons and turtles. Parasitology 128:333–342
Pozio E, La Rosa G (2010) Trichinella. In: Liu D (ed) Molecular Detection of Foodborne Pathogens. CRC Press, Taylor & Francis Group, Boca Raton
Pozio E (2016) Trichinella pseudospiralis an elusive nematode. Vet Parasitol 231:97–101
Pozio E (2019) Trichinella and Trichinellosis In Europe. Vet Glas 00:1–20
Sigismondi A, Cillo N, Laterza M (2006) Status del Nibbio reale e del Nibbio bruno in Basilicata. In: Andreotti A, Angelini J, Scotti M (eds) Allavena S. Status e Conservazione del Nibbio reale Milvus milvus e del Nibbio bruno Milvus migrans in Italia e in Europa meridionale. Serra S, Quirico (AN), pp 26–27
Wildman L, O'Toole L, Summers RW (1998) The diet and foraging behaviour of the Red Kite in northern Scotland. Scot Birds 19:134–140
Zarlenga DS, Chute MB, Martin A, Kapel CM (1999) A multiplex PCR for unequivocal differentiation of all encapsulated and non-encapsulated genotypes of Trichinella. Int J Parasitol 29(11):1859–1867
Funding
Funding was provided by the Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata, by European Commission and by Istituto Superiore di Sanità. This study was supported in part by the European Commission, contract SANCO/2006/FOOD SAFETY/032.
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Marucci, G., Romano, A.C., Interisano, M. et al. Trichinella pseudospiralis in a red kite (Milvus milvus) from Italy. Parasitol Res 120, 2287–2290 (2021). https://doi.org/10.1007/s00436-021-07165-0
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DOI: https://doi.org/10.1007/s00436-021-07165-0