Skip to main content
Log in

Prevalence and molecular identification of Leucocytozoon spp. in fighting cocks (Gallus gallus) in Thailand

  • Protozoology - Original Paper
  • Published:
Parasitology Research Aims and scope Submit manuscript

Abstract

Leucocytozoon has been globally described as avian hosts, and it significantly affects many avian taxa including the fighting cock. The clinical signs of leucocytozoonosis range from asymptomatic to high morbidity leading to increase mortality rates. Interestingly, there are insufficient epidemiological studies of this blood parasite or the molecular identification of infections within the vertebrate host. In this study, 250 blood samples were collected from fighting cocks at 9 districts in Maha Sarakham Province, Thailand. Leucocytozoon infections were screened by blood examination and nested PCR followed by sequence analysis of mitochondrial cytochrome b gene was used to identify Leucocytozoon species. Twenty-two out of 250 (8.8%) samples had confirmed Leucocytozoon infections based on microscopic examination whereas with nested-PCR, 52 samples tested positive. Of these 52, 51 were successfully sequenced among which, one was Plasmodium juxtanucleare, 45 were Leucocytozoon sp. (18%) and 5 were L. schoutedeni (2%). This study is the first report to demonstrate the molecular prevalence of leucocytozoonosis in fighting cock in Thailand. This study indicated that leucocytozoonosis is widespread in fighting cock although the frequency was not determined and needs further study.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

Download references

Acknowledgements

This research project was financially supported by Mahasarakham University (2020).

Author information

Authors and Affiliations

Authors

Contributions

Supawadee Piratae: Conceptualization, Funding acquisition, Data curation, Formal analysis, Investigation, Methodology, Project administration, Writing - review and editing. Kotchaphon Vaisusuk: Conceptualization, Investigation, Methodology. Wasupon Chatan: Investigation

Corresponding author

Correspondence to Supawadee Piratae.

Ethics declarations

Ethics statement

All steps for animal handling and blood collections were conducted by veterinarians, and all fighting cocks were approved from their owners. All experimental procedures involving animals were conducted and approved by the Institutional Animal Care and Use Committee, Mahasarakham University (IACUC-MSU-006/2020).

Conflict of interest

The authors declare that they have no conflict of interest.

Additional information

Section Editor: Leonhard Schnittger

Publisher’s note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Piratae, S., Vaisusuk, K. & Chatan, W. Prevalence and molecular identification of Leucocytozoon spp. in fighting cocks (Gallus gallus) in Thailand. Parasitol Res 120, 2149–2155 (2021). https://doi.org/10.1007/s00436-021-07131-w

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00436-021-07131-w

Key words

Navigation