Abstract
The aim of this experiment was to determine the yield of Haemonchus contortus third-stage larvae (L3) in faecal cultures in different conditions, including incubation time (7 or 14 days), the addition of inert additives (polystyrene pellets, vermiculite or no additive) and physical condition of the incubated faeces (ground or whole pellets). Twelve groups of 10 cultures each were arranged and incubated at 24 °C to evaluate the interaction of the above-mentioned conditions. Significantly, more L3 (p=0.0019 to p=0.0200) were recovered from cultures incubated for 7 days than for 14 days, except for the groups containing whole pellets with no additives (p=0.53) or with vermiculite (p=0.41). Larval yields from 7-day incubated cultures did not differ between groups (p=0.47), but for the whole pellets with vermiculite group, which yielded significantly less L3 (p<0.0001) than the rest of the cultures. Incubation for 14 days showed that cultures containing whole pellets with no additives yielded significantly more L3 (p<0.05) than the rest. Culturing faeces with H. contortus seems not to require inert additives or extra manipulation to obtain good L3 yields.
This is a preview of subscription content, log in via an institution to check access.
References
Agyei AD (1995) An improved method of using sawdust as a faecal culture medium for the production of 3rd stage strongylate nematode larvae. Ghana J Agric Sci 28–29:11–15. https://doi.org/10.4314/gjas.v28i1.2002
Baermann G (1917) Eine einfache Methode zur Auffinding von Ankylostomum (Nematoden) Larven in Erdproben. Geneeskd Tidjschrift voor Ned Indie 57:131–137
Berrie DA, East IJ, Bourne AS, Bremner KC (1988) Differential recoveries from faecal cultures of larvae of some gastro-intestinal nematodes of cattle. J Helminthol 62:110–114. https://doi.org/10.1017/S0022149X00011330
Brunsdon RV (1968) Trichostrongyle worm infection in cattle: Ostertagiasis—Effect of a field outbreak on production, with a review of the disease syndromes, problems of diagnosis and treatment. N Z Vet J 16:176–187
Chylinski C, Cortet J, Sallé G, Jacquiet P, Cabaret J (2015) Storage of gastrointestinal nematode infective larvae for species preservation and experimental infections. Parasitol Res 114:715–720. https://doi.org/10.1007/s00436-014-4238-5
Coles GC, Bauer C, Borgsteede FHM, Geerts S, Klei TR, Taylor MA, Waller PJ (1992) World Association for the Advancement of Veterinary Parasitology (W.A.A.V.P.) methods for the detection of anthelmintic resistance in nematodes of veterinary importance. Vet Parasitol 44:35–44. https://doi.org/10.1016/0304-4017(92)90141-U
Coles GC, Jackson F, Pomroy WE, Prichard RK, von Samson-Himmelstjerna G, Silvestre A, Taylor MA, Vercruysse J (2006) The detection of anthelmintic resistance in nematodes of veterinary importance. Vet Parasitol 136:167–185. https://doi.org/10.1016/j.vetpar.2005.11.019
Corticelli B, Lai M (1963) Studies on the technique of culture of infective larvae of gastrointestinal strongyles of cattle. Acta Med Vet (Napoli) 9:347–357
Di Rienzo JA, Casanoves F, Gonzales LA, et al (2008) Estadística para las Ciencias Agropecuarias, Séptima ed. Editorial Brujas, Córdoba, Argentina
Fiel CA, Steffan PE, Ferreyra DA (2011) Diagnóstico de las parasitosis más frecuentes de los rumiantes: técnicas de diagnóstico e interpretación de resultados. Abad Benjamin, Tandil, Argentina
Henriksen SA, Korsholm H (1983) A method for culture and recovery of gastrointestinal strongyle larvae. Nord Vet Med 35:429–430
Hsu CK, Levine ND (1977) Degree-day concept in development of infective larvae of Haemonchus contortus and Trichostrongylus colubriformis under constant and cyclic conditions. Am J Vet Res 38:1115–1119
Hubert J, Kerboeuf D (1984) A new method for culture of larvae used in diagnosis of ruminant gastrointestinal strongylosis: Comparison with fecal cultures. Can J Comp Med 48:63–71
Le Jambre LF, Dominik S, Eady SJ et al (2007) Adjusting worm egg counts for faecal moisture in sheep. Vet Parasitol 145:108–115. https://doi.org/10.1016/j.vetpar.2006.11.017
Lysyk TJ, Easton ER, Evenson PD (1985) Seasonal Changes in Nitrogen and Moisture Content of Cattle Manure in Cool-Season Pastures. J Range Manag 38:251–254
Mauleon H, Gruner L (1984) Influence de la déshydratation des féces d’ovins sur l’évolution des stades libres de strongles gastro-intestinaux. Ann Rech Vétérinaires 15:519–528
Nwosu CO, Iwuoha CL, Torru C, Mohammed A (2006) Prevalence Of Caprine Strongyle Infection And The Diagnostic Efficacy Of Some Media For Faecal Culture And Nematode Larval Recovery From Goat Faeces. Anim Res Int 3:419–421. https://doi.org/10.4314/ari.v3i1.40762
O’Connor LJ, Walkden-Brown SW, Kahn LP (2006) Ecology of the free-living stages of major trichostrongylid parasites of sheep. Vet Parasitol 142:1–15. https://doi.org/10.1016/j.vetpar.2006.08.035
Reinecke RK (1973) The larval anthelmintic test in ruminants. In: Technical communication N° 106. Departament of Agricultural Technical Services, Pretoria, South Africa
Roberts FHS, O’Sullivan PJ (1950) Methods for egg counts and larval cultures for strongyles infesting the gastro-intestinal tract of cattle. Aust J Agric Res 1:99–103. https://doi.org/10.1071/AR9500099
Rossanigo CE, Gruner L (1995) Moisture and temperature requirements in faeces for the development of free-living stages of gastrointestinal nematodes of sheep, cattle and deer. J Helminthol 69:357–362
Steffan P, Henriksen SA, Nansen P (1989) A Comparison of Two Methods and Two Additives for Faecal Cultivation of Bovine Trichostrongyle Larvae. Vet Parasitol 31:269–273
Acknowledgements
The revision and edition of English style by the Licenciada en Lengua y Literatura Inglesa Claudia Pérez Pastor is greatly acknowledged.
Funding
The study was partially supported by the Agencia Nacional de Promoción Científica y Tecnológica, Ministerio de Ciencia, Tecnología e Innovación Productiva (grant No PICT 2015-0940), Argentina.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Ethics approval
Uses and practices with experimental animals were approved by the Ethics Committee according to the Animal Welfare Policy (act 087/02) of the Faculty of Veterinary Sciences, UNCPBA, Tandil, Argentina (http://www.vet.unicen.edu.ar).
Consent for publication
Informed consent was obtained from all individual participants included in the study.
Competing interests
The authors declare no competing interests.
Additional information
Section Editor: Georg von Samson-Himmelstjerna
Publisher’s note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
About this article
Cite this article
Muchiut, S., Fernández, S., Domínguez, P. et al. Influence of faecal culture media and incubation time on the yield of infective larvae of Haemonchus contortus (Rudolphi 1803). Parasitol Res 120, 1493–1497 (2021). https://doi.org/10.1007/s00436-021-07078-y
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00436-021-07078-y