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Detection of Leishmania (Viannia) braziliensis in bats from Espírito Santo, Brazil (2018–2019)

  • Protozoology - Original Paper
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Abstract

Studying the occurrence of Leishmania in bats would help toward clarifying the role of these animals in epidemiological cycles. Therefore, the objective of this study was to detect Leishmania spp. in bats from the Brazilian State of Espírito Santo. In total, 105 bats from 11 species which had been collected from 17 municipalities of Espírito Santo were obtained from the IDAF Rabies Diagnostic Laboratory between December 2018 and June 2019. DNA was extracted from the bone marrow, skin, and spleen samples and analysed using conventional PCR, with primers specific for the kinetoplastic DNA minicircle of the parasitic species Leishmania (Viannnia) braziliensis, Leishmania (Leishmania) infantum, and Leishmania (Leishmania) amazonensis. To identify the Leishmania species, the positive PCR products were analysed using PCR-RFLP with the HaeIII enzyme and 8% polyacrylamide gel electrophoresis. Positivity for L. (V.) braziliensis was observed in five (4.76%) bats: namely, one male Eumops glaucinus from Vitória (in a skin sample), one male Artibeus lituratus from Vila Velha and one female Eumops glaucinus from Vitória (both in bone marrow samples), and one male A. lituratus from Vitória and one male Molossus rufus from Guarapari (both in the spleen samples). To our best knowledge, this is the first report of the detection of Leishmania in bats from Espírito Santo as well as of Leishmania DNA in the bone marrow of bats.

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All data generated or analysed during this study are included in this published article.

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Funding

This study was financed in part by the Fundação de Amparo à Pesquisa e Inovação do Estado do Espírito Santo (FAPES) and the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES) - Finance Code 001.

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Authors and Affiliations

  1. Programa de Pós-Graduação em Ciências Veterinárias, Universidade Federal do Espírito Santo, Alto Universitário, s/n, Guararema, Alegre, Espírito Santo, CEP 29.500-000, Brazil

    Mayara Mezabarba Riva, Felipe Martins Pastor, Marcos Santos Zanini & Maria Aparecida da Silva

  2. Departamento de Medicina Veterinária, Universidade Federal do Espírito Santo, Alto Universitário, s/n, Guararema, Alegre, Espírito Santo, CEP 29.500-000, Brazil

    Yuri Vieira Almeida, Lívia Martino Duarte, Lígia Isabelle Silva Souza & Marcos Santos Zanini

  3. Departamento de Biologia, Centro de Ciências Exatas, Naturais e da Saúde, Alto Universitário, s/n, Guararema, Alegre, Espírito Santo, CEP 29.500-000, Brazil

    Maria Aparecida da Silva

Authors
  1. Mayara Mezabarba Riva
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  2. Felipe Martins Pastor
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  3. Yuri Vieira Almeida
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  4. Lívia Martino Duarte
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  5. Lígia Isabelle Silva Souza
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  6. Marcos Santos Zanini
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  7. Maria Aparecida da Silva
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Contributions

All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by Mayara Mezabarba Riva, Felipe Martins Pastor, Yuri Vieira Almeida, Lívia Martino Duarte, Lígia Isabelle Silva Souza, Marcos Santos Zanini, Maria Aparecida da Silva. The first draft of the manuscript was written by Mayara Mezabarba Riva and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Maria Aparecida da Silva.

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Riva, M.M., Pastor, F.M., Almeida, Y.V. et al. Detection of Leishmania (Viannia) braziliensis in bats from Espírito Santo, Brazil (2018–2019). Parasitol Res 120, 3857–3863 (2021). https://doi.org/10.1007/s00436-020-07042-2

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  • DOI: https://doi.org/10.1007/s00436-020-07042-2

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