Skip to main content
Log in

A new myxosporean species, Henneguya lata n. sp. (Myxozoa: Myxobolidae), from the gills of yellowfin seabream Acanthopagrus latus (Perciformes: Sparidae) in the Gulf of Tonkin, Vietnam

  • Fish Parasitology - Original Paper
  • Published:
Parasitology Research Aims and scope Submit manuscript

Abstract

During a survey of myxosporean parasites of marine fish in the coastal region of Vietnam, a species of the genus Henneguya (Myxosporea: Bivalvulida) was found in the gill of yellowfin seabream Acanthopagrus latus (Perciformes: Sparidae). White and oval cysts, measuring 145–220 μm in diameter, were detected in the gill lamellae of 4 of 15 fish examined (26.7%). Mature myxospores were elongate, with smooth valves, two similar polar capsules, and having the following dimensions: spore body length 9.9 ± 0.5 (8.9–12.5) μm, body width 6.7 ± 0.3 (6.1–7.6) μm, thickness 5.1 ± 0.2 (4.8–5.4) μm, caudal appendage length 10.0 ± 1 (8.3–11.6) μm, and total myxospore length 19.3 ± 1.4 (16.5–21.5) μm. The polar capsules were ovoid, measuring 3.2 ± 0.2 (2.8–3.9) μm long and 1.9 ± 0.2 (1.5–2.3) μm wide. Each polar capsule has a polar filament with 4–5 coils. Histological analysis revealed plasmodia in the connective tissues of the gill lamellae, but inflammation and deformation of the gills were not observed. In the phylogenetic tree reconstructed from the small subunit ribosomal DNA (SSU rDNA), sequences of the Henneguya specimens found in this study form a distinct branch. Morphological characteristics and molecular data identified a new species, namely Henneguya lata n. sp.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  • Arthur JR, Te BQ (2006) Checklist of the parasites of fishes of Viet Nam. FAO Fisheries Technical paper 369/2. Food and Agriculture Organization of the United Nations, Rome

  • Barta JR, Martin DS, Liberator PA, Dashkevicz M, Anderson JW, Feighner SD, Elbrecht A, Perkins-Barrow A, Jenkins MC, Danforth HD, Ruff MD, Profous-Juchelka H (1997) Phylogenetic relationships among eight Eimeria species infecting domestic fowl inferred using complete small subunit ribosomal DNA sequences. J Parasitol 83:262–271

    Article  CAS  Google Scholar 

  • Borkhanuddin MH, Cech G, Molnár K, Shaharom-Harrison F, Khoa TND, Samshuri MA, Mazelan S, Atkinson SD, Székely C (2020) Henneguya (Cnidaria: Myxosporea: Myxobolidae) infections of cultured barramundi, Lates calcarifer (Perciformes: Latidae) in an estuarine wetlands system of Malaysia: description of Henneguya setiuensis n. sp., Henneguya voronini n. sp. and Henneguya calcarifer n. sp. Parasitol Res 119(1):85–96. https://doi.org/10.1007/s00436-019-06541-1

    Article  PubMed  Google Scholar 

  • Carriero MM, Adriano EA, Silva MR, Ceccarelli PS, Maia AA (2013) Molecular phylogeny of the Myxobolus and Henneguya genera with several new South American species. PLoS One 8:e73713. https://doi.org/10.1371/journal.pone.0073713

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Chaudhary A, Gupta A, Goswami U, Cech G, Molnár K, Singh HS, Székely C (2019) Molecular genetic studies on Myxobolus cylindricus and Henneguya mystasi (Myxosporea: Myxobolidae) infecting two Indian fish species, Channa gachua and Mystus vittatus, respectively. Acta Parasit. 64:129–137. https://doi.org/10.2478/s11686-018-00014-8

    Article  CAS  Google Scholar 

  • Chen W, Yang C, Zhao Y (2020) Characterization of Myxidium spinibarba sp. nov. (Cnidaria, Myxosporea, Myxidiidae) from Spinibarbus sinensis (Bleeker, 1871) in Chongqing China. Parasitol Res 119:1485–1491. https://doi.org/10.1007/s00436-020-06644-0

    Article  PubMed  Google Scholar 

  • Eiras JC (2002) Synopsis of the species of the genus Henneguya Thélohan, 1892 (Myxozoa: Myxosporea: Myxobolidae). Syst Parasitol 52:43–54. https://doi.org/10.1023/A:1015016312195

    Article  CAS  PubMed  Google Scholar 

  • Eiras JC, Adriano EA (2012) A checklist of new species of Henneguya Thélohan, 1892 (Myxozoa: Myxosporea, Myxobolidae) described between 2002 and 2012. Syst Parasitol 83:95–104. https://doi.org/10.1007/s11230-012-9374-7

    Article  CAS  PubMed  Google Scholar 

  • Fiala I (2006) The phylogeny of Myxosporea (Myxozoa) based on small subunit ribosomal RNA gene analysis. Int J Parasitol 36:1521–1534. https://doi.org/10.1016/j.ijpara.2006.06.016

    Article  CAS  PubMed  Google Scholar 

  • Fiala I, Bartošová-Sojková P, Okamura B, Hartikainen H (2015) Adaptive radiation and evolution within the myxozoa. In: Okamura B, Gruhl A, Bartholomew J (eds) Myxozoan evolution, ecology and development. Springer, New York, pp 69–84. https://doi.org/10.1007/978-3-319-14753-6_4

    Chapter  Google Scholar 

  • Ganapati PN (1941) On a new Myxosporidian henneguya otolithi n. sp. a tissue parasite from the bulbus arteriosus of two species of fish of the genus Otolithus. Proceedings of the Indian Academy of Sciences - Section B 13:135–150. https://doi.org/10.1007/BF03049148

    Article  Google Scholar 

  • Haldar DP, Mukherjee M (1985) Studies on bivalvulida (Myxozoa: Myxosporea): observations on two new species of Henneguya from food fishes of West Bengal, India. Arch Protistenkd 130:419–425. https://doi.org/10.1016/S0003-9365(85)80053-1

    Article  Google Scholar 

  • Hall DL, Iversen ES (1967) Henneguya lagodon, a new species of myxosporidian parasitizing the pinfish, Lagodon rhomboides. Bull Mar Sci 17:274–279

    Google Scholar 

  • Kawai T, Sekizuka T, Yahata Y, Kuroda M, Kumeda Y, Iijima Y, Kamata Y, Sugita-Konishi Y, Ohnishi T (2012) Identification of Kudoa septempunctata as the causative agent of novel food poisoning outbreaks in Japan by consumption of Paralichthys olivaceus in raw fish. Clin Infect Dis 54:1046–1052. https://doi.org/10.1093/cid/cir1040

    Article  PubMed  Google Scholar 

  • Kent ML, Andree KB, Bartholomew JL, El-Matbouli M, Desser SS, Devlin RH, Feist SW, Hedrick RP, Hoffmann RW, Khattra J, Hallett SL, Lester RJ, Longshaw M, Palenzeula O, Siddall ME, Xiao C (2001) Recent advances in our knowledge of the Myxozoa. J Eukaryot Microbiol 48:395–413. https://doi.org/10.1111/j.1550-7408.2001.tb00173.x

    Article  CAS  PubMed  Google Scholar 

  • Kpatcha TK, Faye N, Diebakate C, Fall M, Toguebaye BS (1997) Nouvelles espèces d’Henneguya Thélohan, 1895 (Myxozoa, Myxosporea) parasites des poissons marins du Sénégal: Étude en microscopie photonique et électronique. Ann Sci nat, Zoologie 18:81-91

  • Ky H (1971) Some myxosporidians of freshwater fishes of North Viet Nam. Acta Protozool 8:283–298

    Google Scholar 

  • Ky H, Te BQ (2007) Parasites on freshwater fish in Viet Nam. Science and Technology, Ha Noi

    Google Scholar 

  • Li YC, Sato H, Kamata Y, Ohnishi T, Sugita-Konishi Y (2012) Three novel myxobolid species of genera Henneguya and Myxobolus (Myxosporea: Bivalvulida) from marine fish in Japan. Parasitol Res 111:819–826. https://doi.org/10.1007/s00436-012-2904-z

    Article  PubMed  Google Scholar 

  • Lom J, Arthur JR (1989) A guideline for the preparation of species descriptions in Myxosporea. J Fish Dis 12:151–156. https://doi.org/10.1111/j.1365-2761.1989.tb00287.x

    Article  Google Scholar 

  • Lom J, Dyková I (2006) Myxozoan genera: definition and notes on taxonomy, life-cycle terminology and pathogenic species. Folia Parasitol 53:1-36. https://doi.org/10.14411/fp.2006.001

  • Luna LG (1968) Manual of histologic staining methods of the armed forces institute of pathology, 3rd edn. McGraw-Hill, New York

    Google Scholar 

  • Ministry of Health (2016) A guide to technical procedure for histopathology-cytology. Medical Publishing House, Hanoi

    Google Scholar 

  • Naldoni J, Arana S, Maia AA, Ceccarelli PS, Tavares LE, Borges FA, Pozo CF, Adriano EA (2009) Henneguya pseudoplatystoma n. sp. causing reduction in epithelial area of gills in the farmed pintado, a South American catfish: histopathology and ultrastructure. Vet Parasitol 166:52–59. https://doi.org/10.1016/j.vetpar.2009.07.034

    Article  CAS  PubMed  Google Scholar 

  • Özer A, Özkan H, Gürkanlı CT, Yurakhno V, Çiftçi Y (2016) Morphology, histology and phylogeny of Henneguya sinova sp. nov. (Myxobolidae: Myxozoa) infecting gills of Parablennius tentacularis in the Black Sea, Turkey. Dis Aquat Organ 118:207–215. https://doi.org/10.3354/dao02968

    Article  CAS  PubMed  Google Scholar 

  • Phu VV, Dao LT (2011) Reproductive biology characteristics of Acanthopagrus latus (Houttuyn, 1782) in coastal zone of Thua thien Hue province. Hue University Journal of Science 64:77–84

    Google Scholar 

  • Pote LMW, Khoo L, Griffin M (2012) Henneguya ictaluri. In: Woo PTK, Buchmann K (eds) Fish parasites: pathobiology and protection. CAB International, Oxfordshire, pp 177–192

    Chapter  Google Scholar 

  • Reed CC, Basson L, Van As LL, Dyková I (2007) Four new myxozoans (Myxosporea: Bivalvulida) from intertidal fishes along the south coast of Africa. Folia Parasitol 54:283-292. https://doi.org/10.14411/fp.2007.037

  • Shin SP, Nguyen VG, Jeong JM, Jun JW, Kim JH, Han JE, Baeck GW, Park SC (2014) The phylogenetic study on Thelohanellus species (Myxosporea) in relation to host specificity and infection site tropism. Mol Phylogenet Evol 72:31–34. https://doi.org/10.1016/j.ympev.2014.01.002

    Article  PubMed  Google Scholar 

  • Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Mol Biol Evol 30:2725–2729. https://doi.org/10.1093/molbev/mst197

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Wagner EJ (2016) A guide to the identification of tailed Myxobolidae of the World: Dicauda, Hennegoides, Henneguya, Laterocapsulata, Neohenneguya, Phlogospora, Tetrauronema, Trigonosporus, and Unicauda. Fish Creek Records, Logan, Utah

  • Work TM, Takata G, Whipps CM, Kent ML (2008) A new species of Henneguya (Myxozoa) in the big-eyed scad (Selar crumenophthalmus) from Hawaii. J Parasitol 94:524–529. https://doi.org/10.1645/GE-1385.1

    Article  PubMed  Google Scholar 

  • Yokoyama H, Kawakami H, Yasuda H, Tanaka S (2003) Henneguya lateolabracis sp. n. (Myxozoa: Myxosporea), the causative agent of cardiac henneguyosis in Chinese sea bass Lateolabrax sp. Fish Sci 69:1116–1120. https://doi.org/10.1111/j.0919-9268.2003.00736.x

    Article  CAS  Google Scholar 

  • Yokoyama H, Itoh N, Tanaka S (2005) Henneguya pagri n. sp. (Myxozoa: Myxosporea) causing cardiac henneguyosis in red sea bream, Pagrus major (Temminck & Schlegel). J Fish Dis 28:479–487. https://doi.org/10.1111/j.1365-2761.2005.00655.x

    Article  CAS  PubMed  Google Scholar 

  • Yurakhno VM, Ha VT (2019) First data on bivalvulida myxosporeans of Nha Trang bay mullets (Vietnam). Mar Biol J 4:82-88. https://doi.org/10.21072/mbj.2019.04.4.07

  • Zatti SA, Naldoni J, Silva MR, Maia AA, Adriano EA (2015) Morphology, ultrastructure and phylogeny of Myxobolus curimatae n. sp. (Myxozoa: Myxosporea) a parasite of Prochilodus costatus (Teleostei: Prochilodontidae) from the São Francisco River, Brazil. Parasitol Int 64:362–368. https://doi.org/10.1016/j.parint.2015.05.011

    Article  PubMed  Google Scholar 

Download references

Acknowledgments

We sincerely thank Professor Ken MacKenzie from the School of Biological Sciences (Zoology), University of Aberdeen (UK) for his valuable comments and editing the manuscript.

Funding

This study was supported by the Vietnam National Program No. 47 under Grant code VAST.DA47.12/16-19 and the project of the Institute of Ecology and Biological Resources under code IEBR ThST.1-20.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Nguyen Ngoc Chinh.

Additional information

Section Editor: Christopher Whipps

Publisher’s note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Chinh, N.N., Ngo, H.D., Van Tuc, V. et al. A new myxosporean species, Henneguya lata n. sp. (Myxozoa: Myxobolidae), from the gills of yellowfin seabream Acanthopagrus latus (Perciformes: Sparidae) in the Gulf of Tonkin, Vietnam. Parasitol Res 120, 877–885 (2021). https://doi.org/10.1007/s00436-020-07031-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00436-020-07031-5

Keywords

Navigation