Abstract
This study evaluated the technology of detection of Giardia spp. cysts and Cryptosporidium spp. oocysts in environmental matrices obtained after water treatment on a bench scale. Calcium carbonate flocculation with immunomagnetic separation was the selected method to quantify the protozoa, and the importance of the number of acid dissociations in the immunomagnetic separation was assessed. When adding the third acid dissociation, an increase of 71% ± 6 in floated residue and 31.9% ± 28.7 in filter backwash water in cyst recovery was observed, while in oocyst recovery, a non-significant increase was detected. In the filtered water, this increased dissociation was important in the protozoa recovery with increases greater than 33%. The results showed that there is a strong interaction of these target organisms with the magnetic microspheres, since protozoa were still recovered in the third acid dissociation and some of them were still adhered to the magnetic microspheres.
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Funding
The authors are grateful to the São Paulo Research Foundation (FAPESP) (Process 12/50522-0), the Global Challenges Research Fund (GCRF) UK Research and Innovation (SAFEWATER; EPSRC Grant Reference EP/P032427/1) for the research support and the National Council for Scientific and Technological Development (CNPq-Brazil) for the Master’s scholarship awarded to Fernando César Andreoli.
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Andreoli, F.C., Sabogal-Paz, L.P. Detection of Giardia and Cryptosporidium in environmental matrices with immunomagnetic separation: two or three acid dissociations. Parasitol Res 120, 629–635 (2021). https://doi.org/10.1007/s00436-020-06999-4
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DOI: https://doi.org/10.1007/s00436-020-06999-4