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Renicolidae infection in Manx shearwater (Puffinus puffinus): is parasitism implicated on renal lesions?

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Abstract

Renicolid digeneans are frequently observed in the renal tubules and ureters of seabirds, such Puffinus puffinus, a migratory species distributed along the Brazilian coast. However, few studies have focused on the relationship between renicolid infection and health status in P. puffinus. Thus, the aim of this study was to describe (i) renal and systemic alterations, (ii) the renicolids and (iii) the biological aspects associated with the presence of renicolids in P. puffinus. Gross and histological assays were performed in 93 P. puffinus stranded on the Paraná coast, southern Brazil, and renicolids were submitted to morphological and molecular assays. A high prevalence of renicolids in P. puffinus (71/93) was observed. In the kidney, the main microscopic findings were lymphocytic interstitial infiltrate, ductal ectasia and tubular necrosis. The renal lesions were significantly associated with the parasite infection. The morphological (n = 84) and molecular analyses (n = 2) confirmed the species as Renicola sloanei (100% and 95.9% of nucleotide identity with R. sloanei strains from P. puffinus and from Spheniscus demersus, respectively). In both parasitized and non-parasitized animals, cardiac and skeletal muscle degeneration and necrosis were the most frequent systemic changes. Therefore, the results suggest renicolids being a possible cause for the demonstrated renal alterations. A contribution of this parasite to a decreased health status of Puffinus puffinus along their migratory route is possible.

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The study was performed in collaboration with the Beach Monitoring Project of the Santos Basin in Paraná, a requirement established by the federal environmental licencing division of the Brazilian Environmental Agency (IBAMA) to explore the presalt.

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Acknowledgments

The authors would like to thank the collaborators of the Beach Monitoring Project of the Santos Basin in Paraná, a requirement established by the federal environmental licencing division of the Brazilian Environmental Agency (IBAMA) and Ângela Teresa Silva e Souza for the contribution with the histology of the parasites. In addition, this study was the subject of the MSc thesis by Matos, A. at Universidade Estadual de Londrina.

Funding

Matos A. and Bracarense A. P. were financed by grants from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) from the Brazilian government.

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Authors and Affiliations

  1. Laboratory of Animal Pathology, Universidade Estadual de Londrina, Londrina, Paraná, Brazil

    Andressa Maria Rorato Nascimento de Matos, Thiago Gallo Bizari, Ricardo Luis Nascimento de Matos & Ana Paula Frederico Rodrigues Loureiro Bracarense

  2. Laboratory of Ecology and Conservation, Center for Marine Studies, Universidade Federal do Paraná, Pontal do Sul, Paraná, Brazil

    Mário Roberto Castro Meira-Filho & Camila Domit

  3. Laboratory of Animal Virology, Universidade Estadual de Londrina, Londrina, Paraná, Brazil

    Elis Lorenzetti & Fernanda Louise Pereira Lavorente

  4. Laboratory of Animal Parasitology, Universidade Estadual de Londrina, Londrina, Paraná, Brazil

    Eloiza Teles Caldart

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  1. Andressa Maria Rorato Nascimento de Matos
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Contributions

Andressa M. Rorato N. de Matos: delineating of the study, molecular and morphological analysis, histopathology, and writing

Mário R. C. Meira Filho: parasitological analysis and writing

Elis Lorenzetti and Fernanda L. L. Pereira: molecular analysis and writing

Eloiza T. Caldart: statistical evaluations and writing

Thiago Bizari and Ricardo L. N. de Matos: histological analysis

Camila Domit: critical revision

Ana Paula F. R. L. Bracarense: delineating of the study, writing and critical revision

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Correspondence to Ana Paula Frederico Rodrigues Loureiro Bracarense.

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de Matos, A.M.R.N., Meira-Filho, M.R.C., Lorenzetti, E. et al. Renicolidae infection in Manx shearwater (Puffinus puffinus): is parasitism implicated on renal lesions?. Parasitol Res 120, 1311–1320 (2021). https://doi.org/10.1007/s00436-020-06959-y

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