Skip to main content
SpringerLink
Log in

A new species, Henneguya lacustris n. sp. (Cnidaria: Myxosporea), infecting the gills of Astyanax lacustris from Brazil

  • Fish Parasitology - Short Communication
  • Published:
Parasitology Research Aims and scope Submit manuscript

Abstract

The present study describes Henneguya lacustris n. sp. parasitizing Astyanax lacustris (Lütken 1875), from the Tietê River, State of São Paulo, Brazil, through morphological and molecular analysis. Myxospores of the parasites were found in the gills of A. lacustris with 13% prevalence of infection. The myxospores were oval and presented as measures (mean ± standard deviation): total length 18.3 ± 2.2 μm, body length 10.4 ± 1.6 μm, body width 4.9 ± 0.9 μm, tail length 7.2 ± 2.5 μm. The polar capsule was 4.8 ± 0.3 μm long and 1.5 ± 0.2 μm wide. The polar tubules, present inside the polar capsules, had 6 to 7 turns. The ultrastructural analysis allowed the observation of the asynchronous development of the myxospores. Phylogenetic analysis showed H. lacustris n. sp. as a sister species of H. chydadea Barassa and Cordeiro 2003 in a subclade formed by species that parasitize the gills of Characiform fish from Brazil. Using molecular and morphological characterization, this parasite was identified as a new species of the genus Henneguya Thélohan 1892.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

References

  • Barassa B, Cordeiro NS, Arana S (2003) A new species of Henneguya, a gill parasite of Astyanax altiparanae (Pisces : Characidae) from Brazil, with comments on histopathology and seasonality. Mem Inst Oswaldo Cruz 98:761–765

    Article  Google Scholar 

  • Bartošová-Sojková P, Hrabcová M, Pecková H, Patra S, Kodádková A, Jurajda P, Holzer AS (2014) Hidden diversity and evolutionary trends in malacosporean parasites (Cnidaria: Myxozoa) identified using molecular phylogenetics. Int J Parasitol 44:565–577

    Article  Google Scholar 

  • Bush AO, Lafferty KD, Lotz JM, Shostak AW (1997) Parasitology meets ecology on its own terms: Margolis et al. revisited. J Parasitol 83:575–583

    Article  CAS  Google Scholar 

  • Casal G, Matos E, Azevedo C (2003) Light and electron microscopic study of the myxosporean, Henneguya friderici n. sp from the Amazonian teleostean fish, Leporinus friderici. Parasitol 126:313–319

    Article  CAS  Google Scholar 

  • Casal G, São Clemente SC, Lopes L, Rocha S, Felizardo N, Oliveira E, Al Quraishy S, Azevedo C (2017) Ultrastructural morphology and phylogeny of Henneguya gilbert n. sp. (Myxozoa) infecting the teleostean Cyphocharax gilbert (Characiformes: Curimatidae) from Brazil. Parasitol Res 116:2747–2756

    Article  Google Scholar 

  • Cech G, Molnar K, Szekely C (2012) Molecular genetic studies on morphologically indistinguishable Myxobolus spp. infecting cyprinid fishes, with the description of three new species, M. alvarezae sp nov., M. sitjae sp nov and M. eirasianus sp nov. Acta Parasitol 57:354–366

    Article  Google Scholar 

  • Fiala I (2006) The phylogeny of Myxosporea (Myxozoa) based on small subunit ribosomal RNA gene analysis. Int J Parasitol 36(14):1521–1534

  • Lima FCT de, Malabarba LR, Buckup PA, Silva JF, Vari RP, Benine R, Oyakawa OT, Pavanelli CS, Menezes NA, Lucena CAS (2003) Genera incertae sedis in Characidae. Check List Freshw fishes South Cent Am

  • Eiras JC, Adriano EA (2012) A checklist of new species of Henneguya Thelohan, 1892 (Myxozoa: Myxosporea, Myxobolidae) described between 2002 and 2012. Syst Parasitol 83:95–104

    Article  CAS  Google Scholar 

  • Gioia I, da Silva Cordeiro N, de Toledo Artigas P (1986) Henneguya intracornea n. sp.(Myxozoa: Myxosporea) parasita do olho do lambari, Astyanax scabripinnis (Jenyns, 1842) (Osteichthyes, Characidae). Mem Inst Oswaldo Cruz 81:401–407

    Article  Google Scholar 

  • Hallett SL, Diamant A (2001) Ultrastructure and small-subunit ribosomal DNA sequence of Henneguya lesteri n. sp (Myxosporea), a parasite of sand whiting Sillago analis (Sillaginidae) from the coast of Queensland, Australia. Dis Aquat Org 46:197–212

    Article  CAS  Google Scholar 

  • Hillis DM, Dixon MT (1991) Ribosomal DNA: molecular evolution and phylogenetic inference. Q Rev Biol 66:411–453

    Article  CAS  Google Scholar 

  • Kearse M, Moir R, Wilson A, Stones-Havas S, Cheung M, Sturrock S, Buxton S, Cooper A, Markowitz S, Duran C (2012) Geneious basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics 28:1647–1649

    Article  Google Scholar 

  • Kumar S, Stecher G, Tamura K (2016) MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 33:1870–1874

    Article  CAS  Google Scholar 

  • Larkin MA, Blackshields G, Brown NP, Chenna R, Mcgettigan PA, McWilliam H, Valentin F, Wallace IM, Wilm A, Lopez R, Thompson JD, Gibson TJ, Higgins DG (2007) Clustal W and Clustal X version 2.0. Bioinformatics 23:2947–2948

    CAS  Google Scholar 

  • Lom J, Arthur JR (1989) A guideline for the preparation of species descriptions in Myxosporea. J Fish Dis 12:151–156

    Article  Google Scholar 

  • Lom J, Dykova I (2006) Myxozoan genera: definition and notes on taxonomy, life-cycle terminology and pathogenic species. Folia Parasitol 53:1–36

    Article  Google Scholar 

  • Martins ML, De Souza VN, Moraes JR, De Moraes FR (1999) Gill infection of Leporinus macrocephalus Garavello & Britski, 1988 (Osteichthyes: Anostomidae) by Henneguya leporinicola n. sp. (Myxozoa: Myxobolidae). Description, histopathology and treatment. Rev Bras Biol 59:527–534

    Article  CAS  Google Scholar 

  • Negrelli DC, Vieira DHMD, Tagliavini VP, Abdallah VD, de Azevedo RK (2019) Molecular and morphological analysis of Henneguya jundiai n. sp.(Cnidaria: Myxosporea), a new parasite of the gills of Rhamdia quelen in Brazil. Acta Trop 197:105053

    Article  CAS  Google Scholar 

  • Nelson JS, Grande TC, Wilson MVH (2016) Fishes of the world. Wiley

  • Posada D (2008) JModelTest: Phylogenetic model averaging. Mol Biol Evol 25:1253–1256

    Article  CAS  Google Scholar 

  • Pote LM, Hanson LA, Shivaji R (2000) Small subunit ribosomal RNA sequences link the cause of proliferative gill disease in channel catfish to Henneguya n. sp (Myxozoa : Myxosporea). J Aquat Anim Health 12:230–240

    Article  Google Scholar 

  • Rambaut A (2014) FigTree 1.4. 2 software. Inst Evol Biol Univ Edinburgh

  • Ronquist F, Huelsenbeck JP (2003) MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19:1572–1574

    Article  CAS  Google Scholar 

  • Schlegel M, Lom J, Stechmann A, Bernhard D, Leipe D, Dyková I, Sogin ML (1996) Phylogenetic analysis of complete small subunit ribosomal RNA coding region of Myxidium lieberkuehni: evidence that Myxozoa are Metazoa and related to the Bilateria. Arch Protistenkd 147:1–9

    Article  Google Scholar 

  • Velasco M, Videira M, Do Nascimento LDCS, Matos P, Gonçalves EC, Matos E (2016) Henneguya paraensis n. sp. (Myxozoa; Myxosporea), a new gill parasite of the Amazonian fish Cichla temensis (Teleostei: Cichlidae): morphological and molecular aspects. Parasitol Res 115:1779–1787

    Article  Google Scholar 

  • Vieira DHMD, Tagliavini VP, Abdallah VD, de Azevedo RK (2018) Myxobolus imparfinis n. sp. (Myxozoa: Myxosporea), a new gill parasite of Imparfinis mirini Haseman (Siluriformes: Heptapteridae) in Brazil. Syst Parasitol 95:309–318

    Article  Google Scholar 

  • Vita P, Corral L, Matos E, Azevedo C (2003) Ultrastructural aspects of the myxosporean Henneguya astyanax n. sp (Myxozoa : Myxobolidae), a parasite of the Amazonian teleost Astyanax keithi (Characidae). Dis Aquat Org 53:55–60

    Article  Google Scholar 

  • Whipps CM, Kent ML (2006) Phylogeography of the cosmopolitan marine parasite Kudoa thyrsites (Myxozoa: Myxosporea). J Eukaryot Microbiol 53:364–373

    Article  CAS  Google Scholar 

  • Whipps CM, Adlard RD, Bryant MS, Lester RJG, Findlay V, Kent ML (2003) First report of three Kudoa species from eastern Australia: Kudoa thyrsites from Mahi mahi (Coryphaena hippurus), Kudoa amamiensis and Kudoa minithyrsites n. sp from sweeper (Pempheris ypsilychnus). J Eukaryot Microbiol 50:215–219

    Article  Google Scholar 

  • Yokoyama H, Itoh N, Tanaka S (2005) Henneguya pagri n. sp. (Myxozoa: Myxosporea) causing cardiac henneguyosis in red sea bream, Pagrus major (Temminck & Schlegel). J Fish Dis 28:479–487

    Article  CAS  Google Scholar 

Download references

Funding

The authors thank the Fundação de Apoio à Pesquisa do Estado de São Paulo - FAPESP (Process num. 2014/12862-0, 2015/24901-2, 2019/07708-5 and 2019/19060-0) for the scientific and financial support. This work was partially supported by national funds through FCT - Foundation for Science and Technology within the scope of UIDB/04423/2020 and UIDP/04423/2020.

Author information

Authors and Affiliations

  1. Departament of Parasitology, Universidade Estadual Paulista “Júlio de Mesquita Filho” – Unesp, R. Prof. Dr. Antônio Celso Wagner Zanin, 250 - Distrito de Rubião Junior, Botucatu, São Paulo, CEP: 18618-689, Brazil

    Diego Henrique Mirandola Dias Vieira & Vinicius Panciera Tagliavini

  2. Faculdade de Ciências e CIIMAR – Porto, Universidade do Porto, Porto, Portugal

    Luís Filipe Rangel & Maria João Santos

  3. Programa de Pós-graduação em Análise de Sistemas Ambientais, Centro Universitário CESMAC, Maceió, Alagoas, Brazil

    Vanessa Doro Abdallah & Rodney Kozlowiski de Azevedo

Authors
  1. Diego Henrique Mirandola Dias Vieira
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Luís Filipe Rangel
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Vinicius Panciera Tagliavini
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Vanessa Doro Abdallah
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Maria João Santos
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Rodney Kozlowiski de Azevedo
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Diego Henrique Mirandola Dias Vieira.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

Additional information

Section Editor: Astrid Holzer

Publisher’s note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Vieira, D.H.M.D., Rangel, L.F., Tagliavini, V.P. et al. A new species, Henneguya lacustris n. sp. (Cnidaria: Myxosporea), infecting the gills of Astyanax lacustris from Brazil. Parasitol Res 119, 4259–4265 (2020). https://doi.org/10.1007/s00436-020-06871-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00436-020-06871-5

Keywords

Search

Navigation