Abstract
From a longitudinal survey conducted on 30 Danish mink farms in 2016, 11.0% of faecal samples (456/4140) were positive for Cystoisospora laidlawi oocysts by microscopy, with 60% (189/315) of mink being positive at least once during the study period. Morphological analysis of sporulated oocysts identified Cystoisospora oocysts measuring 34.3 × 29.5 μm with an oocyst length/width (L/W) ratio of 1.2. The morphological features of the oocysts were identical to Isospora laidlawi previously morphological identified in farmed mink from Denmark and elsewhere. Phylogenetic analysis of 18S rDNA sequences (1221 bp) from three positive mink indicated that Cystoisospora from mink shared the highest genetic similarity to C. canis from a Canadian dog (99.6%). The phylogenetic analysis placed Cystoisospora from mink in a clade with other Cystoisospora isolates.
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Data availability
The datasets used and analysed during the current study are available from the corresponding author on reasonable request.
References
Anon (2017a) Mink. In: Landbr. og Fødevare, viden om landbrugsproduktion [in Danish]
Anon (2017b) Avlstæver og avlsresultater [in Danish]. http://www.kopenhagenfur.com/da/minkavl/historisk-data/avlstaever-og-avlsresultater. Accessed 19 Sep 2017
Barta JR, Schrenzel MD, Carreno R, Rideout BA (2005) The genus Atoxoplasma (Garnham 1950) as a junior objective synonym of the genus Isospora (Schneider 1881) species infecting birds and resurrection of Cystoisospora (Frenkel 1977) as the correct genus for Isospora species infecting mammals. J Parasitol 91:726–727. https://doi.org/10.1645/GE-3341.1
Bell WB, Threlkeld WL (1948) Isospora laidlawi in mink. Cornell Vet 38:315–316
Chriél M, Hansen MS, Holm E, et al (2013) Rystemink [in Danish]. Fagl årsberetning by Kopenhagen Fur 117–120
Duszynski DW, Couch L, Upton SJ (2000) Coccidia of the world. http://www.k-state.edu/parasitology/worldcoccidia/CARNIV2? Accessed 19 Sep 2017
Fayer R, Dubey JP (1987) Comparative epidemiology of coccidia: clues to the etiology of equine protozoal myeloencephalitis. Int J Parasitol 17:615–620. https://doi.org/10.1016/0020-7519(87)90138-X
Foreyt WJ, Todd AC (1976) Prevalence of Coccidia in domestic mink in Wisconsin. J Parasitol 62:496
Frenkel JK (1977) Besnoitia wallacei of cats and rodents: with a reclassification of other cyst-forming Isosporoid coccidia. J Parasitol 63:611–628. https://doi.org/10.2307/3279560
Hammer AS, Andersen TH, Dietz HH (2004) Forekomsten af coccidier i danske farm mink indsendt til diagnostiske undersøgelser [in Danish]. Kopenhagen Fur, Fagl. årsberetning 223–228
Hindsbo O, Andreassen J, Nielsen F, Lodal J (1995) Occurence of the coccidia Isospora laidlawi and Eimeria vison in Danish farm mink, 1987-1993; age related resistance to the infection. Scientifur 19:231–237
Hoare CA (1927) On the Coccidia of the ferret. Ann Trop Med Parasitol 21:313–320. https://doi.org/10.1080/00034983.1927.11684540
Levine ND (1948) Eimeria and Isospora of the mink (Mustela vison). J Parasitol 34:486–492
McTaggart HS (1960) Coccidia from mink in Britain. J Parasitol 46:201–205. https://doi.org/10.2307/3275170
Nukerbaeva KK, Svanbaev SK (1973) Coccidia of fur animals in Kazakistan [in Russian]. Vestn Sel’skokhoziaistvennoi Nauk Kazakhstana 12:50–54
Petersen HH, Yang R, Chriél M, Hansen MS, Ryan UM (2018) Morphological and molecular characterisation of Eimeria vison-like oocysts (Apicomplexa:Eimeriidae) in farmed mink (Neovison vison) in Denmark. Parasitol Res 117:2933–2939. https://doi.org/10.1007/s00436-018-5989-1
Roepstorff A, Nansen P (1998) Epidemiology, diagnosis and control of helminth parasites of swine. FAO animal health manual, Rome
Svanbaev CK (1956) Coccidia in the field in various mammals [in Russian]. Izv Akad Nauk Kazakhstan SSR 5:180–191
Yang R, Murphy C, Song Y, Ng-Hublin J, Estcourt A, Hijjawi N, Chalmers R, Hadfield S, Bath A, Gordon C, Ryan U (2013) Specific and quantitative detection and identification of Cryptosporidium hominis and C. parvum in clinical and environmental samples. Exp Parasitol 135:142–147. https://doi.org/10.1016/j.exppara.2013.06.014
Yang R, Brice B, Ryan U (2014) A new Caryospora coccidian species (Apicomplexa: Eimeriidae) from the laughing kookaburra (Dacelo novaeguineae). Exp Parasitol 145:68–73. https://doi.org/10.1016/j.exppara.2014.07.008
Yang R, Brice B, Ryan U (2016) Morphological and molecular characterization of Isospora neochmiae n. sp. in a captive-bred red-browed finch (Neochmia temporalis) (Latham, 1802). Exp Parasitol 166:181–188. https://doi.org/10.1016/j.exppara.2016.04.011
Acknowledgements
We thank the participating mink farmers for their support and providing faecal samples throughout the study. The authors also thank the laboratory technicians in the parasitology laboratory at the Centre for Diagnostics, Technical University of Denmark, for skilled technical assistance with analysing the faecal samples.
Funding
This project was funded by the Pelsdyrafgiftsfonden 2016 (www.pelsdyrafgiftsfonden.dk/media/167031/vedt_gter_20-04-12.pdf). Pelsdyrafgiftsfonden is a levy fund, according to law order no. 445, 23 April 2010. The objective of the fund is to support research, with the aim to enable a more sustainable fur production. The fund did not interfere in the study design, data collection, data analysis, or the interpretation of the results.
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Petersen, H.H., Yang, R., Chriel, M. et al. Morphological and molecular characterization of Cystoisospora laidlawi oocysts (Apicomplexa: Eimeriidae) in farmed American mink (Neovison vison) in Denmark. Parasitol Res 119, 3549–3553 (2020). https://doi.org/10.1007/s00436-020-06846-6
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DOI: https://doi.org/10.1007/s00436-020-06846-6