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Angiostrongylus cantonensis infection induces MMP-9 and causes tight junction protein disruption associated with Purkinje cell degeneration

  • Helminthology - Original Paper
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Abstract

Angiostrongylus cantonensis causes a human central nervous system (CNS) infection characterized by eosinophilic meningitis or meningoencephalitis. Individuals infected with A. cantonensis exhibit unbalanced walking. The mechanism of extensive neurological impairments of hosts caused by A. cantonensis larvae remains unclear. Tight junction proteins (e.g., claudin-5 and zonula occludens-1) are the most important regulators of paracellular permeability and cellular adhesion. In a previous study, we found that increased matrix metalloproteinase-9 (MMP-9) activity may be associated with blood–CNS barrier disruption and/or the degeneration of Purkinje cells in eosinophilic meningitis caused by A. cantonensis. In the present study, the co-localization of MMP-9 and tight junction proteins on the degeneration of Purkinje cells was measured via confocal laser scanning immunofluorescence microscopy. The statistical evidence indicated that MMP-9 correlated between tight junction protein disruption and Purkinje cell degeneration at 20 days post-infection with A. cantonensis. In conclusion, Purkinje cell degeneration is highly correlated with tight junction protein disruption via the MMP-9 activation pathway.

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Authors and Affiliations

  1. Department of Parasitology, School of Medicine, Chung Shan Medical University, 110, Section 1, Chien-Kuo North Road, Taichung, 402, Taiwan

    Shih-Chan Lai, Ling-Yuh Shyu & Ke-Min Chen

  2. Cardiovascular and Mitochondrial Related Disease Research Center, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Hualien, 970, Taiwan

    Cheng-You Lu

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  1. Shih-Chan Lai
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  2. Cheng-You Lu
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Correspondence to Ke-Min Chen.

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Lai, SC., Lu, CY., Shyu, LY. et al. Angiostrongylus cantonensis infection induces MMP-9 and causes tight junction protein disruption associated with Purkinje cell degeneration. Parasitol Res 119, 3433–3441 (2020). https://doi.org/10.1007/s00436-020-06840-y

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  • DOI: https://doi.org/10.1007/s00436-020-06840-y

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