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Monogenean parasites from the stomach of Oreochromis mossambicus from South Africa: two new species of Enterogyrus (Dactylogyridae: Ancyrocephalinae)

Abstract

The study provides descriptions of two new species of Enterogyrus coexisting in the stomach of the Mozambique tilapia, Oreochromis mossambicus (Peters). Enterogyrus multispiralis n. sp. and Enterogyrus mashegoi n. sp. were collected from hosts sampled at Nwanedi-Luphephe Dam, Limpopo River System, South Africa. The two new species can be differentiated from other members of the genus based on the spirality characteristics of the cirrus. Enterogyrus multispiralis n. sp. has an unique 8/9–2–4 cirrus spiral formula. The length of the cirrus and its spriral formula 5–2–3 make E. mashegoi n. sp. morphometrically unique from other Enterogyrus spp. Sequences of the nuclear ribosomal DNA partial 18S and internal transcribed spacer (ITS1) and 28S were obtained and compared with available sequences of Enterogyrus in GenBank. This is the first record of data on 18S rDNA region of Enterogyrus spp. A phylogenetic comparison was conducted, which included all data available for Enterogyrus spp., but this was limited to 28S data. The closest species to both new species of the present study was an undescribed Enterogyrus sp. 2 from Sarotherodon galilaeus (Linnaeus) described from Senegal (i.e. 2.1 and 2.9% uncorrected pairwise genetic distance to E. multispiralis n. sp. and E. mashegoi n. sp., respectively). These species represent new records for Africa resulting in a total number of 12 described valid species.

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Acknowledgements

We are grateful to the staff of Nwanedi Reserve for their hospitality and the former manager, Natasha du Plessis for authorizing this study. We acknowledge Hendrik Hattingh, Johan Theron, Willem Smit and Esau Mbokane for their field and laboratory support. The authors would like to thank the Department of Botany and Zoology, Faculty of Science, Masaryk University of Brno for providing facilities available for the molecular analysis. This is contribution number XX from the NWU-Water Research Group.

Funding

The Division for Research Administration and Development, University of Limpopo, provided additional funding and supported administering of funds. IP was supported by the Czech Science Foundation, no. GBP505/12/G112. This work is based on the research supported by the South African Research Chairs Initiative of the Department of Science and Innovation and National Research Foundation of South Africa (Grant No 101054).

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Correspondence to W. J. Luus-Powell.

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A permit for the collection of fish was obtained from the Department of Economic Development and Tourism, Limpopo Province, approval number ZA/LP/HO/3370. This study was approved by the Faculty Higher Degree Committee of the University of Limpopo prior to fish collection and comply to all national animal ethical standards.

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Luus-Powell, W.J., Madanire-Moyo, G.N., Matla, M.M. et al. Monogenean parasites from the stomach of Oreochromis mossambicus from South Africa: two new species of Enterogyrus (Dactylogyridae: Ancyrocephalinae). Parasitol Res 119, 1505–1514 (2020). https://doi.org/10.1007/s00436-020-06650-2

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Keywords

  • Limpopo Province
  • Monogenea
  • Mozambique tilapia
  • Phylogenetic
  • Sequencing