Molecular characterisation of five Sarcocystis species in domestic sheep (Ovis aries) from Spain

Gjerde, Bjørn; de la Fuente, Concepción; Alunda, José María; Luzón, Mónica

doi:10.1007/s00436-019-06504-6

Protozoology - Original Paper
Published: 16 November 2019

Molecular characterisation of five Sarcocystis species in domestic sheep (Ovis aries) from Spain

Bjørn Gjerde ORCID: orcid.org/0000-0001-5011-1516¹,
Concepción de la Fuente²,
José María Alunda² &
Mónica Luzón²

Parasitology Research volume 119, pages 215–231 (2020)Cite this article

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Abstract

The major aim of the present study was to determine by molecular methods whether the wide and narrow types of macroscopic sarcocysts in Spanish sheep belonged to different species, that is, Sarcocystis gigantea and Sarcocystis medusiformis, respectively. Additionally, we wanted to identify and characterize molecularly the species forming microscopic sarcocysts and determine the phylogenetic placement of all species found. Portions of the oesophagus, diaphragm and hind legs containing macroscopic sarcocysts were collected from slaughtered culled ewes at an abattoir in the Province of Madrid, Central Spain, but both macroscopic and microscopic sarcocysts were isolated for molecular examination. Genomic DNA from 63 sarcocysts (21 macroscopic, 42 microscopic) were examined at the cytochrome c oxidase subunit I gene (cox1), while selected isolates of each species found were further examined at the 18S and 28S ribosomal RNA (rRNA) genes. The 63 sarcocysts comprised five cox1 sequence types, each corresponding to a particular sarcocyst type, and thus represented five Sarcocystis spp. The slender fusiform and thick macrocysts belonged to S. medusiformis and S. gigantea, respectively. The microscopic sarcocysts belonged to Sarcocystis arieticanis, Sarcocystis tenella and a Sarcocystis mihoensis-like species with slanting thorn-like cyst wall protrusions, which was characterised molecularly for the first time. Based on its phylogenetic position, the S. mihoensis-like species probably uses corvids as definitive hosts.

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Acknowledgments

We are indebted to the veterinary services and staff of the abattoir in Villarejo de Salvanés (Madrid). Their kind collaboration made possible the sampling of sheep carcasses.

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Department of Food Safety and Infection Biology, Faculty of Veterinary Medicine, Norwegian University of Life Sciences, P.O. Box 369 Sentrum, 0102, Oslo, Norway
Bjørn Gjerde
Department of Animal Health, Veterinary Faculty, Complutense University of Madrid, Avenida de Puerta de Hierro s/n, 28040, Madrid, Spain
Concepción de la Fuente, José María Alunda & Mónica Luzón

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Bjørn Gjerde
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Concepción de la Fuente
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José María Alunda
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Mónica Luzón
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Correspondence to Bjørn Gjerde.

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The muscle samples used in the present study were collected from the carcasses of sheep slaughtered according to EU regulations at an abattoir in Villarejo de Salvanés, Province of Madrid.

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Gjerde, B., de la Fuente, C., Alunda, J.M. et al. Molecular characterisation of five Sarcocystis species in domestic sheep (Ovis aries) from Spain. Parasitol Res 119, 215–231 (2020). https://doi.org/10.1007/s00436-019-06504-6

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Received: 09 August 2019
Accepted: 11 October 2019
Published: 16 November 2019
Issue Date: January 2020
DOI: https://doi.org/10.1007/s00436-019-06504-6

Keywords

Sarcocystis arieticanis
Sarcocystis tenella
Sarcocystis gigantea
Sarcocystis medusiformis
Sarcocystis mihoensis
Spain
18S rRNA gene
28S rRNA gene
Cytochrome c oxidase subunit I gene
Phylogeny