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Immunodiagnosis of human neurocysticercosis: comparative performance of serum diagnostic tests in Mexico

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Abstract

Immunodiagnosis has a supportive role in the diagnosis of neurocysticercosis (NCC). The aim of this study was to compare the validity of seven immunodiagnostic tests among serum samples from 58 patients with NCC, 26 patients with neurological diseases other than NCC, and 15 healthy controls. One test for viable parasite detection (HP10 antigen assay) and six for antibody detection were evaluated. For the entire sample, sensitivities ranged from 55.2% (NOVALISA) to 81.0% (enzyme-linked immunosorbent assay [ELISA] Taenia solium antibody), with the sensitivity of the latter test significantly higher than that of the in-house ELISA Taenia crassiceps, NOVALISA, enzyme-linked immunoelectrotransfer blot (EITB) CDC, and HP10. Overall, specificities were high, ranging from 85.4% (ELISA Ts) to 97.1% (NOVALISA), with no statistically significant differences. Detection of HP10 antigen was significantly associated with the presence of vesicular parasites. The simple and low-cost ELISA Taenia solium antibody Ab instead of EITB is recommended to support NCC diagnosis in both rural and hospital settings in Mexico.

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Funding

This work was partially supported by the “Programa Iberoamericano de Ciencia y TecnologÍa para el Desarrollo, CYTED” [211RT0432] and by the “Programa de Investigación para el Desarrollo y la Optimización de Vacunas, Inmunomoduladores y Métodos Diagnósticos del IIB” (PROVACADI, Instituto de Investigaciones Biomédicas, UNAM, México).

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Authors and Affiliations

  1. Departamento de Inmunología, Instituto de Investigaciones Biomédicas, UNAM, Ciudad de México, Mexico

    Marisela Hernández & Edda Sciutto

  2. Administración Nacional de Laboratorios e Institutos de Salud (ANLIS) “Dr. Carlos Malbrán”, Buenos Aires, Argentina

    Osvaldo-Germán Astudillo & Gustavo Diego

  3. Instituto de Diagnóstico y Referencia Epidemiológicos (InDRE), Secretaria de Salud, Ciudad de México, Mexico

    Jorge-Luis de-la-Rosa-Arana, Antonio Meza-Lucas & Ricardo García-Rodea

  4. School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, SAR, Hong Kong

    Matthew-L. Romo

  5. Unidad de Neuroinflamación, Instituto de Investigaciones Biomédicas-UNAM/Instituto Nacional de Neurología y Neurocirugía/Facultad de Medicina—UNAM, Ciudad de México, Mexico

    Andrea Toledo & Agnès Fleury

  6. Instituto Gulbenkian de Ciência (IGC), Lisbon, Portugal

    R.-Michael-E. Parkhouse

  7. Instituto de Salud Carlos III, Majadahonda, Madrid, Spain

    Teresa Garate

  8. Neurocysticercosis Clinic, Instituto Nacional de Neurología y Neurocirugía, Ciudad de México, Mexico

    Agnès Fleury

Authors
  1. Marisela Hernández
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  2. Osvaldo-Germán Astudillo
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  3. Gustavo Diego
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  4. Jorge-Luis de-la-Rosa-Arana
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  5. Antonio Meza-Lucas
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  6. Ricardo García-Rodea
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  7. Matthew-L. Romo
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  8. Andrea Toledo
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  9. R.-Michael-E. Parkhouse
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  10. Teresa Garate
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  11. Edda Sciutto
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  12. Agnès Fleury
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Contributions

This study was designed, supervised, and coordinated by RMEP, TG, ES, and AF. MH, OGA, GD, JLRA, AML, RGR, and AT carried out the clinical assessment and the immunoassays; MLR and AF carried out the analysis and interpretation of these data. JLRA, RMEP, ES, and AF drafted the manuscript. All authors critically revised the manuscript for intellectual content, read, and approved the final manuscript. ES and AF are guarantors of the paper.

Corresponding author

Correspondence to Agnès Fleury.

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The authors declare that they have no conflict of interest.

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Section Editor: David Bruce Conn

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Dr. Teresa Garate passed away before the submission of the final version of this manuscript.

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Hernández, M., Astudillo, OG., Diego, G. et al. Immunodiagnosis of human neurocysticercosis: comparative performance of serum diagnostic tests in Mexico. Parasitol Res 118, 2891–2899 (2019). https://doi.org/10.1007/s00436-019-06425-4

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