Characterization of the complete mitochondrial genome of the echinostome Echinostoma miyagawai and phylogenetic implications
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Echinostomes are important intestinal foodborne parasites. Despite their significance as pathogens, characterization of the molecular biology and phylogenetics of these parasites are limited. In the present study, we determined the entire mitochondrial (mt) genome of the echinostome Echinostoma miyagawai (Hunan isolate) and examined the phylogenetic relationship with selected members of the suborder Echinostomata. The complete mt genome of E. miyagawai (Hunan isolate) was 14,468 bp in size. This circular mt genome contained 12 protein-coding genes, 22 transfer RNA genes, two ribosomal RNA genes, and one non-coding region. The gene order and genomic content were identical with its congeners. Phylogenetic analyses (maximum parsimony, maximum likelihood, and Bayesian inference) based on the concatenated amino acid sequences of 12 protein-coding genes strongly supported monophyly for the genus Echinostoma; however, they rejected monophyly for the family Echinostomatidae and the genus Fasciola. The mt genomic data described in this study provides useful genetic markers for studying the population genetics, molecular biology, and phylogenetics of these echinostomes.
KeywordsEchinostome Mitochondrial genome Mitochondrial DNA Phylogenetic analyses
This study was financially supported by the Planned Program of Hunan Province Science and Technology Innovation (Grant no. 2018RS3085) and the Training Program for Excellent Young Innovators of Changsha (Grant no. KQ1802035).
Compliance with ethical standards
All procedures involving animals in the present study were approved, and this study was approved by the Animal Ethics Committee of Hunan Agricultural University (No. 43321503).
The authors declared that they have no competing interests.
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