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Parasitology Research

, Volume 118, Issue 9, pp 2723–2727 | Cite as

A case of bovine trypanosomiasis caused by Trypanosoma theileri in Sicily, Italy

  • Benedetta Amato
  • Francesco Mira
  • Vincenzo Di Marco Lo Presti
  • Annalisa Guercio
  • Laura Russotto
  • Francesca Gucciardi
  • Maria Vitale
  • Antonino Lena
  • Guido Ruggero Loria
  • Roberto Puleio
  • Vincenza CannellaEmail author
Protozoology - Short Communication

Abstract

Despite some researchers reporting clinical signs in cattle associated with Trypanosoma theileri, its role as a pathogen is still unclear. We describe here the isolation of Trypanosoma theileri during a routine laboratory investigation. Mature and immature vital parasitic forms were observed within hematopoietic cell cultures from the bone marrow of one cow for monocyte isolation. The animal was submitted to clinical examination and blood sample counting (CBC). Postmortem analysis included gross and histological examination and PCR in the liver, spleen, brain, lymph nodes, and lungs. PCR and Giemsa staining were used for parasite identification. A second cow belonging to the same farm was positive for Trypanosoma theileri by PCR performed on blood sample. In this case, the postmortem analysis included also testis. Clinical examination showed only a reduction in body weight in both cases. The CBC revealed an increase of lymphocytes and neutrophils while red blood cells were within the normal range. Spleen was slightly increased in volume and the histology revealed a proliferative activity of the white and red pulp. The biomolecular analysis identified the parasite as Trypanosoma theileri and its DNA was detected in the bone marrow, testis, and brain. The unusual finding of parasite in the brain, testis, and bone marrow raises new clinical implication on disease course and also possible sexual transmission.

Keywords

Trypanosoma Cattle Bone marrow Phylogeny 

Notes

Acknowledgments

The authors thank Emanuela Leonardi, Elena Rappazzo, and Giovanna Romeo for their technical support.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

References

  1. Braun U, Rogg E, Walser M, Nehrbass D, Guscetti F, Mathis A, Deplazes P (2002) Trypanosoma theileri in the cerebrospinal fluid and brain of a heifer with suppurative meningoencephalitis. Vet Rec 150(1):18–19CrossRefGoogle Scholar
  2. Cox A, Tilley A, McOdimba F, Fyfe J, Eisler M, Hide G, Welburn S (2005) A PCR based assay for detection and differentiation of African trypanosome species in blood. Exp Parasitol 111:24–29CrossRefGoogle Scholar
  3. D’Avila-Levy CM, Boucinha C, Kostygov A, Santos HL, Morelli KA, Grybchuk-Ieremenko A, Duval L, Votýpka J, Yurchenko V, Grellier P, Lukeš J (2015) Exploring the environmental diversity of kinetoplastid flagellates in the high-throughput DNA sequencing era. Mem Inst Oswaldo Cruz 110(8):956–965.  https://doi.org/10.1590/0074-02760150253 CrossRefGoogle Scholar
  4. Doherty ML, Windle H, Vooreis HP, Larkin H, Casey M, Clery D, Murray M (1993) Clinical disease associated with Trypanosoma theileri infection in a calf in Ireland. Vet Rec 132:653–656CrossRefGoogle Scholar
  5. Fraga J, Fernández-Calienes A, Montalvo AM, Maes I, Deborggraeve S, Büscher P, Dujardin JC, Van der Auwera G (2016) Phylogenetic analysis of the Trypanosoma genus based on the heat-shock protein 70 gene. Infect Genet Evol 43:165–172.  https://doi.org/10.1016/j.meegid.2016.05.016 CrossRefGoogle Scholar
  6. Geysen D, Delespaux V, Geerts S (2003) PCR-RFLP using Ssu-rDNA amplification as an easy method for species-specific diagnosis of Trypanosoma species in cattle. Vet Parasitol 110:171–180CrossRefGoogle Scholar
  7. Greco A, Loria GR, Dara S, Luckins T, Sparagano O (2000) First isolation of Trypanosoma theileri in sicilian cattle. Vet Res Commun 24:471–475.  https://doi.org/10.1023/A:1006403706224 CrossRefGoogle Scholar
  8. Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl Acids Symp Ser 41:95–98Google Scholar
  9. Hoare CA (1972) Subgenus Megatrypanum. In: The trypanosome of mammals: a zoological monograph. Blackwell Scientific Publications, Oxford, pp 123–141Google Scholar
  10. Hutchinson R, Stevens JR (2018) Barcoding in trypanosomes. Parasitology 145(5):563–573.  https://doi.org/10.1017/S0031182017002049 CrossRefGoogle Scholar
  11. Kelly S, Ivens A, Mott GA, O’Neill E, Emms D, Macleod O, Voorheis P, Tyler K, Clark M, Matthews J, Matthews K, Carrington M (2017) An alternative strategy for trypanosome survival in the mammalian bloodstream revealed through genome and transcriptome analysis of the ubiquitous bovine parasite Trypanosoma (Megatrypanum) theileri. Genome Biol Evol 9(8):2093–2109.  https://doi.org/10.1093/gbe/evx152 CrossRefGoogle Scholar
  12. Kumar S, Stecher G, Tamura K (2016) MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 33(7):1870–1874.  https://doi.org/10.1093/molbev/msw054 CrossRefGoogle Scholar
  13. Lanevschi-Pietersma A, Ogunremi O, Desrocher A (2004) Parasitemia in a neonatal bison calf. Vet Clin Pathol 33(3):173–176CrossRefGoogle Scholar
  14. Latif AA, Bakheit MA, Mohamed AE, Zweygarth E (2004) High infection rates of the tick Hyalomma anatolicum anatolicum with Trypanosoma theileri. Onderstepoort J Vet Res 71:251–256CrossRefGoogle Scholar
  15. Polodori GA, Grelloni V, Moretti A, Ciurnelli M, Principato M (1982) Trypanosoma theileri infection in cattle in central Italy. Atti della Società Italiana delle Scienze Veterinarie 36:650–652Google Scholar
  16. Rodriguez AC, Campaner M, Takata CS, Dell’ Porto A, Milder RV, Takeda GF, Teixeira MM (2003) Brazilian isolates of Trypanosoma (Megatrypanum) theileri: diagnosis and differentiation of isolates from cattle and water buffalo based on biological characteristics and randomly amplified DNA sequences. Vet Parasitol 116:185–207CrossRefGoogle Scholar
  17. Soltysiak Z, Gorczykowski M, Pawlas-Opiela M, Chelmonska-Soyra A, Nowacki W (2009) Accidental discovery of Trypanosoma theileri in the in vitro culture of the heifer lymphocytes. Pol J Vet Sci 12(3):395–398Google Scholar
  18. Sood NK, Singla LD, Singh RS, Uppal SK (2011) Association of Trypanosoma theileri with peritonitis in a pregnant cross-bred cow: a case report. Vet Med-Czech 56(2):82–84CrossRefGoogle Scholar
  19. Trouplin V, Boucherit N, Gorve L, Conti F, Mottola G, Ghigo E (2013) Bone marrow-derived macrophage production. JoVE 81:1–6Google Scholar
  20. Villa A, Gutierrez C, Gracia E, Moreno B, Chacon G, Sanz PV, Buscher P, Touratier L (2008) Presence of Trypanosoma theileri in Spanish cattle. Ann N Y Acad Sci 1149:352–354.  https://doi.org/10.1196/annals CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Benedetta Amato
    • 1
  • Francesco Mira
    • 1
  • Vincenzo Di Marco Lo Presti
    • 1
  • Annalisa Guercio
    • 1
  • Laura Russotto
    • 1
  • Francesca Gucciardi
    • 1
  • Maria Vitale
    • 1
  • Antonino Lena
    • 1
  • Guido Ruggero Loria
    • 1
  • Roberto Puleio
    • 1
  • Vincenza Cannella
    • 1
    Email author
  1. 1.Istituto Zooprofilattico Sperimentale della Sicilia “A. Mirri”PalermoItaly

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