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Seasonal dynamics of a population of Phlebotomus (Larroussius) perfiliewi Parrot, 1930 (Diptera: Psychodidae) in North-Eastern Romania

  • Arthropods and Medical Entomology - Original Paper
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Abstract

Sand flies were collected in a location from Romania in order to estimate their abundance and seasonal variation in correlation with environmental and anthropic factors. From May to October 2017, eight premises with different animal species were sampled for sand flies in a household from Fundătura village, Vaslui County, in North-Eastern Romania. Animal-related data, shelter-related data, and climatic parameters were recorded. All (n = 150) collected sand flies were Phlebotomus perfiliewi. A mono-modal type of abundance trend has been recorded (a single peak at the beginning of August). The first day of capture was in mid-July. The total number of females during the peak season was significantly higher than the total number of males. The highest percentage of males was recorded at the beginning and at the end of the sand fly activity. Only the traps placed in the poultry enclosure built from clay and wood were positive. A strong positive correlation was recorded between the total number of collected sand flies and the minimum and the maximum temperature. The analysis of the climatic data shows that the first presence of sand flies was registered only after the average minimum temperature for the previous 7 days was above 15 °C.

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References

  • Alten B, Maia C, Afonso MO, Campino L, Jiménez M, González E, Molina R, Bañuls AL, Prudhomme J, Vergnes B, Toty C, Cassan C, Rahola N, Thierry M, Sereno D, Bongiorno G, Bianchi R, Khoury C, Tsirigotakis N, Dokianakis E, Antoniou M, Christodoulou V, Mazeris A, Karakus M, Ozbel Y, Arserim SK, Erisoz Kasap O, Gunay F, Oguz G, Kaynas S, Tsertsvadze N, Tskhvaradze L, Giorgobiani E, Gramiccia M, Volf P, Gradoni L (2016) Seasonal dynamics of phlebotomine sand fly species proven vectors of Mediterranean leishmaniasis caused by Leishmania infantum. PLoS Negl Trop Dis 10(2):e0004458

    Article  PubMed  PubMed Central  Google Scholar 

  • Belen A, Alten B (2005) Variation in life table characteristics among populations of Phlebotomus papatasi at different altitudes. J Vector Ecol 31:35–44

    Article  Google Scholar 

  • Belen A, Alten B, Aytekin A (2004) Altitudinal variation in morphometric and molecular characteristics of Phlebotomus papatasi populations. Med Vet Entomol 18:343–350

    Article  PubMed  CAS  Google Scholar 

  • Bogdan C, Schonian G, Banuls AL, Hide M, Pratlong F, Lorenz E, Röllinghoff M, Mertens R (2001) Visceral leishmaniasis in a German child who had never entered a known endemic area: case report and review of the literature. Clin Infect Dis 32:302–306

    Article  PubMed  CAS  Google Scholar 

  • Boutsini S, Athanasiou LV, Spanakos G, Ntousi D, Dotsika E, Bisia M, Papadopoulos E (2017) Phlebotomine sandflies and factors associated with their abundance in the leishmaniasis endemic area of Attiki, Greece. Parasitol Res 117:107–113

    Article  PubMed  Google Scholar 

  • Chaskopoulou A, Giantsis IA, Demir S, Bon MC (2016) Species composition, activity patterns and blood meal analysis of sand fly populations (Diptera: Psychodidae) in the metropolitan region of Thessaloniki, an endemic focus of canine leishmaniasis. Acta Trop 158:170–176

    Article  PubMed  Google Scholar 

  • Copăceanu P, Motilică V, Nicolaescu N, Iliescu I (1955) The first case of visceral leishmaniasis in adults in RPR. Rev Ig Microbiol Epid 3:88–90 (In Romanian)

    Google Scholar 

  • Dancesco P (2008) Species of sandflies (Diptera: Psychodidae) in Romania, some aspects of their ecology and new capture stations. Trav Mus Nat Hist Grigore Antipa 51:185–199 (In French)

    Google Scholar 

  • Dumitrache MO, Nachum-Biala Y, Gilad M, Mircean V, Cazan CD, Mihalca AD, Baneth G (2016) The quest for canine leishmaniasis in Romania: the presence of an autochthonous focus with subclinical infections in an area where disease occurred. Parasit Vectors 9:297

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • European Centre for Disease Prevention and Control; European Food Safety Authority (2018) Field sampling methods for mosquitoes, sandflies, biting midges and ticks – VectorNet project 2014–2018. ECDC and EFSA, Stockholm and Parma

    Google Scholar 

  • Gálvez R, Descalzo MA, Miró G, Jiménez MI, Martín O, Dos Santos-Brandao F, Guerrero I, Cubero E, Molina R (2010) Seasonal trends and spatial relations between environmental/meteorological factors and leishmaniosis sand fly vector abundances in Central Spain. Acta Trop 115:95–102

    Article  PubMed  Google Scholar 

  • Gogoaşe MG, Teodorescu I, Preda C, Ionescu SC (2013) Two case reports on visceral leishmaniasis diagnosed in Romania. Roum Arch Microbiol Immunol 72:49–62

    PubMed  Google Scholar 

  • González E, Jiménez M, Hernández S, Martín-Martín I, Molina R (2017) Phlebotomine sand fly survey in the focus of leishmaniasis in Madrid, Spain (2012–2014): seasonal dynamics, Leishmania infantum infection rates and blood meal preferences. Parasit Vectors 10:368

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • Ielenicz M, Pătru I (2005) Physical geography of Romania, Editura Universitarã, Bucharest, ISBN 973-7787-37, pp7 (in Romanian)

  • Ivovic V, Patakakis M, Tselentis Y, Chaniotis B (2007) Faunistic study of sandflies in Greece. Med Vet Entomol 21:121–124

    Article  PubMed  CAS  Google Scholar 

  • Kasap OE, Alten B (2005) Laboratory estimation of degree-day developmental requirements of Phlebotomus papatasi (Diptera: Psychodidae). J Vector Ecol 30:328–333

    PubMed  Google Scholar 

  • Kasap OE, Belen A, Kaynas S, Simsek FM, Biler L, Ata N, Alten B (2009) Activity patterns of sand fly (Diptera: Psychodidae) species and comparative performance of different traps in an endemic cutaneous leishmaniasis focus in Cukurova Plain, Southern Anatolia, Turkey. Acta Vet Brno 78:327–335

    Article  Google Scholar 

  • Killick-Kendrick R (1999) The biology and control of phlebotomine sand flies. Clin Dermatol 17:279–289

    Article  PubMed  CAS  Google Scholar 

  • Lewis DJ (1982) A taxonomic review of the genus Phlebotomus (Diptera: Psychodidae). Bullbr Musnat Hist Entomol 45:171–209

    Google Scholar 

  • Lisi O, D’Urso V, Vaccalluzzo V, Bongiorno G, Khoury C, Severini F, Di Muccio T, Gramiccia M, Gradoni L, Maroli M (2014) Persistence of phlebotomine Leishmania vectors in urban sites of Catania (Sicily, Italy). Parasit Vectors 9:56032

    Google Scholar 

  • Lupașcu G, Ciplea A, Gherman I (1957) Contributions to the study of the reservoir of infection in the outbreak of leishmaniasis in Craiova region. Stud Cercet Inframicrobiol Parzitol 8:647–651 (In Romanian)

    Google Scholar 

  • Lupașcu G, Bossie A, Ciplea A, Costin P (1968) Research on the animal reservoir of Leishmania. Arch Roum Pathol Exp Microbiol 28:29–35 (In French)

    Google Scholar 

  • Maia C, Afonso MO, Neto L, Dionísio L, Campino L (2009) Molecular detection of Leishmania infantum in naturally infected Phlebotomus perniciosus from Algarve region, Portugal. J Vector Borne Dis 46:268–272

    PubMed  CAS  Google Scholar 

  • Manicatide N (1919-1920) Two cases of Kala-Azar observed in Romania. Bull Sect Sci Acad Roum:105 (In French)

  • Maroli M, Bettini S (1977) Leishmaniasis in Tuscany (Italy): (I) an investigation on phlebotomine sandflies in Grosseto Province. Trans R Soc Trop Med Hyg 71:315–321

    Article  PubMed  CAS  Google Scholar 

  • Maroli M, Rossi L, Baldelli R, Capelli G, Ferroglio E, Genchi C, Gramiccia M, Mortarino M, Pietrobelli M, Gradoni L (2008) The northward spread of leishmaniasis in Italy: evidence from retrospective and ongoing studies on the canine reservoir and phlebotomine vectors. Tropical Med Int Health 13:256–264

    Article  Google Scholar 

  • Maroli M, Feliciangeli MD, Bichaud L, Charrel RN, Gradoni L (2013) Phlebotomine sandflies and the spreading of leishmaniases and other diseases of public health concern. Med Vet Entomol 27:123–147

    Article  PubMed  CAS  Google Scholar 

  • Mihăilescu M, Niciloff D (1934) Two cases of spontaneous canine leishmaniasis in Romania. Arhiva Vet 26:43–53 (In Romanian)

    Google Scholar 

  • Minculescu M, Bîrzu I, Crețu S, Iovănescu F, Ionescu D, Lupulescu V et al (1955) Reflections on the first outbreak of infantile leishmaniasis identified RPR. Stud Cercet Inframicrobiol Parazitol III 6:596–603 (In Romanian)

    Google Scholar 

  • Mircean V, Dumitrache MO, Mircean M, Bolfa P, Györke A, Mihalca AD (2014) Autochthonous canine leishmaniasis in Romania: neglected or (re) emerging. Parasit Vectors 7:135

    Article  PubMed  PubMed Central  Google Scholar 

  • Neghina R, Neghina AM, Merkler C, Marincu I, Moldovan R, Iacobiciu I (2009) Importation of visceral leishmaniasis in returning Romanian workers from Spain. Travel Med Infect Dis 7:35–39

    Article  PubMed  Google Scholar 

  • Oguz G, Kasap OE, Alten B (2017) Wing morphology variations in a natural population of Phlebotomus tobbi Adler and Theodor 1930. J Vector Ecol 42:223–232

    Article  PubMed  Google Scholar 

  • Parham PE, Waldock J, Christophides GK, Hemming D, Agusto F, Evans KJ, Fefferman N, Gaff H, Gumel A, LaDeau S, Lenhart S, Mickens RE, Naumova EN, Ostfeld RS, Ready PD, Thomas MB, Velasco-Hernandez J, Michael E (2015) Climate, environmental and socio-economic change: weighing up the balance in vector-borne disease transmission. Philos Trans R Soc Lond Ser B Biol Sci 370:20130551

    Article  Google Scholar 

  • Pavel G, Timofte D, Mocanu D, Malancus R, Solcan C (2017) Imported leishmaniasis in a dog in a sandfly-populated area in northeastern Romania. J Vet Diagn Investig 29:683–685

    Article  Google Scholar 

  • Prudhomme J, Rahola N, Toty C, Cassan C, Roiz D, Vergnes B, Thierry M, Rioux JA, Alten B, Sereno D, Bañuls AL (2015) Ecology and spatiotemporal dynamics of sandflies in the Mediterranean Languedoc region (Roquedur area, Gard, France). Parasit Vectors 8:1–14

    Article  CAS  Google Scholar 

  • Rioux JA, Carron S, Dereure J, Perieres J, Zeraia L, Franquet E, Babinot M, Gállego M, Prudhomme J (2013) Ecology of leishmaniasis in the South of France. 22. Reliability and representativeness of 12 Phlebotomus ariasi, P. perniciosus and Sergentomyia minuta (Diptera: Psychodidae) sampling stations in Vallespir (eastern French Pyrenees region). Parasite 20:34

    Article  PubMed  PubMed Central  Google Scholar 

  • Risueño J, Muñoz C, Pérez-Cutillas P, Goyena E, Gonzálvez M, Ortuño M, Bernal LJ, Ortiz J, Alten B, Berriatua E (2017) Understanding Phlebotomus perniciosus abundance in south-east Spain: assessing the role of environmental and anthropic factors. Parasit Vectors 10:189

    Article  PubMed  PubMed Central  Google Scholar 

  • Romanian National Institute of Statistics (2013) Final results of population census from 2011. Table 8, Ethnic stable population - counties, municipalities, towns, communes. http://www.recensamantromania.ro/wp-content/uploads/2013/07/sR_Tab_8.xls. Accessed 5 September 2018 (in Romanian)

  • Rossi E, Bongiorno G, Ciolli E, Di Muccio T, Scalone A, Gramiccia M, Gradoni L, Maroli M (2008) Seasonal phenology, host-blood feeding preferences and natural Leishmania infection of Phlebotomus perniciosus (Diptera, Psychodidae) in a high-endemic focus of canine leishmaniasis in Rome province, Italy. Acta Trop 105:158–165

    Article  PubMed  CAS  Google Scholar 

  • Sawalha SS, Ramlawi A, Sansur RM, Salem IM, Amr ZS (2017) Diversity, ecology, and seasonality of sand flies (Diptera: Psychodidae) of the Jenin District (Palestinian territories). J Vector Ecol 42:120–129

    Article  PubMed  Google Scholar 

  • Simsek FM, Alten B, Caglar SS, Ozbel Y, Aytekin AM, Kaynas S, Belen A, Kasap OE, Yaman M, Rastgeldi S (2007) Distribution and altitudinal structuring of phlebotomine sand flies (Diptera: Psychodidae) in southern Anatolia, Turkey: their relation to human cutaneous leishmaniasis. J Vector Ecol 32:269–279

    Article  PubMed  Google Scholar 

  • Tanase OI, Daraban C, Velescu E, Boghean D, Bocaneti-Daraban F (2018) Symptomatic Leishmaniasis in an Italian Segugio dog from northeastern Romania: a case report. Iran J Parasitol 13:673–678

    PubMed  PubMed Central  Google Scholar 

  • Tánczos B, Balogh N, Király L, Biksi I, Szeredi L, Gyurkovsky M, Scalone A, Fiorentino E, Grammiccia M, Farkas R (2012) First record of autochtonous canine leishmaniasis in Hungary. Vector Borne Zoonotic Dis 12:588–594

    Article  PubMed  PubMed Central  Google Scholar 

  • Tarallo VD, Dantas-Torres F, Lia RP, Otranto D (2010) Phlebotomine sand fly population dynamics in a leishmaniasis endemic peri-urban area in southern Italy. Acta Trop 116:227–234

    Article  PubMed  Google Scholar 

  • Volf P, Ozbel Y, Akkafa F, Svobodová M, Votýpka J, Chang KP (2002) Sand flies (Diptera: Phlebotominae) in Sanliurfa, Turkey: relationship of Phlebotomus sergenti with the epidemic of anthroponotic cutaneous leishmaniasis. J Med Entomol 39:12–15

    Article  PubMed  CAS  Google Scholar 

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Funding

This study was supported from the following project: VectorNet, a European network for sharing data on the geographic distribution of arthropod vectors, transmitting human and animal disease agents (contract OC/EFSA/AHAW/2013/02-FWC1) funded by the European Food Safety Authority (EFSA) and the European Centre for Disease Prevention and Control (ECDC) and PCCDI 57/2018 (UEFISCDI). The training of CDC was done under the frame of EurNegVec COST TD1303 Action, and PN-III-P1-1.1-MC-2017-1287 (UEFISCDI).

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Correspondence to Cristina Daniela Cazan.

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This article does not contain any studies with human participants or animals performed by any of the authors. Additional informed consent was obtained from all individual participants for whom identifying information is included in this article.

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Online Resource 1

Environmental data for each day of the study (XLSX 18 kb)

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Trapping days data (XLSX 10 kb)

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Cazan, C.D., Păstrav, I.R., Györke, A. et al. Seasonal dynamics of a population of Phlebotomus (Larroussius) perfiliewi Parrot, 1930 (Diptera: Psychodidae) in North-Eastern Romania. Parasitol Res 118, 1371–1384 (2019). https://doi.org/10.1007/s00436-019-06296-9

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