Skip to main content

Genetic analysis of Rhipicephalus sanguineus sensu lato ticks, parasites of dogs in the Canary Islands, Cyprus, and Croatia, based on mitochondrial 16S rRNA gene sequences

Abstract

The aim of this work was to perform an analysis based on mtDNA sequences of the 16S rRNA gene in order to determine the phylogenetic position of ticks belonging to the Rhipicephalus sanguineus group from the Canary Islands, Cyprus, and Croatia. All the haplotypes obtained from ticks collected in the Canary Islands and Croatia grouped with R. sanguineus sensu stricto from France, Portugal, Italy, Switzerland, Argentina, Chile, Uruguay, and the USA. The sequences of R. sanguineus sensu lato from Cyprus formed a clade with R. sanguineus s.l. from Egypt, Turkey, and Romania, which belongs to the “Rhipicephalus sp. morphotype I” or “southeastern European lineage.” Ticks determined as R. turanicus s.l. from Cyprus clustered separately from the remaining clades of the R. sanguineus group, including R. turanicus s.s. The data show that R. sanguineus s.s. is present in the Canary Islands and Croatia, while R. sanguineus “southeastern lineage” is found in Cyprus.

This is a preview of subscription content, access via your institution.

Fig. 1

References

  • Attipa C, Hicksa CAE, Barkera EN, Christodoulouc V, Neofytoub K, Mylonakisd ME, Siarkoue VI, Vingopouloue EI, Soutterf F, Chochlakisg D, Psaroulakig A, Papasouliotisa K, Tasker S (2017) Canine tick-borne pathogens in Cyprus and a unique canine case of multiple co-infections. Ticks Tick-Borne Dis 8:341–346

    Article  PubMed  PubMed Central  Google Scholar 

  • Burlini L, Texeira KRS, Szabó MPJ, Famadas KM (2010) Molecular dissimilarities of Rhipicephalus sanguineus (Acari: Ixodidae) in Brazil and its relation with samples throughout the world: is there a geographical pattern? Exp Appl Acarol 50:361–374

    Article  PubMed  Google Scholar 

  • Chitimia-Dobler L, Langguth J, Pfeffer M, Kattner S, Küpper T, Friese D, Dobler G, Guglielmone AA, Nava S (2017) Genetic analysis of Rhipicephalus sanguineus sensu lato ticks parasites of dogs in Africa north of the Sahara based on mitochondrial DNA sequences. Vet Parasitol 239:1–6

    Article  PubMed  CAS  Google Scholar 

  • Chochlakis D, Ioannou I, Sandalakis V, Dimitriou T, Kassinis N, Papadopoulos B, Tselentis Y, Psaroulaki A (2012) Spotted fever group rickettsiae in ticks in Cyprus. Microb Ecol 63:314–323

    Article  PubMed  Google Scholar 

  • Dantas-Torres F, Otranto D (2015) Further thoughts on the taxonomy and vector role of Rhipicephalus sanguineus group ticks. Vet Parasitol 208:9–13

    Article  PubMed  Google Scholar 

  • Dantas-Torres F, Latrofa MS, Annoscia G, Gianelli A, Parisi A, Otranto D (2013) Morphological and genetic diversity of Rhipicephalus sanguineus sensu lato from the New and Old Worlds. Parasit Vectors 6:213

    Article  PubMed  PubMed Central  Google Scholar 

  • Dantas-Torres F, Maia C, Latrofa MS, Annoscia G, Otranto D (2017) Genetic characterization of Rhipicephalus sanguineus (sensu lato) ticks from dogs in Portugal. Parasit Vectors 10:133

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Dantas-Torres F, Latrofa MS, Ramos RAN, Lia RP, Capelli G, Parisi A, Porretta D, Urbanelli S, Otranto D (2018) Biological compatibility between two temperate lineages of brown dog tick, Rhipicephalus sanguineus (sensu lato). Parasit Vectors 11:398

    Article  PubMed  PubMed Central  Google Scholar 

  • Eremeeva ME, Zambrano ML, Anaya L, Beati L, Karpathy SE, Santos-Silva MM, Salceda B, MacBeth D, Olguin H, Dasch GA, Aranda CA (2011) Rickettsia rickettsii in Rhipicephalus ticks. J Med Entomol 48:418–421

    Article  PubMed  Google Scholar 

  • Estrada-Peña A, Bouattour A, Camicas JL, Walker AR (2004) Ticks of domestic animals in the Mediterranean Region: a guide to identification of species. University of Zaragoza, Zaragoza, p 133

    Google Scholar 

  • Hall TA (1999) BioEdit: a user friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98

    CAS  Google Scholar 

  • Hekimoglu O, Saglam IK, Ozer N, Estrada-Peña A (2016) New molecular data shed light on the global phylogeny and species limits of the Rhipicephalus sanguineus complex. Ticks Tick-Borne Dis 7:798–897

    Article  PubMed  Google Scholar 

  • International Commission on Zoological Nomenclature (1999) International code of zoological nomenclature, fourth edn. International Trust for Zoological Nomenclature, London, p 156

  • Jones EO, Gruntmeir JM, Hamer SA, Little SE (2017) Temperate and tropical lineages of brown dog ticks in North America. Vet Parasitol Reg Stud Rep 7:58–61

    Google Scholar 

  • Labruna MB, Gerardi M, Krawczak FS, Moraes-Filho J (2017) Comparative biology of the tropical and temperate species of Rhipicephalus sanguineus sensu lato (Acari: Ixodidae) under different laboratory conditions. Ticks Tick-Borne Dis 8:146–156

    Article  PubMed  Google Scholar 

  • Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA, McWilliam H, Valentin F, Wallace IM, Wilm A, Lopez R, Thompson JD, Gibson TJ, Higgins DG (2007) Clustal W and Clustal X version 2.0. Bioinformatics 23:2947–2948

    Article  PubMed  CAS  Google Scholar 

  • Le Fiche PD, Altan Y, Campbell JB, Efstateuou GC (1974) Ticks (Ixodoidea) of domestic animals in Cyprus. Bull Entomol Res 64:53–63

    Article  Google Scholar 

  • Levin ML, Studer E, Killmaster L, Zemtsova G, Mumcuoglu KY (2012) Crossbreeding between different geographical populations of the brown dog tick, Rhipicephalus sanguineus (Acari: Ixodidae). Exp Appl Acarol 58:51–68

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Liu GH, Chen YZ, Song HQ, Lin RQ, Zhou DH, Zhu XQ (2013) Complete mitochondrial genome sequence data provides evidence that dog tick Rhipicephalus sanguineus (Acari: Ixodidae) represents a species complex. Int J Biol Sci 9:361–369

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Mangold AJ, Bargues MD, Mas-Coma S (1998) Mitochondrial 16S rRNA sequences and phylogenetic relationships of Rhipicephalus and other tick genera among Metastriata (Acari: Ixodidae). Parasitol Res 84:478–484

    Article  PubMed  CAS  Google Scholar 

  • Moraes-Filho J, Marcili A, Nieri-Bastos F, Richtzenhain LJ, Labruna MB (2011) Genetic analysis of ticks belonging to the Rhipicephalus sanguineus group in Latin America. Acta Trop 117:51–55

    Article  PubMed  CAS  Google Scholar 

  • Nava S, Mastropaolo M, Venzal JM, Mangold AJ, Guglielmone AA (2012) Mitochondrial DNA analysis of Rhipicephalus sanguineus sensu lato (Acari: Ixodidae) in the Southern Cone of South America. Vet Parasitol 190:547–555

    Article  PubMed  CAS  Google Scholar 

  • Nava S, Estrada-Peña A, Petney T, Beati L, Labruna MB, Szabó MPJ, Venzal JM, Mastropaolo M, Mangold AJ, Guglielmone AA (2015) The taxonomic status of Rhipicephalus sanguineus (Latreille, 1806). Vet Parasitol 208:2–8

    Article  PubMed  Google Scholar 

  • Nava S, Beati L, Venzal JM, Labruna MB, Szabo MPJ, Petney TN, Saracho Bottero MN, Tarragona EL, Dantas-Torres F, Santos Silva MM, Mangold AJ, Guglielmone AA, Estrada-Peña A (2018) Rhipicephalus sanguineus (Latreille, 1806): Neotype designation, morphological re-description of all parasitic stages and molecular characterization. Ticks Tick-Borne Dis 9:1573–1585

    Article  PubMed  Google Scholar 

  • Pegram RG, Clifford CM, Walker JB, Keirans JE (1987) Clarification of the Rhipicephalus sanguineus group (Acari: Ixodidae). I. R. sulcatus Neumann, 1908 and R. turanicus Pomerantsev, 1936. Syst Parasitol 10:3–26

    Article  Google Scholar 

  • Sanches GS, Evora PM, Mangold AJ, Jittapalapong S, Rodríguez-Mallon A, Guzmán PEE, Bechara GH, Camargo-Mathias MI (2016) Molecular, biological, and morphometric comparisons between different geographical populations of Rhipicephalus sanguineus sensu lato (Acari: Ixodidae). Vet Parasitol 215:78–87

    Article  PubMed  CAS  Google Scholar 

  • Szabó MPJ, Mangold AJ, Joao CF, Bechara GH, Guglielmone AA (2005) Biological and DNA evidence of two dissimilar populations of the Rhipicephalus sanguineus tick group (Acari: Ixodidae) in South America. Vet Parasitol 130:131–140

    Article  PubMed  CAS  Google Scholar 

  • Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5 molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Walker JB, Keirans JE, Horak IG (2000) The genus Rhipicephalus (Acari, Ixodidae): a guide to the brown ticks of the world, Cambridge University Press, 643

  • Zemtsova G, Apanaskevich DA, Reeves WK, Hahn M, Snellgrove A, Levin M (2016) Phylogeography of Rhipicephalus sanguineus sensu lato and its relationships with climate factors. Exp Appl Acarol 69:191–203

    Article  PubMed  PubMed Central  Google Scholar 

Download references

Acknowledgements

Dr. Richard G. Robbins, Walter Reed Biosystematics Unit, Department of Entomology, Smithsonian Institution, Washington, DC, helpfully reviewed and commented on an earlier version of our manuscript.

Funding

Financial support to SN was provided by INTA, Asociación Cooperadora INTA Rafaela, and CONICET.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Lidia Chitimia-Dobler.

Ethics declarations

Conflict of interest

The authors declare that they have no competing interests.

Additional information

Handling Editor: Julia Walochnik

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Chitimia-Dobler, L., Kurzrock, L., Molčányi, T. et al. Genetic analysis of Rhipicephalus sanguineus sensu lato ticks, parasites of dogs in the Canary Islands, Cyprus, and Croatia, based on mitochondrial 16S rRNA gene sequences. Parasitol Res 118, 1067–1071 (2019). https://doi.org/10.1007/s00436-019-06214-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00436-019-06214-z

Keywords

  • Rhipicephalus sanguineus s.l.
  • Canary Islands
  • Cyprus
  • Croatia
  • Genetic analyses