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Chinese liver fluke Clonorchis sinensis infection changes the gut microbiome and increases probiotic Lactobacillus in mice

  • Helminthology - Short Communication
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Abstract

Chinese liver fluke Clonorchis sinensis changes the host’s immune system. Recently, it has been reported that helminths including C. sinensis can ameliorate immune-related diseases such as allergy. In addition, recent studies showed that helminth infection can alleviate immune-mediated disorders by altering the gut microbiome. However, changes in the gut microbiome due to C. sinensis have not been reported yet. In this study, changes in the gut microbiome of C57BL/6 mice infected with C. sinensis metacercariae were evaluated over time. Stool was analyzed by 16S rRNA amplicon analysis using high-throughput sequencing technology. There was no apparent difference in species richness and diversity between the infected and control groups. However, the composition of the microbiome was different between the infected and control groups at 20 days and 30 days post-infection, and the difference disappeared at 50 days post-infection. In particular, this microbiome alteration was associated with a change in the relative abundance of genus Lactobacillus and the probiotic Lactobacillus species that are known to have an immune-modulation role in immune-mediated diseases.

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Funding

This study was supported by a National Research Foundation of Korea (NRF) grant funded by the Korean Government (MEST; numbers NRF-2016R1A2B4016194 and 2011-0012166).

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Authors and Affiliations

  1. Department of Environmental Medical Biology, Institute of Tropical Medicine, and Arthropods of Medical Importance Resource Bank, Yonsei University College of Medicine, Seoul, 03722, Korea

    Ju Yeong Kim, Eun-Min Kim, Myung-hee Yi, Jinyoung Lee, Seogwon Lee & Tai-Soon Yong

  2. Brain Korea 21 PLUS Project for Medical Science, Yonsei University College of Medicine, Seoul, 03722, Korea

    Ju Yeong Kim & Younjee Hwang

  3. Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, 03722, Korea

    Younjee Hwang & Dongeun Yong

  4. Department of Parasitology and Tropical Medicine, and Institute of Health Sciences, Gyeongsang National University College of Medicine, Jinju, 52727, Korea

    Woon-Mok Sohn

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  1. Ju Yeong Kim
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Correspondence to Tai-Soon Yong.

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The authors declare that they have no conflict of interest.

Research involving animals

All animal protocols were approved by the Institutional Animal Care and Use Committee at Yonsei University College of Medicine (No. 2015–0339). Animal experiments were carried out in animal biosafety level-3 (ABL-3) facilities in accordance with standard management practices.

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Fig. S1

Box plots of relative abundance of Lactobacillus over time. * p < 0.05 in Wilcoxon rank-sum test. (PDF 33.7 kb)

Fig. S2

Eggs of Clonorchis sinensis in liver section of infected mouse at 50 days post-infection (hematoxylin and eosin staining, 400x). Black arrows indicate the eggs. (PDF 209 kb)

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Kim, J.Y., Kim, EM., Yi, Mh. et al. Chinese liver fluke Clonorchis sinensis infection changes the gut microbiome and increases probiotic Lactobacillus in mice. Parasitol Res 118, 693–699 (2019). https://doi.org/10.1007/s00436-018-6179-x

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