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Table 1 Comparison of mean cycle threshold (Ct) and standard deviation (SD) values between sample preparation procedures with or without bead beating

From: Improved detection of DNA Schistosoma haematobium from eggs extracted by bead beating in urine

N eggs/10 mL
(microscopy counts)
N samples Ct A_PCR vs Ct B_PCR
mean (± SD)
P value
0 5 NV vs NV NA
1–2 3 NV vs 28 (± 3.60) NA
1–2 6 33 (± 4.02) vs 30 (± 3.53) ns
5–49 8 31 (± 3.51) vs 25 (± 1.90) 0.0007
≥ 50 3 29 (± 1.76) vs 22 (± 1.15) 0.0049
  1. A_PCR, PCR resulted from directly frozen sample; B_PCR, PCR resulted from bead beating supplemented on frozen sample; NV, negative value; NA, not applicable. Unpaired parametric two-tailed Student’s t test (GraphPad Prism v7.02) was performed, and the P value ≤ 0.050 was considered significant; ns, not significant