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Phylogeny of hymenolepidid cestodes (Cestoda: Cyclophyllidea) from mammalian hosts based on partial 28S rDNA, with focus on parasites from shrews

  • Boyko Neov
  • Gergana P. Vasileva
  • Georgi Radoslavov
  • Peter Hristov
  • D. Timothy J. Littlewood
  • Boyko B. Georgiev
Original Paper
  • 53 Downloads

Abstract

The aims of the study are to enrich the partial 28S rDNA dataset for hymenolepidids by adding new sequences for species parasitic in the genera Sorex, Neomys and Crocidura (Soricidae) and to propose a new hypothesis for the relationships among mammalian hymenolepidids. New sequences were obtained for Coronacanthus integrus, C. magnihamatus, C. omissus, C. vassilevi, Ditestolepis diaphana, Lineolepis scutigera, Spasskylepis ovaluteri, Staphylocystis tiara, S. furcata, S. uncinata, Vaucherilepis trichophorus and Neoskrjabinolepis sp. The phylogenetic analysis (based on 56 taxa) confirmed the major clades identified by Haukisalmi et al. (Zool Scr 39:631–641, 2010) based on analysis of 31 species: Ditestolepis clade, Hymenolepis clade, Rodentolepis clade and Arostrilepis clade; however, the support was weak for the early divergent lineages of the tree and for the Arostrilepis clade. Novelties revealed include the molecular evidence for the monophyly of Coronacanthus, the non-monophyletic status of Staphylocystis and the polyphyly of Staphylocystoides. The analysis has confirmed the monophyly of Hymenolepis, the monophyly of hymenolepidids from glirids, the position of Pararodentolepis and Nomadolepis as sister taxa, the polyphyly of Rodentolepis, the position of Neoskrjabinolepis and Lineolepis as sister taxa, and the close relationship among the genera with the entire reduction of rostellar apparatus. Resolved monophyletic groups are supported by the structure of the rostellar apparatus. The diversification of the Ditestolepis clade is associated with soricids. The composition of the other major clades suggests multiple evolutionary events of host switching, including between different host orders. The life cycles of Coronacanthus and Vaucherilepis are recognised as secondarily aquatic as these taxa are nested in terrestrial groups.

Keywords

Phylogeny Taxonomy Hymenolepididae 28S rDNA gene 

Notes

Acknowledgements

We are grateful to the staff of the Kalimok Field Station of the Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences, for facilitating partly field studies. Sampling of host individuals was permitted by the Ministry of Environment and Waters of the Republic of Bulgaria, licences NSZP-153/11.05.2012 and NSZP-350/11.09.2014.

Funding

The present study used facilities developed at the Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences, in the frames of the projects WETLANET (EC FP7, Programme CAPACITIES, Grant 229802) and CEBDER (National Science Fund of the Republic of Bulgaria, Grant DOO2-15/17.02.2009). This work was partly funded by the National Science Foundation, PBI grants DEB 0818696 and DEB 0818823.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

The authors carefully reviewed the ethical standards of the journal and hereby certify that the procedures used with the investigated species comply fully with those standards. The methods used in the current study were approved by the Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Boyko Neov
    • 1
  • Gergana P. Vasileva
    • 1
  • Georgi Radoslavov
    • 1
  • Peter Hristov
    • 1
  • D. Timothy J. Littlewood
    • 2
  • Boyko B. Georgiev
    • 1
  1. 1.Institute of Biodiversity and Ecosystem ResearchBulgarian Academy of SciencesSofiaBulgaria
  2. 2.Department of Life SciencesThe Natural History MuseumLondonUK

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