Morphological and genetic characterisation of Sarcocystis halieti from the great cormorant (Phalacrocorax carbo)

Prakas, Petras; Butkauskas, Dalius; Švažas, Saulius; Stanevičius, Vitas

doi:10.1007/s00436-018-6083-4

Short Communication
Published: 18 September 2018

Morphological and genetic characterisation of Sarcocystis halieti from the great cormorant (Phalacrocorax carbo)

Petras Prakas ORCID: orcid.org/0000-0002-5115-2659¹,
Dalius Butkauskas¹,
Saulius Švažas¹ &
Vitas Stanevičius¹

Parasitology Research volume 117, pages 3663–3667 (2018)Cite this article

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Abstract

Having examined 19 great cormorants (Phalacrocorax carbo) hunted in Lithuania, sarcocysts were found in the muscles of two birds. Sarcocysts detected were examined using light microscopy (LM), transmission electron microscopy (TEM), and 18S rDNA, 28S rDNA, ITS1, cox1, and rpoB sequence comparison. Based on the molecular analysis, mainly of the ITS1 region, sarcocysts were identified as Sarcocystis halieti. This is the first Sarcocystis species characterised in the great cormorant. Under the LM sarcocysts were ribbon-shaped, very long and thin (the largest fragment found amounted to 6.5 × 0.1 mm) with a smooth and thin (up to 1.2 μm) cyst wall. Banana-shaped bradyzoites were 7.2 × 1.9 (6.3–8.2 × 1.4–2.4) μm. Under TEM, the cyst wall was wavy, 0.8- to 1.2-μm thick. The comparison of 12 species demonstrated cox1 and rpoB to be unsuitable for the identification of Sarcocystis spp. using birds as intermediate hosts.

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Acknowledgements

The authors are grateful to Liuda Kutkienė for her valuable comments on the draft of the paper. We are grateful for valuable comments and suggestions of reviewers, significantly contributing to the improvement of our manuscript.

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Nature Research Centre, Akademijos 2, LT-08412, Vilnius, Lithuania
Petras Prakas, Dalius Butkauskas, Saulius Švažas & Vitas Stanevičius

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Petras Prakas
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Dalius Butkauskas
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Saulius Švažas
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Vitas Stanevičius
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Correspondence to Petras Prakas.

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Prakas, P., Butkauskas, D., Švažas, S. et al. Morphological and genetic characterisation of Sarcocystis halieti from the great cormorant (Phalacrocorax carbo). Parasitol Res 117, 3663–3667 (2018). https://doi.org/10.1007/s00436-018-6083-4

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Received: 13 April 2018
Accepted: 06 September 2018
Published: 18 September 2018
Issue Date: November 2018
DOI: https://doi.org/10.1007/s00436-018-6083-4

Keywords

Sarcocystis halieti
Great cormorant
Transmission electron microscopy
rRNA
ITS1
cox1
rpoB