Skip to main content
SpringerLink
Log in

Leishmanicidal activity of α-bisabolol from Tunisian chamomile essential oil

Parasitology Research Aims and scope Submit manuscript

Cite this article

Abstract

According to the World Health Organization, leishmaniasis is considered as a major neglected tropical disease causing an enormous impact on global public health. Available treatments were complicated due to the high resistance, toxicity, and high cost. Therefore, the search for novel sources of anti-leishmania agents is an urgent need. In the present study, an in vitro evaluation of the leishmanicidal activity of the essential oil of Tunisian chamomile (Matricaria recutita L.) was carried out. Chamomile essential oil exhibits a good activity on promastigotes forms of L. amazonensis and L. infantum with a low inhibitory concentration at 50% (IC50) (10.8 ± 1.4 and 10.4 ± 0.6 μg/mL, respectively). Bio-guided fractionation was developed and led to the identification of (−)-α-bisabolol as the most active molecule with low IC50 (16.0 ± 1.2 and 9.5 ± 0.1 μg/mL for L. amazonensis and L. infantum, respectively). This isolated sesquiterpene alcohol was studied for its activity on amastigotes forms (IC50 = 5.9 ± 1.2 and 4.8 ± 1.3 μg/mL, respectively) and its cytotoxicity (selectivity indexes (SI) were 5.4 and 6.6, respectively). The obtained results showed that (−)-α-bisabolol was able to activate a programmed cell death process in the promastigote stage of the parasite. It causes phosphatidylserine externalization and membrane damage. Moreover, it decreases the mitochondrial membrane potential and total ATP levels. These results highlight the potential use of (−)-α-bisabolol against both L. amazonensis and L. infantum, and further studies should be undertaken to establish it as novel leishmanicidal therapeutic agents.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8

References

  • Almeida-Souza F, de Souza Cda S, Taniwaki NN, Silva JJ, de Oliveira RM, Abreu-Silva AL, Calabrese Kda S (2016) Morinda citrifolia Linn. fruit (Noni) juice induces an increase in NO production and death of Leishmania amazonensis amastigotes in peritoneal macrophages from BALB/c. Nitric Oxide 58:51–58

    Article  PubMed  CAS  Google Scholar 

  • Aloui Z, Messaoud C, Haoues M, Neffati N, Bassoumi Jamoussi I, Essafi-Benkhadir K, Boussaid M, Guizani I, Karoui H (2016) Asteraceae Artemisia campestris and Artemisia herba-alba essential oils trigger apoptosis and cell cycle arrest in Leishmania infantum promastigotes. Evid Based Complement Alternat Med 2016:9147096

    Article  PubMed  PubMed Central  Google Scholar 

  • Alvar J, Vélez ID, Bern C, Herrero M, Desjeux P, Cano J, Jannin J, den Boer M, WHO Leishmaniasis Control Team (2012) Leishmaniasis worldwide and global estimates of its incidence. PLoS One 7(5):e35671

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Alvar J, Yactayo S, Bern C (2006) Leishmaniasis and poverty. Trends Parasitol 22(12):552–557

    Article  PubMed  Google Scholar 

  • Andrade MA, Azevedo CD, Motta FN, Santos ML, Silva CL, Santana JM, Bastos IM (2016) Essential oils: in vitro activity against Leishmania amazonensis, cytotoxicity and chemical composition. BMC Complement Altern Med 16(1):444

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Aoun K, Bouratbine A (2014) Cutaneous leishmaniasis in North Africa: a review. Parasite 21:14

    Article  PubMed  PubMed Central  Google Scholar 

  • Bashaye S, Nombela N, Argaw D, Mulugeta A, Herrero M, Nieto J, Chicharro C, Canavate C, Aparicio P, Velez ID, Alvar J, Bern C (2009) Risk factors for visceral leishmaniasis in a new epidemic site in Amhara Region, Ethiopia. Am J Trop Med Hyg 81(1):34–39

    Article  PubMed  Google Scholar 

  • Bhatia SP, McGinty D, Letizia CS, Api AM (2008) Fragrance material review on α-bisabolol. Food Chem Toxicol 46(11):S72–S76

    Article  PubMed  CAS  Google Scholar 

  • Boelaert M, Criel B, Leeuwenburg J, Van Damme W, Le Ray D, Van der Stuyft P (2000) Visceral leishmaniasis control: a public health perspective. Trans R Soc Trop Med Hyg 94(5):465–471

    Article  PubMed  CAS  Google Scholar 

  • Boelaert M, Meheus F, Sanchez A, Singh SP, Vanlerberghe V, Picado A, Meessen B, Sundar S (2009) The poorest of the poor: a poverty appraisal of households affected by visceral leishmaniasis in Bihar, India. Tropical Med Int Health 14(6):639–644

    Article  CAS  Google Scholar 

  • Bousslimi N, Ben Abda I, Ben Mously R, Siala E, Harrat Z, Zallagua N, Bouratbine A, Aoun K (2014) Contribution of Leishmania identification using polymerase chain reaction—restriction fragment length polymerase for epidemiological studies of cutaneous leishmaniasis in Tunisia. Pathol Biol 62(1):30–33

    Article  PubMed  CAS  Google Scholar 

  • Bradley P (1992) British herbal compendium. Volume 1. A handbook of scientific information on widely used plant drugs. Companion to Volume 1 of the British Herbal Pharmacopoeia. British Herbal Medicine Association, Bournemouth

    Google Scholar 

  • Cabrera-Serra MG, Lorenzo-Morales J, Romero M, Valladares B, Pinero JE (2007) In vitro activity of perifosine: a novel alkylphospholipid against the promastigote stage of Leishmania species. Parasitol Res 100(5):1155–1157

    Article  PubMed  Google Scholar 

  • Cavalieri E, Mariotto S, Fabrizi C, de Prati AC, Gottardo R, Leone S, Berra LV, Lauro GM, Ciampa AR, Suzuki H (2004) Alpha-bisabolol, a nontoxic natural compound, strongly induces apoptosis in glioma cells. Biochem Biophys Res Commun 315(3):589–594

    Article  PubMed  CAS  Google Scholar 

  • Cerceau CI, Barbosa LCA, Alvarenga ES, Ferreira AG, Thomasi SS (2016) A validated 1H NMR method for quantitative analysis of α-bisabolol in essential oils of Eremanthus erythropappus. Talanta 161:71–79

    Article  PubMed  CAS  Google Scholar 

  • Chen W, Hou J, Yin Y, Jang J, Zheng Z, Fan H, Zou G (2010) Alpha-bisabolol induces dose- and time-dependent apoptosis in HepG2 cells via a Fas- and mitochondrial-related pathway involves p53 and NFkappaB. Biochem Pharmacol 80(2):247–254

    Article  PubMed  CAS  Google Scholar 

  • Corpas-López V, Merino-Espinosa G, Diaz-Saez V, Morillas-Marquez F, Navarro-Moll MC, Martin-Sanchez J (2016a) The sesquiterpene (−)-alpha-bisabolol is active against the causative agents of Old World cutaneous leishmaniasis through the induction of mitochondrial-dependent apoptosis. Apoptosis 21(10):1071–1081

    Article  PubMed  CAS  Google Scholar 

  • Corpas-López V, Merino-Espinosa G, López-Viota M, Gijon-Robles P, Morillas-Mancilla MJ, López-Viota J, Diaz-Saez V, Morillas-Marquez F, Navarro Moll MC, Martin-Sanchez J (2016b) Topical treatment of Leishmania tropica infection using (−) alpha-bisabolol ointment in a hamster model: effectiveness and safety assessment. J Nat Prod 79(9):2403–2407

    Article  PubMed  CAS  Google Scholar 

  • Corpas-López V, Morillas-Marquez F, Navarro-Moll MC, Merino-Espinosa G, Diaz-Saez V, Martin-Sanchez J (2015) (−)Alpha-bisabolol, a promising oral compound for the treatment of visceral leishmaniasis. J Nat Prod 78(6):1202–1207

    Article  PubMed  CAS  Google Scholar 

  • Croft SL, Olliaro P (2011) Leishmaniasis chemotherapy-challenges and opportunities. Clin Microbiol Infect 17(10):1478–1483

    Article  PubMed  CAS  Google Scholar 

  • Das M, Mukherjee SB, Shaha C (2001) Hydrogen peroxide induces apoptosis-like death in Leishmania donovani promastigotes. J Cell Sci 114(13):2461–2469

    PubMed  CAS  Google Scholar 

  • de Medeiros M, da Silva AC, Cito AM, Borges AR, de Lima SG, Lopes JA, Figueiredo RC (2011) In vitro antileishmanial activity and cytotoxicity of essential oil from Lippia sidoides Cham. Parasitol Int 60(3):237–241

    Article  PubMed  CAS  Google Scholar 

  • Desjeux P (1996) Leishmaniasis. Public health aspects and control. Clin Dermatol 14(5):417–423

    Article  PubMed  CAS  Google Scholar 

  • Desjeux P (2004) Leishmaniasis: current situation and new perspectives. Comp Immunol Microbiol Infect Dis 27(5):305–318

    Article  PubMed  CAS  Google Scholar 

  • Fidalgo LM, Gille L (2011) Mitochondria and trypanosomatids: targets and drugs. Pharm Res 28(11):2758–2770

    Article  PubMed  CAS  Google Scholar 

  • Ganzera M, Schneider P, Stuppner H (2006) Inhibitory effects of the essential oil of chamomile (Matricaria recutita L.) and its major constituents on human cytochrome P450 enzymes. Life Sci 78(8):856–861

    Article  PubMed  CAS  Google Scholar 

  • Gawde A, Cantrell CL, Zheljazkov VD, Astatkie T, Schlegel V (2014) Steam distillation extraction kinetics regression models to predict essential oil yield, composition, and bioactivity of chamomile oil. Ind Crop Prod 58:61–67

    Article  CAS  Google Scholar 

  • Gottlieb RA (2001) Mitochondria and apoptosis. Biol Signals Recept 10(3–4):147–161

    Article  PubMed  CAS  Google Scholar 

  • Handler MZ, Patel PA, Kapila R, Al-Qubati Y, Schwartz RA (2015) Cutaneous and mucocutaneous leishmaniasis: clinical perspectives. J Am Acad Dermatol 73(6):897–908

    Article  PubMed  Google Scholar 

  • Herwaldt BL (1999) Leishmaniasis. Lancet 354(9185):1191–1199

    Article  PubMed  CAS  Google Scholar 

  • Inacio JD, Gervazoni L, Canto-Cavalheiro MM, Almeida-Amaral EE (2014) The effect of (−)-epigallocatechin 3-O-gallate in vitro and in vivo in Leishmania braziliensis: involvement of reactive oxygen species as a mechanism of action. PLoS Negl Trop Dis 8(8):e3093

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Jabri M-A, Hajaji S, Marzouki L, El-Benna J, Sakly M, Sebai H (2016) Human neutrophils ROS inhibition and protective effects of Myrtus communis leaves essential oils against intestinal ischemia/reperfusion injury. RSC Adv 6:16645–16655

    Article  CAS  Google Scholar 

  • Jain SK, Sahu R, Walker LA, Tekwani BL (2012) A parasite rescue and transformation assay for antileishmanial screening against intracellular Leishmania donovani amastigotes in THP1 human acute monocytic leukemia cell line. J Vis Exp 70:4054

    Google Scholar 

  • Jamalian A, Shams-Ghahfarokhi M, Jaimand K, Pashootan N, Amani A, Razzaghi-Abyaneh M (2012) Chemical composition and antifungal activity of Matricaria recutita flower essential oil against medically important dermatophytes and soil-borne pathogens. J Mycol Med 22(4):308–315

    Article  PubMed  CAS  Google Scholar 

  • Kathuria M, Bhattacharjee A, Sashidhara KV, Singh SP, Mitra K (2014) Induction of mitochondrial dysfunction and oxidative stress in Leishmania donovani by orally active clerodane diterpene. Antimicrob Agents Chemother 58(10):5916–5928

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Kbaier-Hachemi H, Barhoumi M, Chakroun AS, Ben Fadhel M, Guizani I (2008) Differentiation among cutaneous Leishmania species upon amplification of a sequence of dipeptidyl peptidase III encoding gene. Arch Inst Pasteur Tunis 85(1–4):45–53

    PubMed  CAS  Google Scholar 

  • Kulkarni MM, McMaster WR, Kamysz W, McGwire BS (2009) Antimicrobial peptide-induced apoptotic death of leishmania results from calcium-dependent, caspase-independent mitochondrial toxicity. J Biol Chem 284(23):15496–15504

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Likhitwitayawuid K, Angerhofer CK, Chai H, Pezzuto JM, Cordell GA, Ruangrungsi N (1993) Cytotoxic and antimalarial alkaloids from the tubers of Stephania pierrei. J Nat Prod 56(9):1468–1478

    Article  PubMed  CAS  Google Scholar 

  • López-Arencibia A, García-Velázquez D, Martín-Navarro CM, Sifaoui I, Reyes-Batlle M, Lorenzo-Morales J, Gutiérrez-Ravelo Á, Piñero JE (2015) In vitro activities of hexaazatrinaphthylenes against Leishmania spp. Antimicrob Agents Chemother 59(5):2867–2874

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • López-Arencibia A, Martín-Navarro C, Sifaoui I, Reyes-Batlle M, Wagner C, Lorenzo-Morales J, Maciver SK, Piñero JE (2017) Perifosine mechanisms of action in Leishmania species. Antimicrob Agents Chemother 61(4):e02127–e02143

    Article  PubMed  PubMed Central  Google Scholar 

  • Lorenzo-Morales J, Martin-Navarro CM, López-Arencibia A, Santana-Morales MA, Afonso-Lehmann RN, Maciver SK, Valladares B, Martinez-Carretero E (2010) Therapeutic potential of a combination of two gene-specific small interfering RNAs against clinical strains of Acanthamoeba. Antimicrob Agents Chemother 54(12):5151–5155

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Maltezou HC (2010) Drug resistance in visceral leishmaniasis. J Biomed Biotechnol 2010:617521

    Article  PubMed  CAS  Google Scholar 

  • McKay DL, Blumberg JB (2006) A review of the bioactivity and potential health benefits of chamomile tea (Matricaria recutita L.). Phytother Res 20(7):519–530

    Article  PubMed  CAS  Google Scholar 

  • Mehta A, Shaha C (2006) Mechanism of metalloid-induced death in Leishmania spp.: role of iron, reactive oxygen species, Ca2+, and glutathione. Free Radic Biol Med 40(10):1857–1868

    Article  PubMed  CAS  Google Scholar 

  • Morales-Yuste M, Morillas-Marquez F, Martin-Sanchez J, Valero-López A, Navarro-Moll MC (2010) Activity of (−)alpha-bisabolol against Leishmania infantum promastigotes. Phytomedicine 17(3–4):279–281

    Article  PubMed  CAS  Google Scholar 

  • Nissanka APK, Karunaratne V, Bandara BMR, Kumar V, Nakanishi T, Nishi M, Inada A, Tillekeratne LMV, Wijesundara DSA, Gunatilaka AAL (2001) Antimicrobial alkaloids from Zanthoxylum tetraspermum and caudatum. Phytochemistry 56:857–861

    Article  PubMed  CAS  Google Scholar 

  • Nuydens R, Novalbos J, Dispersyn G, Weber C, Borgers M, Geerts H (1999) A rapid method for the evaluation of compounds with mitochondria-protective properties. J Neurosci Methods 92(1–2):153–159

    Article  PubMed  CAS  Google Scholar 

  • O'Brien J, Wilson I, Orton T, Pognan F (2000) Investigation of the Alamar Blue (resazurin) fluorescent dye for the assessment of mammalian cell cytotoxicity. Eur J Biochem 267(17):5421–5426

    Article  PubMed  CAS  Google Scholar 

  • Oryan A, Akbari M (2016) Worldwide risk factors in leishmaniasis. Asian Pac J Trop Med 9(10):925–932

    Article  PubMed  CAS  Google Scholar 

  • Peters BS, Fish D, Golden R, Evans DA, Bryceson AD, Pinching AJ (1990) Visceral leishmaniasis in HIV infection and AIDS: clinical features and response to therapy. Q J Med 77(283):1101–1111

    Article  PubMed  CAS  Google Scholar 

  • Piochon M, Legault J, Gauthier C, Pichette A (2009) Synthesis and cytotoxicity evaluation of natural α-bisabolol β-d-fucopyranoside and analogues. Phytochemistry 70(2):228–236

    Article  PubMed  CAS  Google Scholar 

  • Rasmussen RE, Erstad SM, Ramos-Martinez EM, Fimognari L, De Porcellinis AJ, Sakuragi Y (2016) An easy and efficient permeabilization protocol for in vivo enzyme activity assays in cyanobacteria. Microb Cell Factories 15(1):186

    Article  CAS  Google Scholar 

  • Reithinger R, Dujardin JC, Louzir H, Pirmez C, Alexander B, Brooker S (2007) Cutaneous leishmaniasis. Lancet Infect Dis 7(9):581–596

    Article  PubMed  Google Scholar 

  • Rigo A, Vinante F (2016) The antineoplastic agent alpha-bisabolol promotes cell death by inducing pores in mitochondria and lysosomes. Apoptosis 21(8):917–927

    Article  PubMed  CAS  Google Scholar 

  • Roby MHH, Sarhan MA, Selim KAH, Khalel KI (2013) Antioxidant and antimicrobial activities of essential oil and extracts of fennel (Foeniculum vulgare L.) and chamomile (Matricaria chamomilla L.). Ind Crop Prod 44:437–445

    Article  CAS  Google Scholar 

  • Rosa MSS, Mendonc-a-Filho RR, Bizzo HR, Rodrigues IA, Soares RMA, Souto-Padron T, Alviano CS, Lopes ACHS (2003) Antileishmanial activity of a linalool-rich essential oil from Croton cajucara. Antimicrob Agents Chemother 47:1895–1901

    Article  PubMed Central  CAS  Google Scholar 

  • Rottini MM, Amaral AC, Ferreira JL, Silva JR, Taniwaki NN, Souza Cda S, d’Escoffier LN, Almeida-Souza F, Hardoim Dde J, Goncalves da Costa SC, Calabrese Kda S (2015) In vitro evaluation of (−)alpha-bisabolol as a promising agent against Leishmania amazonensis. Exp Parasitol 148:66–72

    Article  PubMed  CAS  Google Scholar 

  • Roy A, Ganguly A, BoseDasgupta S, Das BB, Pal C, Jaisankar P, Majumder HK (2008) Mitochondria-dependent reactive oxygen species-mediated programmed cell death induced by 3,3′-diindolylmethane through inhibition of F0F1-ATP synthase in unicellular protozoan parasite Leishmania donovani. Mol Pharmacol 74(5):1292–1307

    Article  PubMed  CAS  Google Scholar 

  • Salomao K, De Santana NA, Molina MT, De Castro SL, Menna-Barreto RF (2013) Trypanosoma cruzi mitochondrial swelling and membrane potential collapse as primary evidence of the mode of action of naphthoquinone analogues. BMC Microbiol 13:196

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Shirian S, Oryan A, Hatam GR, Daneshbod Y (2013) Three Leishmania/L. species—L. infantum, L. major, L. tropica—as causative agents of mucosal leishmaniasis in Iran. Patholog Glob Health 107(5):267–272

    Article  Google Scholar 

  • Sifaoui I, López-Arencibia A, Martin-Navarro CM, Ticona JC, Reyes-Batlle M, Mejri M, Jimenez AI, López-Bazzocchi I, Valladares B, Lorenzo-Morales J, Abderabba M, Pinero JE (2014) In vitro effects of triterpenic acids from olive leaf extracts on the mitochondrial membrane potential of promastigote stage of Leishmania spp. Phytomedicine 21(12):1689–1694

    Article  PubMed  CAS  Google Scholar 

  • Sikkema J, de Bont JA, Poolman B (1995) Mechanisms of membrane toxicity of hydrocarbons. Microbiol Rev 59(2):201–222

    PubMed  PubMed Central  CAS  Google Scholar 

  • Singh B, Sundar S (2012) Leishmaniasis: vaccine candidates and perspectives. Vaccine 30(26):3834–3842

    Article  PubMed  CAS  Google Scholar 

  • Tempone AG, Martins de Oliveira C, Berlinck RG (2011) Current approaches to discover marine antileishmanial natural products. Planta Med 77(6):572–585

    Article  PubMed  CAS  Google Scholar 

  • Tiuman TS, Ueda-Nakamura T, Alonso A, Nakamura CV (2014) Cell death in amastigote forms of Leishmania amazonensis induced by parthenolide. BMC Microbiol 14:152

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Tolouee M, Alinezhad S, Saberi R, Eslamifar A, Zad SJ, Jaimand K, Taeb J, Rezaee MB, Kawachi M, Shams-Ghahfarokhi M, Razzaghi-Abyaneh M (2010) Effect of Matricaria chamomilla L. flower essential oil on the growth and ultrastructure of Aspergillus niger van Tieghem. Int J Food Microbiol 139(3):127–133

    Article  PubMed  CAS  Google Scholar 

  • Verma NK, Singh G, Dey CS (2007) Miltefosine induces apoptosis in arsenite-resistant Leishmania donovani promastigotes through mitochondrial dysfunction. Exp Parasitol 116(1):1–13

    Article  PubMed  CAS  Google Scholar 

  • WHO (2010) Control of the leishmaniases. World Health Organ Tech Rep Ser 949:1–186

    Google Scholar 

  • WHO (2013) http://www.who.int/gho/neglected_diseases/leishmaniasis/en/

  • Yamamoto ES, Campos BL, Jesus JA, Laurenti MD, Ribeiro SP, Kallas EG, Rafael-Fernandes M, Santos-Gomes G, Silva MS, Sessa DP, Lago JH, Levy D, Passero LF (2015) The effect of ursolic acid on Leishmania (Leishmania) amazonensis is related to programed cell death and presents therapeutic potential in experimental cutaneous leishmaniasis. PLoS One 10(12):e0144946

    Article  PubMed  PubMed Central  CAS  Google Scholar 

Download references

Funding

This work was supported by the grants RICET (project no. RD12/0018/0012 of the program of Redes Temáticas de Investigación Cooperativa, FIS), Spanish Ministry of Health, Madrid, Spain, the Project PI13/00490 “Protozoosis Emergentes por Amebas de Vida Libre: Aislamiento, Caracterización, Nuevas Aproximaciones Terapéuticas y Traslación Clínica de los Resultados” from the Instituto de Salud Carlos III, and Project ref. AGUA3 “Amebas de Vida Libre como Marcadores de Calidad del Agua” from CajaCanarias Fundación. SH was supported by the “Laboratoire d’Epidémiologie d’Infections Enzootiques des Herbivores en Tunisie” (Ministère de l’enseignement supérieur, Tunisia) and by the “Ayudas para estancias de estudiantes de posgrado e investigadores americanos y africanos—2016” of the University of La Laguna. JLM was supported by the Ramón y Cajal Subprogramme from the Spanish Ministry of Economy and Competitivity RYC-2011-08863. ILB and IAJ were supported by the SAF2015-65113-C2-1-R MINECO, Spain Project and by FEDER funds from the EU. IS and ALA were supported by the Agustin de Bethancourt Programme.

Author information

Authors and Affiliations

  1. Laboratoire de Parasitologie, École Nationale de Médecine Vétérinaire de Sidi Thabet, Université de la Manouba, 2020, Manouba, Tunisie

    Soumaya Hajaji & Hafidh Akkari

  2. Faculté des sciences de Bizerte, Université de Carthage, 7021, Zarzouna, Tunisie

    Soumaya Hajaji

  3. Laboratoire Matériaux-Molécules et Applications, IPEST, University of Carthage, La Marsa, Tunisia

    Ines Sifaoui

  4. University Institute of Tropical Diseases and Public Health of the Canary Islands, University of La Laguna, Avda. Astrofísico Fco. Sánchez, La Laguna, Tenerife, Canary Islands, Spain

    Ines Sifaoui, Atteneri López-Arencibia, María Reyes-Batlle, Basilio Valladares, Jacob Lorenzo-Morales & José E. Piñero

  5. Instituto Universitario de Bio-Orgánica Antonio González, Departamento Química Orgánica, Universidad de La Laguna, Tenerife, Canary Islands, Spain

    Ignacio A. Jiménez & Isabel L. Bazzocchi

Authors
  1. Soumaya Hajaji
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ines Sifaoui
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Atteneri López-Arencibia
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. María Reyes-Batlle
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Ignacio A. Jiménez
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Isabel L. Bazzocchi
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Basilio Valladares
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Hafidh Akkari
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Jacob Lorenzo-Morales
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. José E. Piñero
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Soumaya Hajaji.

Additional information

Section Editor: Sarah Hendrickx

Rights and permissions

Reprints and Permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Hajaji, S., Sifaoui, I., López-Arencibia, A. et al. Leishmanicidal activity of α-bisabolol from Tunisian chamomile essential oil. Parasitol Res 117, 2855–2867 (2018). https://doi.org/10.1007/s00436-018-5975-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00436-018-5975-7

Keywords

search

Navigation